Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha,beta-methylene adenosine 5'-triphosphate | competitive inhibition, Ki: 25 mM | Rattus sp. | |
AMP-amidate | competitive inhibition, Ki: 30 mM | Rattus sp. | |
EDTA | complete inactivation, partially restored by Mg2+, Ca2+ | Rattus sp. | |
UDP-glucose | competitive inhibition, Ki: 18 mM | Rattus sp. | |
UMP | competitive inhibition, Ki: 26 mM | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
thymidine 5'-monophosphate p-nitrophenyl ester | - |
Rattus sp. | |
33 | - |
UTP | - |
Rattus sp. | |
50 | - |
UDP-glucose | - |
Rattus sp. | |
78 | - |
ATP | - |
Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Rattus sp. | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for optimal activity | Rattus sp. | |
Mg2+ | required for optimal activity | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | Rattus sp. | low activity | AMP + phosphate | - |
? | |
ATP + H2O | Rattus sp. | - |
AMP + diphosphate | - |
? | |
DNA + H2O | Rattus sp. | E. coli, low activity | adenosine 5'-monophosphate + ? | - |
? | |
polyadenylate + H2O | Rattus sp. | low activity | adenosine 5'-monophosphate + ? | - |
? | |
RNA + H2O | Rattus sp. | yeast, low activity | adenosine 5'-monophosphate + ? | - |
? | |
UDP + H2O | Rattus sp. | - |
UMP + phosphate | low activity | ? | |
UDP-glucose + H2O | Rattus sp. | - |
UMP + D-glucose-1-phosphate | - |
? | |
UTP + H2O | Rattus sp. | - |
UMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography rechniques | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
151 | - |
- |
Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | low activity | Rattus sp. | AMP + phosphate | - |
? | |
ATP + H2O | - |
Rattus sp. | AMP + diphosphate | - |
? | |
DNA + H2O | E. coli, low activity | Rattus sp. | adenosine 5'-monophosphate + ? | - |
? | |
polyadenylate + H2O | low activity | Rattus sp. | adenosine 5'-monophosphate + ? | - |
? | |
RNA + H2O | yeast, low activity | Rattus sp. | adenosine 5'-monophosphate + ? | - |
? | |
thymidine 5'-monophosphate p-nitrophenyl ester + H2O | significant phosphodiesterase activity | Rattus sp. | thymidine 5'-monophosphate + p-nitrophenol | - |
? | |
UDP + H2O | - |
Rattus sp. | UMP + phosphate | low activity | ? | |
UDP-glucose + H2O | - |
Rattus sp. | UMP + D-glucose-1-phosphate | - |
? | |
UTP + H2O | - |
Rattus sp. | UMP + diphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Rattus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
instability below | Rattus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
alpha,beta-methylene adenosine 5'-triphosphate | - |
Rattus sp. |