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Literature summary for 3.6.1.65 extracted from

  • Rihtman, B.; Bowman-Grahl, S.; Millard, A.; Corrigan, R.M.; Clokie, M.R.J.; Scanlan, D.J.
    Cyanophage MazG is a pyrophosphohydrolase but unable to hydrolyse magic spot nucleotides (2019), Environ. Microbiol. Rep., 11, 448-455 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.65

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Synechococcus phage S-PM2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 dGTP pH and temperature not specified in the publication Synechococcus phage S-PM2
0.6
-
 UTP pH and temperature not specified in the publication Synechococcus phage S-PM2
0.85
-
 CTP pH and temperature not specified in the publication Synechococcus phage S-PM2
1.16
-
 dCTP pH and temperature not specified in the publication Synechococcus phage S-PM2
1.2
-
 dATP pH and temperature not specified in the publication Synechococcus phage S-PM2
1.23
-
 dTTP pH and temperature not specified in the publication Synechococcus phage S-PM2
1.43
-
 ATP pH and temperature not specified in the publication Synechococcus phage S-PM2
3.24
-
 dUTP pH and temperature not specified in the publication Synechococcus phage S-PM2
4.86
-
 2-hydroxy-dATP pH and temperature not specified in the publication Synechococcus phage S-PM2

Organism

Organism UniProt Comment Textmining
Synechococcus phage S-PM2
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-dATP + H2O
-
 Synechococcus phage S-PM2 2-hydroxy-dAMP + diphosphate
-
 ?
ATP + H2O
-
 Synechococcus phage S-PM2 AMP + diphosphate
-
 ?
CTP + H2O
-
 Synechococcus phage S-PM2 CMP + diphosphate
-
 ?
dATP + H2O
-
 Synechococcus phage S-PM2 dAMP + diphosphate
-
 ?
dCTP + H2O preferred substrate Synechococcus phage S-PM2 dCMP + diphosphate
-
 ?
dGTP + H2O preferred substrate Synechococcus phage S-PM2 dGMP + diphosphate
-
 ?
dTTP + H2O
-
 Synechococcus phage S-PM2 dTMP + diphosphate
-
 ?
dUTP + H2O
-
 Synechococcus phage S-PM2 dUMP + diphosphate
-
 ?
GTP + H2O
-
 Synechococcus phage S-PM2 GMP + diphosphate
-
 ?
additional information the enzyme shows no binding or hydrolysis activity against (p)ppGpp and 8-oxo-dGTP Synechococcus phage S-PM2 ?
-
-
UTP + H2O
-
 Synechococcus phage S-PM2 UMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
MazG
-
 Synechococcus phage S-PM2

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
 GTP pH and temperature not specified in the publication Synechococcus phage S-PM2
1.05
-
 dATP pH and temperature not specified in the publication Synechococcus phage S-PM2
1.48
-
 ATP pH and temperature not specified in the publication Synechococcus phage S-PM2
1.62
-
 CTP pH and temperature not specified in the publication Synechococcus phage S-PM2
1.93
-
 2-hydroxy-dATP pH and temperature not specified in the publication Synechococcus phage S-PM2
2.15
-
 UTP pH and temperature not specified in the publication Synechococcus phage S-PM2
3.68
-
 dTTP pH and temperature not specified in the publication Synechococcus phage S-PM2
4.94
-
 dUTP pH and temperature not specified in the publication Synechococcus phage S-PM2
5.75
-
 dCTP pH and temperature not specified in the publication Synechococcus phage S-PM2
6.67
-
 dGTP pH and temperature not specified in the publication Synechococcus phage S-PM2