Literature summary for 3.6.1.68 extracted from

Liu, J.; Guan, Z.; Liu, H.; Qi, L.; Zhang, D.; Zou, T.; Yin, P.
Structural insights into the substrate recognition mechanism of Arabidopsis GPP-bound NUDX1 for noncanonical monoterpene biosynthesis (2018), Mol. Plant, 11, 218-221 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
E56A	inactive	Arabidopsis thaliana
F127N	the mutant shows reduced activity compared to the wild type enzyme	Arabidopsis thaliana
F127N/P129N	the mutant shows reduced activity compared to the wild type enzyme	Arabidopsis thaliana
F134N	the mutation decreases the enzyme activity by nearly half or more	Arabidopsis thaliana
F134N	the mutation leads to severely compromised hydrolase activity toward geranyl diphosphate	Arabidopsis thaliana
F78N	the mutant shows reduced activity compared to the wild type enzyme	Arabidopsis thaliana
F85N	the mutation decreases the enzyme activity by nearly half or more	Arabidopsis thaliana
H42A	the mutant shows strongly reduced activity compared to the wild type enzyme	Arabidopsis thaliana
H49A	the mutation exhibits little effect on the catalytic reaction	Arabidopsis thaliana
L38N	the mutant shows reduced activity compared to the wild type enzyme	Arabidopsis thaliana
L45N	the mutation leads to severely compromised hydrolase activity toward geranyl diphosphate	Arabidopsis thaliana
P129N	the mutant shows reduced activity compared to the wild type enzyme	Arabidopsis thaliana
P136N	the mutation exhibits little effect on the catalytic reaction	Arabidopsis thaliana
P136N	the mutation leads to severely compromised hydrolase activity toward geranyl diphosphate	Arabidopsis thaliana
R27A	the mutant shows strongly reduced activity compared to the wild type enzyme	Arabidopsis thaliana
R34A	the mutation leads to severely compromised hydrolase activity toward geranyl diphosphate	Arabidopsis thaliana
V10K	the mutation leads to the dissociation of the enzyme dimer	Arabidopsis thaliana
V13N	the mutant shows reduced activity compared to the wild type enzyme	Arabidopsis thaliana
V20N	the mutation decreases the enzyme activity by nearly half or more	Arabidopsis thaliana
Y87A	the mutant shows strongly reduced activity compared to the wild type enzyme	Arabidopsis thaliana
Y94A	the mutation leads to severely compromised hydrolase activity toward geranyl diphosphate	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranyl diphosphate + H2O	Arabidopsis thaliana	-	geranyl phosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate + H2O	-	Arabidopsis thaliana	geranyl phosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
Nudix1	-	Arabidopsis thaliana
NUDX1	-	Arabidopsis thaliana