Literature summary for 3.6.1.71 extracted from

Tumbale, P.; Appel, C.D.; Kraehenbuehl, R.; Robertson, P.D.; Williams, J.S.; Krahn, J.; Ahel, I.; Williams, R.S.
Structure of an aprataxin-DNA complex with insights into AOA1 neurodegenerative disease (2011), Nat. Struct. Mol. Biol., 18, 1189-1195.

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of APTX in complex with DNA, AMP and Zn2+ reveals active site and DNA interaction clefts formed by fusing a histidine triad nucleotide hydrolase with an DNA minor groove binding C2HE Zn-finger. An Aptx helical wedge interrogates the base stack for DNA end/nick sensing. Histidine triad nucleotide hydrolase, Zn-finger, the wedge, and an [F/Y]PK pivot motif cooperate to distort terminal DNA base-pairing and direct 5'-AMP into the active site pocket	Schizosaccharomyces pombe

Crystallization (Comment)

Organism

structure of APTX in complex with DNA, AMP and Zn2+ reveals active site and DNA interaction clefts formed by fusing a histidine triad nucleotide hydrolase with an DNA minor groove binding C2HE Zn-finger. An Aptx helical wedge interrogates the base stack for DNA end/nick sensing. Histidine triad nucleotide hydrolase, Zn-finger, the wedge, and an [F/Y]PK pivot motif cooperate to distort terminal DNA base-pairing and direct 5'-AMP into the active site pocket

Schizosaccharomyces pombe

Protein Variants

Protein Variants	Comment	Organism
H138A	mutation significantly impairs deadenylation activity	Schizosaccharomyces pombe
H147N	complete loss of activity	Schizosaccharomyces pombe
S168A	mutation significantly impairs deadenylation activity	Schizosaccharomyces pombe

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	O74859	-	-
Schizosaccharomyces pombe ATCC 24843	O74859	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
adenosine-5'-diphospho-5'-[DNA] + H2O	-	Schizosaccharomyces pombe	AMP + phospho-5'-[DNA]	-	?
adenosine-5'-diphospho-5'-[DNA] + H2O	-	Schizosaccharomyces pombe ATCC 24843	AMP + phospho-5'-[DNA]	-	?
additional information	mechanism involves nucleophilic attack of DNA 5'-adenylate by His147 to generate a covalent enzyme-AMP intermediate. His147 is stabilized by a close hydrogen bond with the Asn145 main chain carbonyl. The Aptx-specific His168-Ser168 pair appears positioned to activate solvent for hydrolysis of the enzyme-AMP intermediate. His168 may also act as a proton donor to the bridging oxygen in the first step	Schizosaccharomyces pombe	?	-	?
additional information	mechanism involves nucleophilic attack of DNA 5'-adenylate by His147 to generate a covalent enzyme-AMP intermediate. His147 is stabilized by a close hydrogen bond with the Asn145 main chain carbonyl. The Aptx-specific His168-Ser168 pair appears positioned to activate solvent for hydrolysis of the enzyme-AMP intermediate. His168 may also act as a proton donor to the bridging oxygen in the first step	Schizosaccharomyces pombe ATCC 24843	?	-	?

Synonyms

Synonyms	Comment	Organism
aprataxin-like protein	-	Schizosaccharomyces pombe
HNT3	-	Schizosaccharomyces pombe