BRENDA - Enzyme Database
show all sequences of 3.6.1.B16

Molecular characterization of organelle-type Nudix hydrolases in Arabidopsis

Ogawa, T.; Yoshimura, K.; Miyake, H.; Ishikawa, K.; Ito, D.; Tanabe, N.; Shigeoka, S.; Plant Physiol. 148, 1412-1424 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
activity increases in presence of 1 mM dithiothreitol
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0096
-
P1,P5-di(adenosine-5')pentaphosphate
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
0.0218
-
P1,P4-bis(5'-adenosyl) tetraphosphate
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9CAF2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
730635
Arabidopsis thaliana
?
-
-
-
-
P1,P5-di(adenosine-5')pentaphosphate + H2O
-
730635
Arabidopsis thaliana
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.8
-
P1,P5-di(adenosine-5')pentaphosphate
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
16.9
-
P1,P4-bis(5'-adenosyl) tetraphosphate
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
activity increases in presence of 1 mM dithiothreitol
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0096
-
P1,P5-di(adenosine-5')pentaphosphate
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
0.0218
-
P1,P4-bis(5'-adenosyl) tetraphosphate
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
730635
Arabidopsis thaliana
?
-
-
-
-
P1,P5-di(adenosine-5')pentaphosphate + H2O
-
730635
Arabidopsis thaliana
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.8
-
P1,P5-di(adenosine-5')pentaphosphate
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
16.9
-
P1,P4-bis(5'-adenosyl) tetraphosphate
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
physiological function
T-DNA insertion mutants do not exhibit any phenotypical differences under normal growth conditions. The gene NUDX26 cannot complement the mutator phenotype of the Escherichia coli mutT- strain
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
T-DNA insertion mutants do not exhibit any phenotypical differences under normal growth conditions. The gene NUDX26 cannot complement the mutator phenotype of the Escherichia coli mutT- strain
Arabidopsis thaliana
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
770
-
AppppA
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
1200
-
ApppppA
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
770
-
AppppA
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
1200
-
ApppppA
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
Arabidopsis thaliana
Other publictions for EC 3.6.1.B16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729429
Ito
Enzymatic and molecular charac ...
Arabidopsis thaliana
Biosci. Biotechnol. Biochem.
76
2236-2241
2012
-
-
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-
-
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1
1
2
-
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1
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1
-
1
-
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1
1
2
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1
1
2
-
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-
-
-
-
-
-
1
-
1
-
-
1
1
2
-
-
1
-
-
1
1
1
730635
Ogawa
Molecular characterization of ...
Arabidopsis thaliana
Plant Physiol.
148
1412-1424
2008
1
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
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2
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2
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1
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2
1
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2
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2
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1
1
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2
2