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Literature summary for 3.6.1.74 extracted from

  • Itoh, N.; Mizumoto, K.; Kaziro, Y.
    Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. II. Catalytic properties (1984), J. Biol. Chem., 259, 13930-13936 .
    View publication on PubMed
Search for more literature for 3.6.1.74

Inhibitors

Inhibitors Comment Organism Structure
GTP 60% inhibition at 1 mM Saccharomyces cerevisiae
Mn2+ 90% inhibition at 1 mM Saccharomyces cerevisiae
additional information no inhibition by GDP and ppGp Saccharomyces cerevisiae
phosphate 31% inhibition at 10 mM, 91% at 30 mM Saccharomyces cerevisiae
polyA 12% inhibition at 0.1 mg/ml Saccharomyces cerevisiae
polyU 88% inhibition at 0.1 mg/ml Saccharomyces cerevisiae
ppGpCpC 50% inhibition at about 0.05 mM Saccharomyces cerevisiae
ppGpCpC(A2,U2,G) 46% inhibition at 0.005 mM Saccharomyces cerevisiae
pppGp 50% inhibition at about 0.1 mM Saccharomyces cerevisiae
pppGpCpC 50% inhibition at about 0.005 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Saccharomyces cerevisiae
0.0014
-
 pppA-terminated poly(A) pH 7.0, 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimum concentrations between 0.03 and 0.5 mM Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 5'-triphospho-[mRNA] + H2O Saccharomyces cerevisiae
-
 a 5'-diphospho-[mRNA] + phosphate
-
 ?
a 5'-triphospho-[mRNA] + H2O Saccharomyces cerevisiae ATCC 204508
-
 a 5'-diphospho-[mRNA] + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae O13297
-
-
Saccharomyces cerevisiae ATCC 204508 O13297
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 5'-triphospho-[mRNA] + H2O
-
 Saccharomyces cerevisiae a 5'-diphospho-[mRNA] + phosphate
-
 ?
a 5'-triphospho-[mRNA] + H2O
-
 Saccharomyces cerevisiae ATCC 204508 a 5'-diphospho-[mRNA] + phosphate
-
 ?
additional information the enzyme is specific for the gamma-phosphoryl group at the 5'-terminus of RNA, it does not hydrolyze ATP. It can hydrolyze the gamma-phosphoryl group of pppGp, but the RNA substrates with longer chain length are preferred Saccharomyces cerevisiae ?
-
-
additional information the enzyme is specific for the gamma-phosphoryl group at the 5'-terminus of RNA, it does not hydrolyze ATP. It can hydrolyze the gamma-phosphoryl group of pppGp, but the RNA substrates with longer chain length are preferred Saccharomyces cerevisiae ATCC 204508 ?
-
-
pppA-terminated poly(A) + H2O
-
 Saccharomyces cerevisiae ppA-terminated poly(A) + phosphate
-
 ?
pppA-terminated poly(A) + H2O
-
 Saccharomyces cerevisiae ATCC 204508 ppA-terminated poly(A) + phosphate
-
 ?
pppG-terminated mRNA + H2O lower activity Saccharomyces cerevisiae ppG-terminated mRNA + phosphate
-
 ?
pppG-terminated mRNA + H2O lower activity Saccharomyces cerevisiae ATCC 204508 ppG-terminated mRNA + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
mRNA-capping enzyme
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5 broad optimum Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function the mRNA-capping enzyme from Saccharomyces cerevisiae catalyzes (a) removal of the gamma-phosphoryl group from the 5'-end of the newly formed mRNA and (b) guanylylation of the resulting diphosphoryl end (EC 2.7.7.50) Saccharomyces cerevisiae