Literature summary for 3.6.1.75 extracted from

Wu, W.I.; Liu, Y.; Riedel, B.; Wissing, J.B.; Fischl, A.S.; Carman, G.M.
Purification and characterization of diacylglycerol pyrophosphate phosphatase from Saccharomyces cerevisiae (1996), J. Biol. Chem., 271, 1868-1876.

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Activating Compound

Activating Compound	Comment	Organism	Structure
phosphatidylethanolamine	-	Saccharomyces cerevisiae
phosphatidylinositol	-	Saccharomyces cerevisiae
Triton X-100	addition of Triton X-100 stimulates DGPP phosphatase activity to a maximum at a concentration of 2 mM	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
4-chloromercuriphenylsulfonic acid	41% inhibition at 1 mM	Saccharomyces cerevisiae
ADP	24% inhibition at a 10fold excess of inhibitor to substrate diacylglycerol diphosphate	Saccharomyces cerevisiae
Ca2+	-	Saccharomyces cerevisiae
diacylglycerol	slight inhibition	Saccharomyces cerevisiae
diphosphate	48% inhibition at a 10fold excess of inhibitor to substrate diacylglycerol diphosphate	Saccharomyces cerevisiae
Mg2+	-	Saccharomyces cerevisiae
Mn2+	-	Saccharomyces cerevisiae
additional information	poor inhibition by ATP and AMP, the enzyme is inhibited by divalent cations, but is relatively insensitive to thioreactive agents, no inhibition by phospholipids or phosphatidic acid versus diacylglycerol diphosphate as substrate, no inhibition by EDTA, glycerol 3-phosphate, and inositol 1-phosphate	Saccharomyces cerevisiae
NaF	94% inhibition at 10 mM	Saccharomyces cerevisiae
NEM	10% inhibition at 5 mM	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics, the DGPP phosphatase exhibits typical saturation kinetics with respect to DGPP, the Km value for diacylglycerol diphosphate is 3fold greater than its cellular concentration of 0.18 mol%	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	associated	Saccharomyces cerevisiae	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	x * 34000, SDS-PAGE	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
diacylglycerol diphosphate + H2O	Saccharomyces cerevisiae	preferred substrate	phosphatidate + phosphate	-	?
diacylglycerol diphosphate + H2O	Saccharomyces cerevisiae MATa ade5	preferred substrate	phosphatidate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-
Saccharomyces cerevisiae MATa ade5	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
33333fold to homogeneity by solubilization from membranes with Triton X-100, followed by anion exchange chromatography, affinity and hydroxylapatite chromatography, and again anion exchange chromatography	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
150	-	purified enzyme	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diacylglycerol diphosphate + H2O	preferred substrate	Saccharomyces cerevisiae	phosphatidate + phosphate	-	?
diacylglycerol diphosphate + H2O	preferred substrate, the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it also removes the phosphate from phosphatidate to form diacylglycerol, reaction of EC 3.1.3.4	Saccharomyces cerevisiae	phosphatidate + phosphate	product identification by TLC	?
diacylglycerol diphosphate + H2O	preferred substrate	Saccharomyces cerevisiae MATa ade5	phosphatidate + phosphate	-	?
diacylglycerol diphosphate + H2O	preferred substrate, the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it also removes the phosphate from phosphatidate to form diacylglycerol, reaction of EC 3.1.3.4	Saccharomyces cerevisiae MATa ade5	phosphatidate + phosphate	product identification by TLC	?

Subunits

Subunits	Comment	Organism
?	x * 34000, SDS-PAGE	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
DGPP phosphatase	-	Saccharomyces cerevisiae
diacylglycerol pyrophosphate phosphatase	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
40.9	-	diacylglycerol diphosphate	pH 6.5, 30°C	Saccharomyces cerevisiae
96.67	-	diacylglycerol diphosphate	pH 6.5, 30°C	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	8.5	-	Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	-	Saccharomyces cerevisiae
0.058	-	Mn2+	pH 6.5, 30°C	Saccharomyces cerevisiae
0.56	-	Ca2+	pH 6.5, 30°C	Saccharomyces cerevisiae
17	-	Mg2+	pH 6.5, 30°C	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)