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Literature summary for 3.6.1.7 extracted from

  • Chiarugi, P.; Raugei, G.; Fiaschi, T.; Taddei, L.; Camici, G.; Ramponi, G.
    Characterization of a novel nucleolytic activity of acylphosphatases (1996), Biochem. Mol. Biol. Int., 40, 73-81.
    View publication on PubMed
Search for more literature for 3.6.1.7

Cloned(Commentary)

Cloned (Comment) Organism
muscle type and common type isoenzymes, expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
R23Q mutant of muscle type enzyme is totally inactive using benzoylphosphate as a substrate and not able to bind the phosphate moiety of that substrate Homo sapiens
R23Q mutant inactive on DNA, Arg23 possible has a central role for muscle type acylphosphatase activity on DNA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibition of ribonucleolytic and deoxyribonucleolytic activity of acylphosphatase Bos taurus
EDTA 1 mM: 100% inhibition of endonucleolytic activity of both isoforms; inhibition of ribonucleolytic and deoxyribonucleolytic activity of acylphosphatase Homo sapiens
EDTA inhibition of ribonucleolytic and deoxyribonucleolytic activity of acylphosphatase Meleagris gallopavo
phosphate phosphate: inhibition of nucleolytic activity Bos taurus
phosphate phosphate, 1 mM: competitive inhibition of DNAase activity of muscle and common type acylphosphatase, no inhibition of ribonucleolytic activity; phosphate: inhibition of nucleolytic activity Homo sapiens
phosphate phosphate: inhibition of nucleolytic activity Meleagris gallopavo

