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Literature summary for 3.6.1.9 extracted from

  • Kaneko, K.; Inomata, T.; Masui, T.; Koshu, T.; Umezawa, Y.; Itoh, K.; Pozueta-Romero, J.; Mitsui, T.
    Nucleotide pyrophosphatase/phosphodiesterase 1 exerts a negative effect on starch accumulation and growth in rice seedlings under high temperature and CO2 concentration conditions (2014), Plant Cell Physiol., 55, 320-332.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene npp1, ectopic expression in transgenic rice suspension cells Oryza sativa
gene npp2, screening of a rice shoot cDNA library and cDNAs sequencing encoding NPP isozyme genes, ectopic expression of npp2 in transgenic rice suspension cells Oryza sativa
gene npp6, screening of a rice shoot cDNA library and cDNAs sequencing encoding NPP isozyme genes, ectopic expression of npp6 in transgenic rice suspension cells Oryza sativa

Protein Variants

Protein Variants Comment Organism
additional information generation of an npp1 knockout mutant, that shows shoots and roots larger than those of wild-type seedlings. The starch content in the npp1 mutant shoots is higher than that of wild-type shoots. Growth and starch accumulation are also enhanced under an atmospheric CO2 concentration (40 Pa) when plants were cultured under a 33°C/28°C regime Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
ADP pH 4.5, 37°C Oryza sativa
0.5
-
diphosphate pH 4.5, 37°C Oryza sativa
0.5
-
UDP pH 4.5, 37°C Oryza sativa
0.5
-
adenosine 5'-phosphosulfate pH 4.5, 37°C Oryza sativa
0.7
-
ADP-glucose pH 4.5, 37°C Oryza sativa
0.8
-
GDP-mannose pH 6.0, 37°C Oryza sativa
0.8
-
ADP-glucose pH 6.0, 37°C Oryza sativa
0.8
-
adenosine 5'-phosphosulfate pH 4.5, 37°C Oryza sativa
0.9
-
CDP-glucose pH 6.0, 37°C Oryza sativa
0.9
-
GDP pH 4.5, 37°C Oryza sativa
1.1
-
ATP pH 4.5, 37°C Oryza sativa
1.1
-
TDP pH 4.5, 37°C Oryza sativa
1.2
-
UDP pH 4.5, 37°C Oryza sativa
1.4
-
ADP-ribose pH 6.0, 37°C Oryza sativa
1.5
-
UDP-glucose pH 6.0, 37°C Oryza sativa
1.6
-
UDP-glucose pH 4.5, 37°C Oryza sativa
1.8
-
TDP-glucose pH 4.5, 37°C Oryza sativa
1.9
-
CDP pH 4.5, 37°C Oryza sativa
2.1
-
ATP pH 6.0, 37°C Oryza sativa
2.1
-
bis(4-nitrophenyl)phosphate pH 6.0, 37°C Oryza sativa
2.2
-
diphosphate pH 4.5, 37°C Oryza sativa
2.2
-
IDP pH 4.5, 37°C Oryza sativa
2.6
-
TDP pH 4.5, 37°C Oryza sativa
2.6
-
bis(4-nitrophenyl)phosphate pH 4.5, 37°C Oryza sativa
3
-
adenosine 5'-phosphosulfate pH 6.0, 37°C Oryza sativa
3
-
bis(4-nitrophenyl)phosphate pH 4.5, 37°C Oryza sativa
4.3
-
ADP pH 6.0, 37°C Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme shows no requirement for a metal ion Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, recombinant isozyme NPP1, SDS-PAGE Oryza sativa
71000
-
x * 71000, recombinant isozyme NPP2, SDS-PAGE Oryza sativa
74000
-
x * 74000, recombinant isozyme NPP6, SDS-PAGE Oryza sativa
224000
-
recombinant isozyme NPP6, gel filtration Oryza sativa
251000
-
recombinant isozyme NPP1, gel filtration Oryza sativa
374000
-
recombinant isozyme NPP2, gel filtration Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
gene npp6
-
Oryza sativa I4DHV7 NPP2; gene npp2
-
Oryza sativa Q6ZI95 NPP1; gene npp1
-

