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Literature summary for 3.6.1.66 extracted from

  • Awwad, K.; Desai, A.; Smith, C.; Sommerhalter, M.
    Structural and functional characterization of a noncanonical nucleoside triphosphate pyrophosphatase from Thermotoga maritima (2013), Acta Crystallogr. Sect. D, 69, 184-193 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.66

Crystallization (Commentary)

Crystallization (Comment) Organism
protein X-ray structure of the enzyme with bound IMP is obtained at 2.15 A resolution and compare it with structures of unliganded TM0159 and other noncanonical NTPases Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 ITP pH 9.0, 50°C Thermotoga maritima
0.07
-
 dITP pH 9.0, 50°C Thermotoga maritima
0.11
-
 XTP pH 9.0, 50°C Thermotoga maritima
0.45
-
 dITP pH 9.0, 80°C Thermotoga maritima
0.51
-
 ITP pH 9.0, 80°C Thermotoga maritima
1.69
-
 XTP pH 9.0, 80°C Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement for Mg2+. At 10 mM Mg2+ concentration, the enzyme reaches a plateau of optimum activity. Other divalent and monovalent cations barely support the catalytic activity of TM0159. Samples containing up to 100 mM Mn2+, Zn2+, Co2+, Cu2+, Na+, K+ or Li+ show less than 10% of the activity measured at 10 mM Mg2+ Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WY06
-
-
Thermotoga maritima DSM 3109 Q9WY06
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dITP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima dTDP + phosphate
-
 ?
dITP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima DSM 3109 dTDP + phosphate
-
 ?
ITP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima IDP + phosphate
-
 ?
ITP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima DSM 3109 IDP + phosphate
-
 ?
XTP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima XDP + phosphate
-
 ?
XTP + H2O ITP and dITP are slightly preferred over XTP Thermotoga maritima DSM 3109 XDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer crystal structure shows a tetramer with two possible dimer interfaces Thermotoga maritima

Synonyms

Synonyms Comment Organism
TM0159
-
 Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22
-
 no significant activity is detected at room temperature Thermotoga maritima
50 80 assay at Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
 XTP pH 9.0, 50°C Thermotoga maritima
3.9
-
 ITP pH 9.0, 50°C Thermotoga maritima
5.46
-
 dITP pH 9.0, 50°C Thermotoga maritima
13.87
-
 ITP pH 9.0, 80°C Thermotoga maritima
18.68
-
 dITP pH 9.0, 80°C Thermotoga maritima
22.08
-
 XTP pH 9.0, 80°C Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
9
-
 preference for alkaline conditions Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13.1
-
 XTP pH 9.0, 80°C Thermotoga maritima
24.5
-
 XTP pH 9.0, 50°C Thermotoga maritima
27.2
-
 ITP pH 9.0, 80°C Thermotoga maritima
41.5
-
 dITP pH 9.0, 80°C Thermotoga maritima
65
-
 ITP pH 9.0, 50°C Thermotoga maritima
78
-
 dITP pH 9.0, 50°C Thermotoga maritima