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Literature summary for 3.6.1.60 extracted from
- Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases (2002), BMC Biochem., 3, 20.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S39N | a polymorphism of hAps1 leads to the point mutation S39N | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fluoride | non-competitive inhibition; non-competitive inhibition | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hApsI-39N | Homo sapiens | |
| 0.013 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hApsI | Homo sapiens | |
| 0.019 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C, hAps2 | Homo sapiens | |
| 0.037 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hApsI | Homo sapiens | |
| 0.05 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hAps2 | Homo sapiens | |
| 0.05 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hApsI-39N | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity | Homo sapiens | |
| Cu2+ | a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity | Homo sapiens | |
| Mn2+ | a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity | Homo sapiens | |
| additional information | Mg2+, Ni2+ and Ca1+ are not able to activate the enzyme | Homo sapiens | |
| Zn2+ | a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18500 | - |
x * 18500, calculation from sequence | Homo sapiens |
| 18559 | - |
x * 18559, calculation from sequence | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| P1,P5-bis(5'-adenosyl)pentaphosphate + H2O | Homo sapiens | marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels | adenosine 5'-tetraphosphate + AMP | - |
? | |
| P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | Homo sapiens | marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels | adenosine 5'-pentaphosphate + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q8NFP7 | - |
- |
| Homo sapiens | Q96G61 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP | (2) | Homo sapiens | |
| P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-pentaphosphate + AMP | (1) | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| brain | high activity | Homo sapiens | - |
| GI-102 cell | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| heart | low activity | Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| lung | low activity | Homo sapiens | - |
| additional information | no signal for hAps1 is detected in placenta, liver, skeletal muscle, kidney, spleen, colon or peripheral blood leukocytes. No activity in breast GI-101 cells, lung LX-1 cells, colon CX-1 cells, lung GI-117 cells, colon GI-112 cells and pancreas GI-103 cells | Homo sapiens | - |
| additional information | no signal for hAps2 is found in skeletal muscle, thymus, small intestine, colon or peripheral blood leukocytes. No activity in tumour cell lines from breast GI-101, lung LX-1, colon CX-1, lung GI-117, colon GI-112 and pancreas GI-103 | Homo sapiens | - |
| ovary | - |
Homo sapiens | - |
| ovary | low activity | Homo sapiens | - |
| pancreas | - |
Homo sapiens | - |
| pancreas | high activity | Homo sapiens | - |
| PC-3 cell | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| prostate | - |
Homo sapiens | - |
| prostate | low activity | Homo sapiens | - |
| small intestine | low activity | Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
| testis | - |
Homo sapiens | - |
| testis | high activity | Homo sapiens | - |
| thymus | low activity | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| P1,P4-bis(5'-adenosyl)tetraphosphate + H2O | weak activity | Homo sapiens | ATP + AMP | - |
? | |
| P1,P5-bis(5'-adenosyl)pentaphosphate + H2O | marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels | Homo sapiens | adenosine 5'-tetraphosphate + AMP | - |
? | |
| P1,P5-bis(5'-adenosyl)pentaphosphate + H2O | marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity | Homo sapiens | adenosine 5'-tetraphosphate + AMP | - |
? | |
| P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels | Homo sapiens | adenosine 5'-pentaphosphate + AMP | - |
? | |
| P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity | Homo sapiens | adenosine 5'-pentaphosphate + AMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 18500, calculation from sequence | Homo sapiens |
| ? | x * 18559, calculation from sequence | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hAps1 | - |
Homo sapiens |
| hAps2 | - |
Homo sapiens |
| NUDT10 | - |
Homo sapiens |
| NUDT11 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C, hApsI-39N | Homo sapiens | |
| 0.17 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C, hApsI | Homo sapiens | |
| 0.2 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C, hAps2 | Homo sapiens | |
| 0.4 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hApsI | Homo sapiens | |
| 0.4 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hApsI-39N | Homo sapiens | |
| 0.8 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hAps2 | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Homo sapiens |
| 8.5 | 9 | - |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | pH 7.5: about 55% of maximal activity, pH 8.5-9: maximal activity | Homo sapiens |
| 7.5 | 9 | pH 7.5: about 60% of maximal activity, pH 9: about 90% of maximal activity | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
fluoride | pH 7.6, 37°C | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hApsI-39N | Homo sapiens | |
| 10.5 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C, hAps2 | Homo sapiens | |
| 10.8 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hApsI | Homo sapiens | |
| 13.1 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C, hApsI | Homo sapiens | |
| 14.5 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C, hApsI-39N | Homo sapiens | |
| 16 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C, hAps2 | Homo sapiens | |
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