BRENDA - Enzyme Database
show all sequences of 3.6.1.67

A nudix enzyme removes pyrophosphate from dihydroneopterin triphosphate in the folate synthesis pathway of bacteria and plants

Klaus, S.M.; Wegkamp, A.; Sybesma, W.; Hugenholtz, J.; Gregory, J.F. 3rd, Hanson, A.D.; J. Biol. Chem. 280, 5274-5280 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Lactococcus lactis
expression in Escherichia coli; expression in Escherichia coli with and without a C-terminal His tag
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0021
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
0.0021
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Lactococcus lactis
0.147
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, no activity with Ca2+; the enzyme requires a physiological level of Mg2+, 70% of its maximal activity is reached at 1 mM Mg2+. No activation by 1 mM Ca2+
Arabidopsis thaliana
Mg2+
requires a physiological level (approximately 1 mM) of Mg2+; the enzyme requires a physiological level of Mg2+, 70% of its maximal activity is reached at 1 mM Mg2+. No activation by 1 mM Ca2+
Lactococcus lactis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20400
-
1 * 20400, calculated from sequence
Arabidopsis thaliana
20400
-
1 * 20400, calculated from sequence
Lactococcus lactis
24200
-
gel filtration
Arabidopsis thaliana
24200
-
gel filtration
Lactococcus lactis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7,8-dihydroneopterin 3'-triphosphate + H2O
Lactococcus lactis
the second step in the pterin branch of the folate synthesis pathway
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
Lactococcus lactis NZ9000
the second step in the pterin branch of the folate synthesis pathway
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9CA40
-
-
Lactococcus lactis
D8KIT5
-
-
Lactococcus lactis NZ9000
D8KIT5
-
-
Purification (Commentary)
Commentary
Organism
-
Arabidopsis thaliana
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0017
-
substrate: 7,8-dihydroneopterin 3'-triphosphate, pH 8.0, 30C
Arabidopsis thaliana
0.65
-
substrate: 7,8-dihydroneopterin 3'-triphosphate, pH 8.0, 30C
Lactococcus lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7,8-dihydroneopterin 3'-triphosphate + H2O
the second step in the pterin branch of the folate synthesis pathway
727852
Lactococcus lactis
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
no activity against deoxynucleoside triphosphates
727852
Lactococcus lactis
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
the enzyme is far more active against dGTP, 8-oxo-GTP, dTTP, and other nucleoside and deoxynucleoside 3'-triphosphates than against 7,8-dihydroneopterin 3'-triphosphate
727852
Arabidopsis thaliana
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
catalytic efficiency of the enzyme is far higher for dGTP
727852
Arabidopsis thaliana
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
no detectable activity with GTP, ATP, TTP, CTP, the corresponding deoxynucleoside triphosphates, 8-oxo-dGTP, NADH, NAD, diadenosine tri- or tetraphosphate, ADP-ribose, UDP-glucose, or coenzyme A (the detection limit is less than 1% of the activity with 7,8-dihydroneopterin 3'-triphosphate)
727852
Lactococcus lactis
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
the second step in the pterin branch of the folate synthesis pathway
727852
Lactococcus lactis NZ9000
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
no activity against deoxynucleoside triphosphates
727852
Lactococcus lactis NZ9000
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
no detectable activity with GTP, ATP, TTP, CTP, the corresponding deoxynucleoside triphosphates, 8-oxo-dGTP, NADH, NAD, diadenosine tri- or tetraphosphate, ADP-ribose, UDP-glucose, or coenzyme A (the detection limit is less than 1% of the activity with 7,8-dihydroneopterin 3'-triphosphate)
727852
Lactococcus lactis NZ9000
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 20400, calculated from sequence
Arabidopsis thaliana
monomer
1 * 20400, calculated from sequence
Lactococcus lactis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Arabidopsis thaliana
30
-
assay at
Lactococcus lactis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0006
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
0.21
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
0.21
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Lactococcus lactis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Arabidopsis thaliana
8
-
assay at
Lactococcus lactis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Lactococcus lactis
expression in Escherichia coli; expression in Escherichia coli with and without a C-terminal His tag
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0021
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
0.0021
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Lactococcus lactis
0.147
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, no activity with Ca2+; the enzyme requires a physiological level of Mg2+, 70% of its maximal activity is reached at 1 mM Mg2+. No activation by 1 mM Ca2+
Arabidopsis thaliana
Mg2+
requires a physiological level (approximately 1 mM) of Mg2+; the enzyme requires a physiological level of Mg2+, 70% of its maximal activity is reached at 1 mM Mg2+. No activation by 1 mM Ca2+
