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Literature summary for 3.6.1.9 extracted from

  • Medda, R.; Padiglia, A.; Lorrai, A.; Murgia, B.; Agro, A.F.; Castagnola, M.; Floris, G.
    Purification and properties of a nucleotide pyrophosphatase from lentil seddlings (2000), J. Protein Chem., 19, 209-214.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 50% inhibition at 1.5 mM Lens culinaris
diethyldiphosphate inactivation follows apparent first-order kinetics, completely restored by hydroxylamine Lens culinaris
EDTA 50% inhibition at 0.2 mM Lens culinaris
Ni2+ 50% inhibition at 2 mM Lens culinaris
Zn2+ 50% inhibition at 20 mM Lens culinaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
NADH
-
Lens culinaris
0.043
-
NADP+
-
Lens culinaris
0.047
-
NADPH
-
Lens culinaris
0.049
-
NAD+
-
Lens culinaris
0.066
-
thymidine 5'-monophosphate p-nitrophenyl ester
-
Lens culinaris

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Lens culinaris
Mg2+ activation Lens culinaris
Mn2+ 100% activation at 20 mM Lens culinaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
gel filtration Lens culinaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 diadenosine 5',5''-P1,P4-tetraphosphate + 3 H2O Lens culinaris
-
2 ATP + 2 ADP + 2 AMP
-
?
ADP-ribose + H2O Lens culinaris low activity AMP + phosphoribose
-
?
alpha-NADH + H2O Lens culinaris low activity alpha-NMNH + AMP
-
?
diadenosine 5',5''-P1, P2-diphosphate + H2O Lens culinaris
-
AMP + AMP
-
?
diadenosine 5',5''-P1,P3-triphosphate + H2O Lens culinaris
-
AMP + ADP
-
?
diadenosine 5',5''-P1,P5-pentaposphate + H2O Lens culinaris
-
ATP + ADP + AMP + adenosine 5'-tetraphosphate
-
?
FAD + H2O Lens culinaris low activity FMN + AMP
-
?
NAD+ + H2O Lens culinaris
-
NMN + AMP
-
?
NADH + H2O Lens culinaris best natural substrate, reduced form more rapidly hydrolysed than oxidized form NMNH + AMP
-
?
NADP+ + H2O Lens culinaris
-
NMN + adenosine 2',5'-phosphate
-
?
NADPH + H2O Lens culinaris reduced form more rapidly hydrolysed than oxidized form NMNH + adenosine 2',5'-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Lens culinaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Lens culinaris

Reaction

Reaction Comment Organism Reaction ID
a nucleoside triphosphate + H2O = a nucleotide + diphosphate substrates include NAD+, NADP+, FAD, CoA and also ATP and ADP Lens culinaris

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Lens culinaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.9
-
-
Lens culinaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 diadenosine 5',5''-P1,P4-tetraphosphate + 3 H2O
-
Lens culinaris 2 ATP + 2 ADP + 2 AMP
-
?
ADP-ribose + H2O low activity Lens culinaris AMP + phosphoribose
-
?
alpha-NADH + H2O low activity Lens culinaris alpha-NMNH + AMP
-
?
diadenosine 5',5''-P1, P2-diphosphate + H2O
-
Lens culinaris AMP + AMP
-
?
diadenosine 5',5''-P1,P3-triphosphate + H2O
-
Lens culinaris AMP + ADP
-
?
diadenosine 5',5''-P1,P5-pentaposphate + H2O
-
Lens culinaris ATP + ADP + AMP + adenosine 5'-tetraphosphate
-
?
FAD + H2O low activity Lens culinaris FMN + AMP
-
?
NAD+ + H2O
-
Lens culinaris NMN + AMP
-
?
NADH + H2O best natural substrate, reduced form more rapidly hydrolysed than oxidized form Lens culinaris NMNH + AMP
-
?
NADP+ + H2O
-
Lens culinaris NMN + adenosine 2',5'-phosphate
-
?
NADPH + H2O reduced form more rapidly hydrolysed than oxidized form Lens culinaris NMNH + adenosine 2',5'-phosphate
-
?
P1-5'-(7-methyl)-guanosine-P3-5'-guanosine triphosphate + H2O cap analogue Lens culinaris 7-methylguanosine 5'-diphosphate + GMP
-
?
thymidine 5'-monophosphate p-nitrophenyl ester + H2O faster cleavage than other substrates tested Lens culinaris thymidine 5'-monophosphate + p-nitrophenol
-
?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Lens culinaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Lens culinaris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
EDTA
-
Lens culinaris
1.5
-
Cu2+
-
Lens culinaris
2
-
Ni2+
-
Lens culinaris
20
-
Zn2+
-
Lens culinaris