Cloned (Comment) | Organism |
---|---|
cloned into the pSPORT1 vector and expressed in Escherichia coli | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
phnM | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | in vivo complementation of the strains, in which phnC to phnP or phnH to phnP are deleted, with plasmids carrying various fragments of the phn operon reveal that the expression of phnC-phnP gene products is essential to restore growth on minimal medium with phosphonate as the sole phosphorus source, while phnG-phnM gene products are required for carbon-phosphorus lyase activity. The minimum size of the DNA required for the whole-cell carbon-phosphorus lyase activity is determined to be a 5.8-kb fragment, encompassing the phnG to phnM genes | Escherichia coli |