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Literature summary for 3.6.1.60 extracted from

  • Olejnik, K.; Murcha, M.W.; Whelan, J.; Kraszewska, E.
    Cloning and characterization of AtNUDT13, a novel mitochondrial Arabidopsis thaliana Nudix hydrolase specific for long-chain diadenosine polyphosphates (2007), FEBS J., 274, 4877-4885.
    View publication on PubMed
Search for more literature for 3.6.1.60

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol the enzyme requires reducing conditions for optimal activity. The omission of dithiothreitol from the enzymatic reactions reduces its activity by 70% Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
fluoride
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.61
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Arabidopsis thaliana 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal activity with 5 mM Mg2+. No activity with Mn2+ Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O Arabidopsis thaliana
-
 adenosine 5'-tetraphosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q52K88
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP (2) Arabidopsis thaliana
a
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-tetraphosphate + ADP (1) Arabidopsis thaliana
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information diadenosine tetraphosphate and diadenosine triphosphate, adenosine nucleotides, diphosphoinositol polyphosphate and phosphoribosyl pyrophosphate are not hydrolyzed Arabidopsis thaliana ?
-
 ?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O P1,P5-bis(5'-adenosyl)pentaphosphate is hydrolyzed at 40% of the activity compared to P1,P6-bis(5'-adenosyl)hexaphosphate Arabidopsis thaliana adenosine 5'-tetraphosphate + AMP
-
 ?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
-
 Arabidopsis thaliana adenosine 5'-tetraphosphate + ADP
-
 ?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O preferred substrate. Hydrolysis of P1,P6-bis(5'-adenosyl)hexaphosphate with the formation of ADP or AMP Arabidopsis thaliana adenosine 5'-tetraphosphate + ADP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At3g26690
-
 Arabidopsis thaliana
AtNUDT13
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.8
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Arabidopsis thaliana
8.5
-
-
 Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.07
-
 pH 7.6, 37°C Arabidopsis thaliana fluoride

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.6, 37°C Arabidopsis thaliana