Lactococcus lactis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20400
-
1 * 20400, calculated from sequence
Arabidopsis thaliana
20400
-
1 * 20400, calculated from sequence
Lactococcus lactis
24200
-
gel filtration
Arabidopsis thaliana
24200
-
gel filtration
Lactococcus lactis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7,8-dihydroneopterin 3'-triphosphate + H2O
Lactococcus lactis
the second step in the pterin branch of the folate synthesis pathway
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
Lactococcus lactis NZ9000
the second step in the pterin branch of the folate synthesis pathway
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Arabidopsis thaliana
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0017
-
substrate: 7,8-dihydroneopterin 3'-triphosphate, pH 8.0, 30C
Arabidopsis thaliana
0.65
-
substrate: 7,8-dihydroneopterin 3'-triphosphate, pH 8.0, 30C
Lactococcus lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7,8-dihydroneopterin 3'-triphosphate + H2O
the second step in the pterin branch of the folate synthesis pathway
727852
Lactococcus lactis
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
no activity against deoxynucleoside triphosphates
727852
Lactococcus lactis
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
the enzyme is far more active against dGTP, 8-oxo-GTP, dTTP, and other nucleoside and deoxynucleoside 3'-triphosphates than against 7,8-dihydroneopterin 3'-triphosphate
727852
Arabidopsis thaliana
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
catalytic efficiency of the enzyme is far higher for dGTP
727852
Arabidopsis thaliana
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
no detectable activity with GTP, ATP, TTP, CTP, the corresponding deoxynucleoside triphosphates, 8-oxo-dGTP, NADH, NAD, diadenosine tri- or tetraphosphate, ADP-ribose, UDP-glucose, or coenzyme A (the detection limit is less than 1% of the activity with 7,8-dihydroneopterin 3'-triphosphate)
727852
Lactococcus lactis
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
the second step in the pterin branch of the folate synthesis pathway
727852
Lactococcus lactis NZ9000
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
no activity against deoxynucleoside triphosphates
727852
Lactococcus lactis NZ9000
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
7,8-dihydroneopterin 3'-triphosphate + H2O
no detectable activity with GTP, ATP, TTP, CTP, the corresponding deoxynucleoside triphosphates, 8-oxo-dGTP, NADH, NAD, diadenosine tri- or tetraphosphate, ADP-ribose, UDP-glucose, or coenzyme A (the detection limit is less than 1% of the activity with 7,8-dihydroneopterin 3'-triphosphate)
727852
Lactococcus lactis NZ9000
7,8-dihydroneopterin 3'-phosphate + diphosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 20400, calculated from sequence
Arabidopsis thaliana
monomer
1 * 20400, calculated from sequence
Lactococcus lactis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Arabidopsis thaliana
30
-
assay at
Lactococcus lactis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0006
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
0.21
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
0.21
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Lactococcus lactis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Arabidopsis thaliana
8
-
assay at
Lactococcus lactis
General Information
General Information
Commentary
Organism
physiological function
the enzyme functions in folate biosynthesis
Lactococcus lactis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme functions in folate biosynthesis
Lactococcus lactis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0041
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
100
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
100
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Lactococcus lactis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0041
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
100
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Arabidopsis thaliana
100
-
7,8-dihydroneopterin 3'-triphosphate
pH 8.0, 30C
Lactococcus lactis
Other publictions for EC 3.6.1.67
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728808
Gabelli
Structure and function of the ...
Escherichia coli
Structure
15
1014-1022
2007
-
-
-
1
-
-
-
2
-
-
-
2
-
3
-
-
-
-
-
-
1
-
4
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
1
-
4
-
1
-
-
2
1
-
-
-
-
3
3
-
2
2
727354
Hori
Dual hydrolysis of diphosphate ...
Escherichia coli
DNA Repair
4
33-39
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727852
Klaus
A nudix enzyme removes pyropho ...
Arabidopsis thaliana, Lactococcus lactis, Lactococcus lactis NZ9000
J. Biol. Chem.
280
5274-5280
2004
-
-
2
-
-
-
-
3
-
2
4
2
-
7
-
-
1
-
-
-
2
-
8
2
2
-
-
3
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
2
4
2
-
-
-
1
-
-
2
-
8
2
2
-
-
3
2
-
-
-
-
1
1
-
3
3
729956
OHandley
Escherichia coli orf17 codes f ...
Escherichia coli
J. Biol. Chem.
271
24649-24654
1996
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727800
Suzuki
The biosynthesis of folic acid ...
Escherichia coli
J. Biol. Chem.
249
2405-2410
1974
-
-
-
-
-
-
5
1
-
1
1
1
-
2
-
-
1
-
-
-
-
-
4
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
-
2
2
1
-
-
-
2
-
-
-
-
4
-
2
-
2
-
2
2
-
-
-
1
1
-
-
-