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Literature summary for 3.6.1.9 extracted from

  • Iqbal, J.; Levesque, S.A.; Sevigny, J.; Mueller, C.E.
    A highly sensitive CE-UV method with dynamic coating of silica-fused capillaries for monitoring of nucleotide pyrophosphatase/phosphodiesterase reactions (2008), Electrophoresis, 29, 3685-3693.
    View publication on PubMed
Search for more literature for 3.6.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in COS-7 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Reactive blue 2
-
 Homo sapiens
suramin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
 ATP E-NPP1, in 10 mM HEPES, 1 mM MgCl2, and 2 mM CaCl2, pH 7.4, at 37°C Homo sapiens
0.0074
-
 ATP E-NPP3, in 10 mM HEPES, 1 mM MgCl2, and 2 mM CaCl2, pH 7.4, at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
ATP + H2O best substrate Homo sapiens diphosphate + AMP
-
 ?
UTP + H2O
-
 Homo sapiens diphosphate + UMP
-
 ?

Synonyms

Synonyms Comment Organism
E-NPP
-
 Homo sapiens
ecto-nucleotide pyrophosphatase/phosphodiesterase
-
 Homo sapiens
NPP1 also named PC-1 Homo sapiens
NPP3 also named gp130RB13-6, B10, PDIbeta Homo sapiens
nucleotide pyrophosphatase/phosphodiesterase
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00004
-
 suramin E-NPP3, in 10 mM HEPES, 1 mM MgCl2, and 2 mM CaCl2, pH 7.4, at 37°C Homo sapiens
0.00026
-
 suramin E-NPP1, in 10 mM HEPES, 1 mM MgCl2, and 2 mM CaCl2, pH 7.4, at 37°C Homo sapiens
0.00052
-
 Reactive blue 2 E-NPP1, in 10 mM HEPES, 1 mM MgCl2, and 2 mM CaCl2, pH 7.4, at 37°C Homo sapiens
0.00071
-
 Reactive blue 2 E-NPP3, in 10 mM HEPES, 1 mM MgCl2, and 2 mM CaCl2, pH 7.4, at 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0012
-
 E-NPP3, in 10 mM HEPES, 1 mM MgCl2, and 2 mM CaCl2, pH 7.4, at 37°C Homo sapiens suramin
0.002
-
 E-NPP3, in 10 mM HEPES, 1 mM MgCl2, and 2 mM CaCl2, pH 7.4, at 37°C Homo sapiens Reactive blue 2
0.0086
-
 E-NPP1, in 10 mM HEPES, 1 mM MgCl2, and 2 mM CaCl2, pH 7.4, at 37°C Homo sapiens suramin
0.017
-
 E-NPP1, in 10 mM HEPES, 1 mM MgCl2, and 2 mM CaCl2, pH 7.4, at 37°C Homo sapiens Reactive blue 2