General Stability | Organism |
---|---|
tetrameric form stored at 4°C for longer periods remains active, but in aqueous solution higher insoluble aggregates formed | Solanum tuberosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
4 * 74000, SDS-PAGE | Solanum tuberosum |
74000 | - |
2 * 74000, SDS-PAGE, alkali-treated preparation, different substrate specificity compared to the tetrameric form | Solanum tuberosum |
346000 | - |
sucrose density gradient centrifugation | Solanum tuberosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cAMP + H2O | Solanum tuberosum | low activity for the tetramer | adenosine monophosphate | - |
? | |
NAD+ + H2O | Solanum tuberosum | tetramer 4fold more active than dimer | NMN + AMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | - |
potato | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tuber | - |
Solanum tuberosum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cAMP + H2O | low activity for the tetramer | Solanum tuberosum | adenosine monophosphate | - |
? | |
bis(p-nitrophenyl)phosphate + H2O | dimer nearly 4fold more active than tetramer | Solanum tuberosum | p-nitrophenol + p-nitrophenylphosphate | - |
? | |
NAD+ + H2O | tetramer 4fold more active than dimer | Solanum tuberosum | NMN + AMP | - |
? | |
p-nitrophenyl phenylphosphonate + H2O | dimer 8fold more active than tetramer | Solanum tuberosum | p-nitrophenol + phenylphosphonate | - |
? | |
P1,P2-di(adenosine-5') diphosphate + H2O | tetramer 3fold more active than dimer | Solanum tuberosum | ? | - |
? | |
P1,P2-di(thymidine-3') diphosphate + H2O | very low activity for both dimer and tetramer | Solanum tuberosum | ? | - |
? | |
P1,P2-di(thymidine-5') diphosphate + H2O | low activity for both dimer and tetramer | Solanum tuberosum | ? | - |
? | |
P1,P3-di(adenosine-5') triphosphate + H2O | tetramer 2fold more active than dimer | Solanum tuberosum | ? | - |
? | |
thymidine 3'-monophosphate p-nitrophenyl ester + H2O | dimer 10fold more active than tetramer | Solanum tuberosum | thymidine 3'-monophosphate + p-nitrophenol | - |
? | |
thymidine 5'-monophosphate p-nitrophenyl ester + H2O | equal activity for dimer and tetramer | Solanum tuberosum | thymidine 5'-monophosphate + p-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 74000, SDS-PAGE, alkali-treated preparation, different substrate specificity compared to the tetrameric form | Solanum tuberosum |
tetramer | 4 * 74000, SDS-PAGE | Solanum tuberosum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
rapid loss of activity for the dimeric form, tetrameric form remains fully stable | Solanum tuberosum |
55 | - |
full stability for the tetramer up to 1 h | Solanum tuberosum |