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Literature summary for 3.6.1.7 extracted from
- Crystallization and properties of acylphosphatase from porcine skeletal muscle (1984), J. Biochem., 96, 313-320.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | activation | Sus scrofa |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| skeletal muscle enzyme | Sus scrofa |
General Stability
| General Stability | Organism |
|---|---|
| enzyme is very stable | Sus scrofa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 9000 | - |
skeletal muscle, 1 * 9000, SDS-PAGE | Sus scrofa |
| 11100 | - |
skeletal muscle, sedimentation equilibrium | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | less than 0.2 mol glucose equivalent per mol enzyme, no hexosamine | Sus scrofa |
| no modification | amount of sugar is insignificant | Sus scrofa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | skeletal muscle | Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7160 | - |
skeletal muscle, substrate benzoyl phosphate, 25°C | Sus scrofa |
| 7200 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, long-time stable | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Sus scrofa | carboxylate + phosphate | - |
? |  |
| benzoyl phosphate + H2O | - |
Sus scrofa | benzoate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | skeletal muscle, 1 * 9000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
assay at | Sus scrofa |
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