BRENDA - Enzyme Database
show all sequences of 3.6.1.61

The gene ygdP, associated with the invasiveness of Escherichia coli K1, designates a Nudix hydrolase, Orf176, active on adenosine (5')-pentaphospho-(5')-adenosine (Ap5A)

Bessman, M.J.; Walsh, J.D.; Dunn, C.A.; Swaminathan, J.; Weldon, J.E.; Shen, J.; J. Biol. Chem. 276, 37834-37838 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
P1,P4-bis(5'-adenosyl)tetraphosphate
pH 9.0, 37C
Escherichia coli
0.36
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 9.0, 37C
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
divalent cation required, Mg2+, Zn2+ or Mn2+. Maximal activity with 10 mM Mg2+ and to a lesser extent with Zn2+ and Mn2+
Escherichia coli
Mn2+
divalent cation required, Mg2+, Zn2+ or Mn2+. Maximal activity with 10 mM Mg2+ and to a lesser extent with Zn2+ and Mn2+
Escherichia coli
Zn2+
divalent cation required, Mg2+, Zn2+ or Mn2+. Maximal activity with 10 mM Mg2+ and to a lesser extent with Zn2+ and Mn2+
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P0A776
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
YgdP does not act on other nucleoside diphosphate derivatives such as ADP-ribose, NADH, UDP-glucose and P1,P3-bis(5'-adenosyl)triphosphate
715453
Escherichia coli
?
-
-
-
-
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
-
715453
Escherichia coli
ATP + AMP
-
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
preferred substrate
715453
Escherichia coli
ATP + ADP
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
hydrolyzed at about 70% compared to P1,P5-bis(5'-adenosyl)pentaphosphate
715453
Escherichia coli
2 ATP
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.1
-
P1,P4-bis(5'-adenosyl)tetraphosphate
pH 9.0, 37C
Escherichia coli
1
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 9.0, 37C
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Escherichia coli
9
-
assay at
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
P1,P4-bis(5'-adenosyl)tetraphosphate
pH 9.0, 37C
Escherichia coli
0.36
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 9.0, 37C
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
divalent cation required, Mg2+, Zn2+ or Mn2+. Maximal activity with 10 mM Mg2+ and to a lesser extent with Zn2+ and Mn2+
Escherichia coli
Mn2+
divalent cation required, Mg2+, Zn2+ or Mn2+. Maximal activity with 10 mM Mg2+ and to a lesser extent with Zn2+ and Mn2+
Escherichia coli
Zn2+
divalent cation required, Mg2+, Zn2+ or Mn2+. Maximal activity with 10 mM Mg2+ and to a lesser extent with Zn2+ and Mn2+
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
YgdP does not act on other nucleoside diphosphate derivatives such as ADP-ribose, NADH, UDP-glucose and P1,P3-bis(5'-adenosyl)triphosphate
715453
Escherichia coli
?
-
-
-
-
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
-
715453
Escherichia coli
ATP + AMP
-
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
preferred substrate
715453
Escherichia coli
ATP + ADP
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
hydrolyzed at about 70% compared to P1,P5-bis(5'-adenosyl)pentaphosphate
715453
Escherichia coli
2 ATP
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.1
-
P1,P4-bis(5'-adenosyl)tetraphosphate
pH 9.0, 37C
Escherichia coli
1
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 9.0, 37C
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Escherichia coli
9
-
assay at
Escherichia coli
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.43
-
P1,P4-bis(5'-adenosyl)tetraphosphate
pH 9.0, 37C
Escherichia coli
2.78
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 9.0, 37C
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.43
-
P1,P4-bis(5'-adenosyl)tetraphosphate
pH 9.0, 37C
Escherichia coli
2.78
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 9.0, 37C
Escherichia coli
Other publictions for EC 3.6.1.61
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714017
Zheng
Homology modeling and substrat ...
Thermus thermophilus
Biochem. Biophys. Res. Commun.
333
881-887
2005
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715466
Iwai
The Nudix hydrolase Ndx1 from ...
Thermus thermophilus
J. Biol. Chem.
279
21732-21739
2004
-
-
1
-
4
-
3
1
-
5
1
1
-
1
-
-
1
3
-
-
-
-
4
1
1
-
1
1
1
-
1
-
3
1
1
-
-
1
-
-
4
-
1
3
3
1
-
5
1
1
-
-
-
1
-
-
-
-
4
1
1
-
1
1
1
-
1
1
-
-
-
-
1
1
715453
Bessman
The gene ygdP, associated with ...
Escherichia coli
J. Biol. Chem.
276
37834-37838
2001
-
-
1
-
-
-
-
2
-
3
1
-
-
1
-
-
1
-
-
-
-
-
4
-
1
-
-
2
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
3
1
-
-
-
-
1
-
-
-
-
4
-
1
-
-
2
2
-
-
-
-
-
-
-
2
2