Source Tissue

Source Tissue Comment Organism Textmining
callus
-
Oryza sativa
-
leaf mature Oryza sativa
-
additional information isozyme NPP1 is widely expressed in shoots, young roots, scutella, mature leaves and roots, but not in calli Oryza sativa
-
additional information isozyme NPP6 is expressed in the whole plant and also in calli Oryza sativa
-
root young Oryza sativa
-
root isozyme NPP2 is predominantly expressed in roots Oryza sativa
-
scutellum
-
Oryza sativa
-
seedling
-
Oryza sativa
-
shoot
-
Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 5'-phosphosulfate + H2O 31% of the activity with ADP Oryza sativa ?
-
?
adenosine 5'-phosphosulfate + H2O 44% of the activity with ADP-glucose Oryza sativa ?
-
?
adenosine 5'-phosphosulfate + H2O 81% of the activity with UDP Oryza sativa ?
-
?
ADP + H2O best substrate Oryza sativa ?
-
?
ADP + H2O 40% of the activity with ADP-glucose Oryza sativa ?
-
?
ADP + H2O 87% of the activity with UDP Oryza sativa ?
-
?
ADP-glucose + H2O best substrate Oryza sativa AMP + alpha-D-glucose 1-phosphate
-
?
ADP-glucose + H2O 65% of the activity with ADP Oryza sativa AMP + alpha-D-glucose 1-phosphate
-
?
ADP-ribose + H2O 99% of the activity with ADP-glucose Oryza sativa AMP + alpha-D-ribose 1-phosphate
-
?
ATP + H2O
-
Oryza sativa ?
-
?
ATP + H2O 81% of the activity with ADP-glucose Oryza sativa ?
-
?
bis(4-nitrophenyl)phosphate + H2O
-
Oryza sativa ?
-
?
bis(4-nitrophenyl)phosphate + H2O 32% of the activity with UDP Oryza sativa ?
-
?
bis(4-nitrophenyl)phosphate + H2O 35% of the activity with UDP Oryza sativa ?
-
?
CDP + H2O 56% of the activity with UDP Oryza sativa ?
-
?
CDP-glucose + H2O 77% of the activity with ADP-glucose Oryza sativa CMP + alpha-D-glucose 1-phosphate
-
?
diphosphate + H2O
-
Oryza sativa ?
-
?
diphosphate + H2O 59% of the activity with ADP Oryza sativa ?
-
?
GDP + H2O 38% of the activity with UDP Oryza sativa ?
-
?
GDP-mannose + H2O 76% of the activity with ADP-glucose Oryza sativa GMP + alpha-D-mannose 1-phosphate
-
?
IDP + H2O 48% of the activity with UDP Oryza sativa ?
-
?
additional information isozyme NPP1 exhibits hydrolytic activities toward ATP, UDP-glucose and the starch precursor molecule, ADP-glucose, no activity with hexose phosphates and mononucleotides Oryza sativa ?
-
?
additional information isozyme NPP2 does not recognize nucleotide sugars as substrates, but hydrolyzed UDP, ADP and adenosine 5'-phosphosulfate, no activity with hexose phosphates and mononucleotides Oryza sativa ?
-
?
additional information isozyme NPP6 exhibits hydrolytic activities toward ATP, UDP-glucose and the starch precursor molecule, ADP-glucose, no activity with hexose phosphates and mononucleotides Oryza sativa ?
-
?
TDP + H2O 27% of the activity with ADP Oryza sativa ?
-
?
TDP + H2O 40% of the activity with UDP Oryza sativa ?
-
?
TDP-glucose + H2O 15% of the activity with ADP Oryza sativa tMP + alpha-D-glucose 1-phosphate
-
?
TDP-glucose + H2O 82% of the activity with ADP-glucose Oryza sativa tMP + alpha-D-glucose 1-phosphate
-
?
UDP + H2O best substrate Oryza sativa ?
-
?
UDP + H2O 68% of the activity with ADP Oryza sativa ?
-
?
UDP-glucose + H2O 25% of the activity with ADP Oryza sativa UMP + alpha-D-glucose 1-phosphate
-
?
UDP-glucose + H2O 77% of the activity with ADP-glucose Oryza sativa UMP + alpha-D-glucose 1-phosphate
-
?

