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Literature summary for 3.6.1.67 extracted from
- A nudix enzyme removes pyrophosphate from dihydroneopterin triphosphate in the folate synthesis pathway of bacteria and plants (2004), J. Biol. Chem., 280, 5274-5280.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Lactococcus lactis |
| expression in Escherichia coli | Arabidopsis thaliana |
| expression in Escherichia coli with and without a C-terminal His tag | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0021 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.0, 30°C | Lactococcus lactis |  |
| 0.0021 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
| 0.147 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, no activity with Ca2+ | Arabidopsis thaliana | |
| Mg2+ | requires a physiological level (approximately 1 mM) of Mg2+ | Lactococcus lactis | |
| Mg2+ | the enzyme requires a physiological level of Mg2+, 70% of its maximal activity is reached at 1 mM Mg2+. No activation by 1 mM Ca2+ | Lactococcus lactis | |
| Mg2+ | the enzyme requires a physiological level of Mg2+, 70% of its maximal activity is reached at 1 mM Mg2+. No activation by 1 mM Ca2+ | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20400 | - |
1 * 20400, calculated from sequence | Lactococcus lactis |
| 20400 | - |
1 * 20400, calculated from sequence | Arabidopsis thaliana |
| 24200 | - |
gel filtration | Lactococcus lactis |
| 24200 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin 3'-triphosphate + H2O | Lactococcus lactis | the second step in the pterin branch of the folate synthesis pathway | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
| 7,8-dihydroneopterin 3'-triphosphate + H2O | Lactococcus lactis NZ9000 | the second step in the pterin branch of the folate synthesis pathway | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9CA40 | - |
- |
| Lactococcus lactis | D8KIT5 | - |
- |
| Lactococcus lactis NZ9000 | D8KIT5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0017 | - |
substrate: 7,8-dihydroneopterin 3'-triphosphate, pH 8.0, 30°C | Arabidopsis thaliana |
| 0.65 | - |
substrate: 7,8-dihydroneopterin 3'-triphosphate, pH 8.0, 30°C | Lactococcus lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydroneopterin 3'-triphosphate + H2O | the second step in the pterin branch of the folate synthesis pathway | Lactococcus lactis | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
| 7,8-dihydroneopterin 3'-triphosphate + H2O | no activity against deoxynucleoside triphosphates | Lactococcus lactis | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
| 7,8-dihydroneopterin 3'-triphosphate + H2O | the enzyme is far more active against dGTP, 8-oxo-GTP, dTTP, and other nucleoside and deoxynucleoside 3'-triphosphates than against 7,8-dihydroneopterin 3'-triphosphate | Arabidopsis thaliana | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
| 7,8-dihydroneopterin 3'-triphosphate + H2O | catalytic efficiency of the enzyme is far higher for dGTP | Arabidopsis thaliana | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
| 7,8-dihydroneopterin 3'-triphosphate + H2O | no detectable activity with GTP, ATP, TTP, CTP, the corresponding deoxynucleoside triphosphates, 8-oxo-dGTP, NADH, NAD, diadenosine tri- or tetraphosphate, ADP-ribose, UDP-glucose, or coenzyme A (the detection limit is less than 1% of the activity with 7,8-dihydroneopterin 3'-triphosphate) | Lactococcus lactis | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
| 7,8-dihydroneopterin 3'-triphosphate + H2O | the second step in the pterin branch of the folate synthesis pathway | Lactococcus lactis NZ9000 | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
| 7,8-dihydroneopterin 3'-triphosphate + H2O | no activity against deoxynucleoside triphosphates | Lactococcus lactis NZ9000 | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
| 7,8-dihydroneopterin 3'-triphosphate + H2O | no detectable activity with GTP, ATP, TTP, CTP, the corresponding deoxynucleoside triphosphates, 8-oxo-dGTP, NADH, NAD, diadenosine tri- or tetraphosphate, ADP-ribose, UDP-glucose, or coenzyme A (the detection limit is less than 1% of the activity with 7,8-dihydroneopterin 3'-triphosphate) | Lactococcus lactis NZ9000 | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 20400, calculated from sequence | Lactococcus lactis |
| monomer | 1 * 20400, calculated from sequence | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| At1g68760 | - |
Arabidopsis thaliana |
| At1g68760 protein | - |
Arabidopsis thaliana |
| AtNUDT1 | - |
Arabidopsis thaliana |
| DHNTP pyrophosphohydrolase | - |
Lactococcus lactis |
| DHNTP pyrophosphohydrolase | - |
Arabidopsis thaliana |
| dihydroneopterin triphosphate pyrophosphohydrolase | - |
Lactococcus lactis |
| dihydroneopterin triphosphate pyrophosphohydrolase | - |
Arabidopsis thaliana |
| folQ | - |
Lactococcus lactis |
| YlgG | - |
Lactococcus lactis |
| YlgG | former gene name | Lactococcus lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Lactococcus lactis |
| 30 | - |
assay at | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
| 0.21 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.0, 30°C | Lactococcus lactis | |
| 0.21 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Lactococcus lactis |
| 8 | - |
assay at | Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme functions in folate biosynthesis | Lactococcus lactis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0041 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
| 100 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.0, 30°C | Lactococcus lactis | |
| 100 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.0, 30°C | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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