Cloned (Comment) | Organism |
---|---|
expression as glutathione S-transferase fusion protein | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0557 | - |
5-hydroxy-CTP | pH 8.5, 30°C | Escherichia coli | |
0.428 | - |
CTP | pH 8.5, 30°C | Escherichia coli | |
1.72 | - |
dCTP | pH 8.5, 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, activity constant 1.2 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77788 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxy-CTP + H2O | - |
Escherichia coli | 5-hydroxy-CMP + diphosphate | - |
? | |
5-hydroxy-dCTP + H2O | - |
Escherichia coli | 5-hydroxy-dCMP + diphosphate | 4.3% of the activity with 5-hydroxy-CTP | ? | |
CTP + H2O | - |
Escherichia coli | CMP + diphosphate | - |
? | |
dCTP + H2O | - |
Escherichia coli | dCMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nudG | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.28 | - |
CTP | pH 8.5, 30°C | Escherichia coli | |
17.4 | - |
dCTP | pH 8.5, 30°C | Escherichia coli | |
35.1 | - |
5-hydroxy-CTP | pH 8.5, 30°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.1 | - |
dCTP | pH 8.5, 30°C | Escherichia coli | |
21.7 | - |
CTP | pH 8.5, 30°C | Escherichia coli | |
630 | - |
5-hydroxy-CTP | pH 8.5, 30°C | Escherichia coli |