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Meleagris gallopavo 5737
-
cytoplasm
-
 Homo sapiens 5737
-
cytoplasm
-
 Bos taurus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
divalent cations nucleolytic activity, depends on Homo sapiens
Mg2+ Mg2+ dependent deoxyribonuclease and ribonuclease activity, required for nucleolytic activity Meleagris gallopavo
Mg2+ Mg2+ dependent deoxyribonuclease and ribonuclease activity, required for nucleolytic activity Homo sapiens
Mg2+ Mg2+ dependent deoxyribonuclease and ribonuclease activity, required for nucleolytic activity Bos taurus
MgCl2 Mg2+ dependent deoxyribonuclease and ribonuclease activity, required for activity Meleagris gallopavo
MgCl2 Mg2+ dependent deoxyribonuclease and ribonuclease activity, required for activity Homo sapiens
MgCl2 Mg2+ dependent deoxyribonuclease and ribonuclease activity, required for activity Bos taurus
Mn2+ DNAase activity, able to completely substitute Mg2+, required for nucleolytic activity Homo sapiens
additional information Ca2+ has no activating effect on the nucleolytic activity of acylphosphatase Homo sapiens
Zn2+ DNAase activity, activation of nucleolytic activity, less effective compared with Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Meleagris gallopavo nucleolytic activity of muscle and common type isoform acylphosphatase on RNA and DNA is possible physiological activity of these isoenzymes in the cell ?
-
 ?
additional information Homo sapiens nucleolytic activity of muscle and common type isoform acylphosphatase on RNA and DNA is possible physiological activity of these isoenzymes in the cell ?
-
 ?
additional information Bos taurus nucleolytic activity of muscle and common type isoform acylphosphatase on RNA and DNA is possible physiological activity of these isoenzymes in the cell ?
-
 ?
additional information Meleagris gallopavo acylphosphatase is active as a nuclease at pH ranging from 5.5 to 6.8 suggests that enzymes could play some role in apoptotic mechanisms ?
-
 ?
additional information Homo sapiens acylphosphatase is active as a nuclease at pH ranging from 5.5 to 6.8 suggests that enzymes could play some role in apoptotic mechanisms ?
-
 ?
additional information Bos taurus acylphosphatase is active as a nuclease at pH ranging from 5.5 to 6.8 suggests that enzymes could play some role in apoptotic mechanisms ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 bovine
-
Bos taurus
-
 common type, CT, isoenzyme
-
Homo sapiens
-
 human
-
Homo sapiens
-
 recombinant and mutants, R23Q mutant
-
Meleagris gallopavo
-
 turkey
-
Meleagris gallopavo
-
 muscle type, MT, isoenzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant muscle type and common type isoenzymes Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
an acylphosphate + H2O = a carboxylate + phosphate reaction mechanism Homo sapiens
a
an acylphosphate + H2O = a carboxylate + phosphate reaction mechanism Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Homo sapiens
-
testis common type enzyme Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-diphosphoglycerate + H2O
-
 Meleagris gallopavo 3-phosphoglycerate + phosphate
-
 ?
1,3-diphosphoglycerate + H2O
-
 Homo sapiens 3-phosphoglycerate + phosphate
-
 ?
1,3-diphosphoglycerate + H2O
-
 Bos taurus 3-phosphoglycerate + phosphate
-
 ?
acylphosphate + H2O specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Meleagris gallopavo carboxylate + phosphate
-
 ?
acylphosphate + H2O specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Homo sapiens carboxylate + phosphate
-
 ?
acylphosphate + H2O specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Bos taurus carboxylate + phosphate
-
 ?
benzoyl phosphate + H2O
-
 Meleagris gallopavo benzoate + phosphate
-
 ?
benzoyl phosphate + H2O
-
 Homo sapiens benzoate + phosphate
-
 ?
benzoyl phosphate + H2O
-
 Bos taurus benzoate + phosphate
-
 ?
carbamoyl phosphate + H2O
-
 Meleagris gallopavo carbamate + phosphate
-
 ?
carbamoyl phosphate + H2O
-
 Homo sapiens carbamate + phosphate
-
 ?
carbamoyl phosphate + H2O
-
 Bos taurus carbamate + phosphate
-
 ?
DNA + H2O muscle type and common type hydrolyze double stranded DNA, high molecular weight human genomic DNA Homo sapiens ?
-
 ?
DNA + H2O DNAase activity, nucleolytic activity, exonucleolytic and endonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Meleagris gallopavo ?
-
 ?
DNA + H2O DNAase activity, nucleolytic activity, exonucleolytic and endonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Homo sapiens ?
-
 ?
DNA + H2O DNAase activity, nucleolytic activity, exonucleolytic and endonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Bos taurus ?
-
 ?
DNA + H2O endonucleolytic activity on plasmid DNA, pRcCMV plasmid DNA, closed circular plasmid DNA, exonucleolytic activity on linear plasmid DNA Homo sapiens ?
-
 ?
additional information specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Meleagris gallopavo ?
-
 ?
additional information specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Homo sapiens ?
-
 ?
additional information specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Bos taurus ?
-
 ?
additional information nucleolytic, ribonucleolytic and deoxyribonucleolytic, activity, exonucleolytic and endonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Meleagris gallopavo ?
-
 ?
additional information nucleolytic, ribonucleolytic and deoxyribonucleolytic, activity, exonucleolytic and endonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Homo sapiens ?
-
 ?
additional information nucleolytic, ribonucleolytic and deoxyribonucleolytic, activity, exonucleolytic and endonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Bos taurus ?
-
 ?
additional information nucleolytic activity of muscle and common type isoform acylphosphatase on RNA and DNA is possible physiological activity of these isoenzymes in the cell Meleagris gallopavo ?
-
 ?
additional information nucleolytic activity of muscle and common type isoform acylphosphatase on RNA and DNA is possible physiological activity of these isoenzymes in the cell Homo sapiens ?
-
 ?
additional information nucleolytic activity of muscle and common type isoform acylphosphatase on RNA and DNA is possible physiological activity of these isoenzymes in the cell Bos taurus ?
-
 ?
additional information acylphosphatase is active as a nuclease at pH ranging from 5.5 to 6.8 suggests that enzymes could play some role in apoptotic mechanisms Meleagris gallopavo ?
-
 ?
additional information acylphosphatase is active as a nuclease at pH ranging from 5.5 to 6.8 suggests that enzymes could play some role in apoptotic mechanisms Homo sapiens ?
-
 ?
additional information acylphosphatase is active as a nuclease at pH ranging from 5.5 to 6.8 suggests that enzymes could play some role in apoptotic mechanisms Bos taurus ?
-
 ?
nucleic acids + H2O nucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Meleagris gallopavo ?
-
 ?
nucleic acids + H2O nucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Homo sapiens ?
-
 ?
nucleic acids + H2O nucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Bos taurus ?
-
 ?
poly (rA) + H2O ribonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Meleagris gallopavo ?
-
 ?
poly (rA) + H2O ribonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Homo sapiens ?
-
 ?
poly (rA) + H2O ribonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Bos taurus ?
-
 ?
RNA + H2O RNAase activity, ribonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Meleagris gallopavo ?
-
 ?
RNA + H2O RNAase activity, ribonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Homo sapiens ?
-
 ?
RNA + H2O RNAase activity, ribonucleolytic activity, in both muscle type, MT, and erythrocyte or common type, CT, isoenzymes Bos taurus ?
-
 ?
RNA + H2O Mg2+ dependent activity Meleagris gallopavo ?
-
 ?
RNA + H2O Mg2+ dependent activity Homo sapiens ?
-
 ?
RNA + H2O Mg2+ dependent activity Bos taurus ?
-
 ?
succinoyl phosphate + H2O
-
 Meleagris gallopavo succinate + phosphate
-
 ?
succinoyl phosphate + H2O
-
 Homo sapiens succinate + phosphate
-
 ?
succinoyl phosphate + H2O
-
 Bos taurus succinate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
More two isoenzymatic forms: one prevalent in skeletal and cardiac muscle named muscle type, the other in red blood cells named erythrocyte or common type, even if both isoforms are expressed in all tissues Meleagris gallopavo
More two isoenzymatic forms: one prevalent in skeletal and cardiac muscle named muscle type, the other in red blood cells named erythrocyte or common type, even if both isoforms are expressed in all tissues Homo sapiens
More two isoenzymatic forms: one prevalent in skeletal and cardiac muscle named muscle type, the other in red blood cells named erythrocyte or common type, even if both isoforms are expressed in all tissues Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 nucleolytic activity, assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 nucleolytic activity, assay at Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 Mg2+ dependent ribonucleolytic activity Homo sapiens
5 6.8 Mg2+ dependent deoxyribonuclease and ribonuclease activity Meleagris gallopavo
5 6.8 Mg2+ dependent deoxyribonuclease and ribonuclease activity Homo sapiens
5 6.8 Mg2+ dependent deoxyribonuclease and ribonuclease activity Bos taurus
7
-
 Mg2+ dependent ribonucleolytic and deoxyribonucleolytic activity: no activity above pH 7.0 Homo sapiens