Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
expressed in Escherichia coli strain C41 | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with sodium formate as precipitant | Pyrococcus horikoshii |
sitting-drop vapour-diffusion method using sodium formate as precipitant at 16°C. The crystals belong to space group P3(2)21, with unit-cell parameters a = b = 85.65 A, c = 75.51 A. The asymmetric unit contains two molecules of enzyme | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
HiTrap SP HP column chromatography and Superdex 75 column chromatography | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-diphosphoglycerate + H2O | - |
Pyrococcus horikoshii | 3-phosphoglycerate + phosphate | - |
? | |
acetyl phosphate + H2O | - |
Pyrococcus horikoshii | acetate + phosphate | - |
? | |
carbamoyl phosphate + H2O | - |
Pyrococcus horikoshii | carbamate + phosphate | - |
? | |
succinyl phosphate + H2O | - |
Pyrococcus horikoshii | succinate + phosphate | - |
? |