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Literature summary for 3.6.1.9 extracted from

  • Haroz, R.K.; Twu, J.S.; Bretthauer, R.K.
    Purification and properties of a yeast nucleotide pyrophosphatase (1972), J. Biol. Chem., 247, 1452-1457.
    View publication on PubMed
Search for more literature for 3.6.1.9

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 90% inhibition at 16 mM Saccharomyces sp.
dithiothreitol complete inactivation at 4 mM Saccharomyces sp.
EDTA complete inhibition at 2 mM, partial reactivation by divalent cations Saccharomyces sp.
GDP-mannose competitive inhibition of phosphodiesterase activity Saccharomyces sp.
L-cysteine complete inactivation at 4 mM Saccharomyces sp.
NAD+ competitive inhibition of phosphodiesterase activity Saccharomyces sp.
o-phenanthroline complete inactivation at 2 mM, partial reactivation by divalent cations Saccharomyces sp.
Zn2+ above 1-2 mM of EDTA-concentration optimal in restorage, greater values inhibitory Saccharomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 NAD+
-
 Saccharomyces sp.
1
-
 GDP-mannose
-
 Saccharomyces sp.
3.3
-
 UDP-glucose
-
 Saccharomyces sp.
5.6
-
 thymidine 5'-monophosphate p-nitrophenyl ester
-
 Saccharomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Saccharomyces sp.
65000
-
 1 * 65000, SDS-PAGE Saccharomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-glucose + H2O Saccharomyces sp.
-
 AMP + alpha-D-glucose 1-phosphate
-
 ?
CDP-glucose + H2O Saccharomyces sp.
-
 CMP + D-glucose-1-phosphate
-
 ?
GDP-mannose + H2O Saccharomyces sp.
-
 GMP + D-mannose-1-phosphate
-
 ?
NAD+ + H2O Saccharomyces sp.
-
 NMN + AMP
-
 ?
NADH + H2O Saccharomyces sp.
-
 NMNH + AMP
-
 ?
NADP+ + H2O Saccharomyces sp.
-
 NMN + adenosine 2',5'-phosphate
-
 ?
TDP-glucose + H2O Saccharomyces sp.
-
 TMP + D-glucose-1-phosphate
-
 ?
UDP-glucose + H2O Saccharomyces sp.
-
 UMP + D-glucose-1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces sp.
-
 hybrid of Saccharomyces fragilis x Saccharomyces dobzhanskii
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Saccharomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Saccharomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
640
-
-
 Saccharomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + H2O
-
 Saccharomyces sp. AMP + alpha-D-glucose 1-phosphate
-
 ?
ADP-glucose + H2O
-
 Saccharomyces sp. AMP + alpha-D-glucose-1-phosphate
-
 ?
bis(p-nitrophenyl)phosphate + H2O low activity Saccharomyces sp. p-nitrophenol + p-nitrophenylphosphate
-
 ?
CDP-glucose + H2O
-
 Saccharomyces sp. CMP + D-glucose-1-phosphate
-
 ?
GDP-mannose + H2O
-
 Saccharomyces sp. GMP + D-mannose-1-phosphate
-
 ?
NAD+ + H2O
-
 Saccharomyces sp. NMN + AMP
-
 ?
NADH + H2O
-
 Saccharomyces sp. NMNH + AMP
-
 ?
NADP+ + H2O
-
 Saccharomyces sp. NMN + adenosine 2',5'-phosphate
-
 ?
TDP-glucose + H2O
-
 Saccharomyces sp. TMP + D-glucose-1-phosphate
-
 ?
thymidine 5'-monophosphate p-nitrophenyl ester + H2O very low activity Saccharomyces sp. thymidine 5'-monophosphate + p-nitrophenol
-
 ?
UDP-glucose + H2O
-
 Saccharomyces sp. UMP + D-glucose-1-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Saccharomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6 8.7
-
 Saccharomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
8.2
-
 half-maximal activity Saccharomyces sp.