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Literature summary for 3.6.1.9 extracted from
- Novel activity of potato nucleotide pyrophosphatase (1976), Biochim. Biophys. Acta, 438, 540-550.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-AMP | effective inhibitor, Ki: 0.5 mM | Solanum tuberosum |  |
| adenosine 3'-monophosphate | Ki: 1 mM | Solanum tuberosum | |
| Cu2+ | 45% inhibition at 1 mM | Solanum tuberosum | |
| Hg2+ | 25% inhibition at 1 mM | Solanum tuberosum | |
| phosphate | Ki: 4 mM | Solanum tuberosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
thymidine 5'-monophosphate p-nitrophenyl ester | - |
Solanum tuberosum | |
| 1.2 | - |
p-nitrophenylphosphate | - |
Solanum tuberosum | |
| 1.3 | - |
thymidine 3'-monophosphate p-nitrophenyl ester | - |
Solanum tuberosum | |
| 1.3 | - |
bis(p-nitrophenylphosphate) | - |
Solanum tuberosum | |
| 2.5 | - |
thymidine 5'-alpha-naphthylphosphate | - |
Solanum tuberosum | |
| 2.9 | - |
thymidine 3'-alpha-naphthylphosphate | - |
Solanum tuberosum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | Solanum tuberosum | - |
AMP + phosphate | - |
? | |
| ATP + H2O | Solanum tuberosum | - |
AMP + diphosphate | - |
? | |
| mRNA + H2O | Solanum tuberosum | - |
7-methyl-GMP + ? | - |
? | |
| NAD+ + H2O | Solanum tuberosum | best natural substrate tested | NMN + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
potato | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| near homogeneity | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 50.3 | - |
- |
Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine 3'-(alpha-naphthylphosphate) | very low activity | Solanum tuberosum | adenosine + alpha-naphthylphosphate | - |
? | |
| ADP + H2O | - |
Solanum tuberosum | AMP + phosphate | - |
? | |
| alpha-naphthylphosphate + H2O | low activity | Solanum tuberosum | alpha-naphthol + phosphate | - |
? | |
| ATP + H2O | - |
Solanum tuberosum | AMP + diphosphate | - |
? | |
| bis(p-nitrophenyl)phosphate + H2O | - |
Solanum tuberosum | p-nitrophenol + p-nitrophenylphosphate | - |
? | |
| bis-alpha-naphthylphosphate + H2O | very low activity | Solanum tuberosum | ? | - |
? | |
| mRNA + H2O | - |
Solanum tuberosum | 7-methyl-GMP + ? | - |
? | |
| NAD+ + H2O | best natural substrate tested | Solanum tuberosum | NMN + AMP | - |
? | |
| p-nitrophenylphosphate + H2O | - |
Solanum tuberosum | p-nitrophenol + phosphate | - |
? | |
| thymidine + alpha-naphthylphosphate + H2O | very low activity | Solanum tuberosum | thymidine 3'-alpha-naphthol + phosphate | - |
? | |
| thymidine 3'-alpha-naphthylphosphate + H2O | - |
Solanum tuberosum | thymidine 3'-alpha-naphthol + phosphate | - |
? | |
| thymidine 3'-diphospho 3'-thymidine + H2O | - |
Solanum tuberosum | thymidine 3'-phosphate + thymidine 3'-phosphate | - |
? | |
| thymidine 3'-monophosphate p-nitrophenyl ester + H2O | - |
Solanum tuberosum | thymidine 3'-monophosphate + p-nitrophenol | - |
? | |
| thymidine 5'-alpha-naphthylphosphate + H2O | very low activity | Solanum tuberosum | thymidine + alpha-naphthylphosphate | - |
? | |
| thymidine 5'-monophosphate p-nitrophenyl ester + H2O | - |
Solanum tuberosum | thymidine 5'-monophosphate + p-nitrophenol | - |
? | |
| uridine 3'-(alpha-naphthylphosphate) + H2O | very low activity | Solanum tuberosum | uridine + alpha-naphthylphosphate | - |
? | |
| uridine 3'-(phenylphosphate) + H2O | very low activity | Solanum tuberosum | uridine + phenylphosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
55% loss of activity in 5 min | Solanum tuberosum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | optimal activity within | Solanum tuberosum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
adenosine 3'-monophosphate | - |
Solanum tuberosum | |
| 4 | - |
phosphate | - |
Solanum tuberosum | |
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