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Literature summary for 3.6.1.9 extracted from
- Characterization of H. parasuis periplasmic nucleotide pyrophosphatase as a potential target enzyme for inhibition of growth (1997), Vet. Microbiol., 58, 261-276.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-ADP | competitive inhibition, Ki: 0.0019 mM | Glaesserella parasuis |  |
| 5'-AMP | competitive inhibition, Ki: 0.037 mM | Glaesserella parasuis | |
| cytidine 5'-diphosphate | competitive inhibition, Ki: 0.0085 mM | Glaesserella parasuis | |
| GDP | competitive inhibition, Ki: 0.0025 mM | Glaesserella parasuis | |
| N1-butylnicotinamide chloride | competitive inhibition, Ki: 0.408 mM | Glaesserella parasuis | |
| N1-heptylnicotinamide chloride | competitive inhibition, Ki: 0.48 mM | Glaesserella parasuis | |
| N1-hexylnicotinamide chloride | competitive inhibition, Ki: 0.629 mM | Glaesserella parasuis | |
| N1-octylnicotinamide chloride | competitive inhibition, Ki: 0.287 mM | Glaesserella parasuis | |
| N1-pentylnicotinamide chloride | competitive inhibition, Ki: 0.297 mM | Glaesserella parasuis | |
| uridine 5'-diphosphate | competitive inhibition, Ki: 0.0012 mM | Glaesserella parasuis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
nicotinamide guanine dinucleotide | - |
Glaesserella parasuis | |
| 0.004 | - |
NAD+ | biphasic hydrolysis, functions in a manner indicative of negative cooperativity | Glaesserella parasuis | |
| 0.005 | - |
NADP+ | - |
Glaesserella parasuis | |
| 0.015 | - |
FAD | does not exhibit negative cooperativity | Glaesserella parasuis | |
| 0.144 | - |
nicotinamide 1,N6-ethenoadenine dinucleotide | does not exhibit negative cooperativity | Glaesserella parasuis | |
| 0.351 | - |
coenzyme A | does not exhibit negative cooperativity | Glaesserella parasuis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Glaesserella parasuis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Glaesserella parasuis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-ribose + H2O | Glaesserella parasuis | - |
AMP + phosphoribose | - |
? | |
| coenzyme A + H2O | Glaesserella parasuis | low activity | ? | - |
? | |
| FAD + H2O | Glaesserella parasuis | - |
FMN + AMP | - |
? | |
| NAD+ + H2O | Glaesserella parasuis | - |
NMN + AMP | - |
? | |
| NADP+ + H2O | Glaesserella parasuis | - |
NMN + adenosine 2',5'-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glaesserella parasuis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques | Glaesserella parasuis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | potential growth inhibition by adenosine diphosphate | Glaesserella parasuis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.5 | - |
- |
Glaesserella parasuis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 0.1 M KCl, 6 months | Glaesserella parasuis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-dihydronicotinamide adenine dinucleotide + H2O | - |
Glaesserella parasuis | 1,4-dihydronicotinamide mononucleotide + 5'-AMP | - |
? | |
| 3-acetylpyridine adenine dinucleotide + H2O | - |
Glaesserella parasuis | 3-acetylpyridine riboside 5'-phosphate + 5'-AMP | - |
? | |
| 3-aminopyridine adenine dinucleotide + H2O | - |
Glaesserella parasuis | 3-aminopyridine riboside 5'-phosphate + 5'-AMP | - |
? | |
| 3-methylpyridine adenine dinucleotide + H2O | - |
Glaesserella parasuis | 3-methylpyridine mononucleotide + 5'-AMP | - |
? | |
| 3-pyridylcarbinol adenine dinucleotide + H2O | - |
Glaesserella parasuis | ? | - |
? | |
| ADP-ribose + H2O | - |
Glaesserella parasuis | AMP + phosphoribose | - |
? | |
| coenzyme A + H2O | low activity | Glaesserella parasuis | ? | - |
? | |
| FAD + H2O | - |
Glaesserella parasuis | FMN + AMP | - |
? | |
| GDP-glucose + H2O | - |
Glaesserella parasuis | GMP + D-glucose-1-phosphate | - |
? | |
| NAD+ + H2O | - |
Glaesserella parasuis | NMN + AMP | - |
? | |
| NADP+ + H2O | - |
Glaesserella parasuis | NMN + adenosine 2',5'-phosphate | - |
? | |
| nicotinamide 1,N6-ethenoadenine dinucleotide + H2O | - |
Glaesserella parasuis | NMN + N6-ethenoadenosine 5'-phosphate | - |
? | |
| nicotinamide guanine dinucleotide + H2O | - |
Glaesserella parasuis | NMN + GMP | - |
? | |
| nicotinamide hypoxanthine dinucleotide + H2O | - |
Glaesserella parasuis | NMN + hypoxanthine monophosphate | - |
? | |
| nicotinic acid adenine dinucleotide + H2O | - |
Glaesserella parasuis | nicotinic acid mononucleotide + 5'-AMP | - |
? | |
| pyridine adenine dinucleotide + H2O | - |
Glaesserella parasuis | pyridine riboside 5'-phosphate + 5'-AMP | - |
? | |
| thionicotinamide adenine dinucleotide + H2O | - |
Glaesserella parasuis | thionicotinamide mononucleotide + 5'-AMP | - |
? | |
| uridine diphosphoglucose + H2O | - |
Glaesserella parasuis | UMP + D-glucose-1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Glaesserella parasuis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
below no thermal inactivation within 60 min | Glaesserella parasuis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
uridine 5'-diphosphate | competitive inhibition | Glaesserella parasuis | |
| 0.0025 | - |
GDP | competitive inhibition | Glaesserella parasuis | |
| 0.0085 | - |
cytidine 5'-diphosphate | competitive inhibition | Glaesserella parasuis | |
| 0.287 | - |
N1-octylnicotinamide chloride | competitive inhibition | Glaesserella parasuis | |
| 0.297 | - |
N1-pentylnicotinamide chloride | competitive inhibition | Glaesserella parasuis | |
| 0.408 | - |
N1-butylnicotinamide chloride | competitive inhibition | Glaesserella parasuis | |
| 0.48 | - |
N1-heptylnicotinamide chloride | competitive inhibition | Glaesserella parasuis | |
| 0.629 | - |
N1-hexylnicotinamide chloride | competitive inhibition | Glaesserella parasuis | |
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