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Literature summary for 3.6.1.61 extracted from

  • Bessman, M.J.; Walsh, J.D.; Dunn, C.A.; Swaminathan, J.; Weldon, J.E.; Shen, J.
    The gene ygdP, associated with the invasiveness of Escherichia coli K1, designates a Nudix hydrolase, Orf176, active on adenosine (5')-pentaphospho-(5')-adenosine (Ap5A) (2001), J. Biol. Chem., 276, 37834-37838.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 P1,P4-bis(5'-adenosyl)tetraphosphate pH 9.0, 37°C Escherichia coli
0.36
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 9.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, Mg2+, Zn2+ or Mn2+. Maximal activity with 10 mM Mg2+ and to a lesser extent with Zn2+ and Mn2+ Escherichia coli
Mn2+ divalent cation required, Mg2+, Zn2+ or Mn2+. Maximal activity with 10 mM Mg2+ and to a lesser extent with Zn2+ and Mn2+ Escherichia coli
Zn2+ divalent cation required, Mg2+, Zn2+ or Mn2+. Maximal activity with 10 mM Mg2+ and to a lesser extent with Zn2+ and Mn2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A776
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information YgdP does not act on other nucleoside diphosphate derivatives such as ADP-ribose, NADH, UDP-glucose and P1,P3-bis(5'-adenosyl)triphosphate Escherichia coli ?
-
 ?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
-
 Escherichia coli ATP + AMP
-
 ?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O preferred substrate Escherichia coli ATP + ADP
-
 ?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O hydrolyzed at about 70% compared to P1,P5-bis(5'-adenosyl)pentaphosphate Escherichia coli 2 ATP
-
 ?

Synonyms

Synonyms Comment Organism
ygdP
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 P1,P4-bis(5'-adenosyl)tetraphosphate pH 9.0, 37°C Escherichia coli
1
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 9.0, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
 Escherichia coli
9
-
 assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.43
-
 P1,P4-bis(5'-adenosyl)tetraphosphate pH 9.0, 37°C Escherichia coli
2.78
-
 P1,P5-bis(5'-adenosyl)pentaphosphate pH 9.0, 37°C Escherichia coli