Subunits

Subunits Comment Organism
oligomer x * 70000, recombinant isozyme NPP1, SDS-PAGE Oryza sativa
oligomer x * 71000, recombinant isozyme NPP2, SDS-PAGE Oryza sativa
oligomer x * 74000, recombinant isozyme NPP6, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
NPP1
-
Oryza sativa
NPP2
-
Oryza sativa
NPP6
-
Oryza sativa
nucleotide pyrophosphatase/phosphodiesterase
-
Oryza sativa
nucleotide pyrophosphatase/phosphodiesterase 1
-
Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
isozyme NPP6 Oryza sativa
60
-
isozyme NPP1 Oryza sativa
60
-
isozyme NPP2 Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 GDP pH 4.5, 37°C Oryza sativa
34
-
adenosine 5'-phosphosulfate pH 4.5, 37°C Oryza sativa
40
-
diphosphate pH 4.5, 37°C Oryza sativa
71
-
bis(4-nitrophenyl)phosphate pH 4.5, 37°C Oryza sativa
76
-
adenosine 5'-phosphosulfate pH 4.5, 37°C Oryza sativa
79
-
diphosphate pH 4.5, 37°C Oryza sativa
80
-
ATP pH 4.5, 37°C Oryza sativa
81
-
TDP-glucose pH 4.5, 37°C Oryza sativa
83
-
ADP pH 4.5, 37°C Oryza sativa
87
-
TDP pH 4.5, 37°C Oryza sativa
88
-
IDP pH 4.5, 37°C Oryza sativa
89
-
CDP pH 4.5, 37°C Oryza sativa
101
-
UDP pH 4.5, 37°C Oryza sativa
104
-
UDP pH 4.5, 37°C Oryza sativa
115
-
UDP-glucose pH 4.5, 37°C Oryza sativa
124
-
TDP-glucose pH 6.0, 37°C Oryza sativa
125
-
CDP-glucose pH 6.0, 37°C Oryza sativa
130
-
ADP-glucose pH 4.5, 37°C Oryza sativa
151
-
GDP-mannose pH 6.0, 37°C Oryza sativa
214
-
ADP-glucose pH 6.0, 37°C Oryza sativa
223
-
bis(4-nitrophenyl)phosphate pH 6.0, 37°C Oryza sativa
282
-
UDP-glucose pH 6.0, 37°C Oryza sativa
308
-
bis(4-nitrophenyl)phosphate pH 4.5, 37°C Oryza sativa
329
-
adenosine 5'-phosphosulfate pH 6.0, 37°C Oryza sativa
370
-
ADP-ribose pH 6.0, 37°C Oryza sativa
415
-
ATP pH 6.0, 37°C Oryza sativa
449
-
ADP pH 6.0, 37°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5 isozyme NPP6 Oryza sativa
4.5
-
isozyme NPP2 Oryza sativa
6
-
isozyme NPP1 Oryza sativa

General Information

General Information Comment Organism
malfunction an npp1 knockout mutant shows shoots and roots larger than those of wild-type seedlings. The starch content in the npp1 mutant shoots is higher than that of wild-type shoots. Growth and starch accumulation are also enhanced under an atmospheric CO2 concentration (40 Pa) when plants were cultured under a 33°C/28°C regime, phenotype, overview. Downregulation of isozyme NPP1 expression is not accompanied by enhancement of AGPase, UGPase and sucrose synthase activities Oryza sativa
physiological function the enzyme exerts a negative effect on starch accumulation and growth in rice seedlings under high temperature and CO2 concentration conditions. Isozyme NPP1 is a major determinant of ADP-glucose hydrolytic activity in rice shoots Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
18
-
diphosphate pH 4.5, 37°C Oryza sativa
27
-
bis(4-nitrophenyl)phosphate pH 4.5, 37°C Oryza sativa
32
-
GDP pH 4.5, 37°C Oryza sativa
34
-
TDP pH 4.5, 37°C Oryza sativa
40
-
IDP pH 4.5, 37°C Oryza sativa
45
-
TDP-glucose pH 4.5, 37°C Oryza sativa
47
-
CDP pH 4.5, 37°C Oryza sativa
68
-
adenosine 5'-phosphosulfate pH 4.5, 37°C Oryza sativa
72
-
UDP-glucose pH 4.5, 37°C Oryza sativa
73
-
ATP pH 4.5, 37°C Oryza sativa
81
-
TDP pH 4.5, 37°C Oryza sativa
84
-
UDP pH 4.5, 37°C Oryza sativa
93
-
adenosine 5'-phosphosulfate pH 4.5, 37°C Oryza sativa
103
-
bis(4-nitrophenyl)phosphate pH 4.5, 37°C Oryza sativa
104.5
-
ADP pH 6.0, 37°C Oryza sativa
109.7
-
adenosine 5'-phosphosulfate pH 6.0, 37°C Oryza sativa
139
-
CDP-glucose pH 6.0, 37°C Oryza sativa
171.5
-
bis(4-nitrophenyl)phosphate pH 6.0, 37°C Oryza sativa
175
-
diphosphate pH 4.5, 37°C Oryza sativa
186
-
ADP-glucose pH 4.5, 37°C Oryza sativa
188
-
UDP-glucose pH 6.0, 37°C Oryza sativa
189
-
GDP-mannose pH 6.0, 37°C Oryza sativa
198
-
ATP pH 6.0, 37°C Oryza sativa
203
-
UDP pH 4.5, 37°C Oryza sativa
206
-
TDP-glucose pH 6.0, 37°C Oryza sativa
264.3
-
ADP-ribose pH 6.0, 37°C Oryza sativa
267.5
-
ADP-glucose pH 6.0, 37°C Oryza sativa
297
-
ADP pH 4.5, 37°C Oryza sativa