BRENDA - Enzyme Database
show all sequences of 3.6.1.60

Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases

Leslie, N.R.; McLennan, A.G.; Safrany, S.T.; BMC Biochem. 3, 20 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
;
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
S39N
a polymorphism of hAps1 leads to the point mutation S39N
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
fluoride
non-competitive inhibition; non-competitive inhibition
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
0.013
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
0.019
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
0.037
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
0.05
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2; pH 7.6, 37°C, hApsI-39N
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
;
Homo sapiens
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity; a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
Cu2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity; a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
Mn2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity; a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
additional information
Mg2+, Ni2+ and Ca1+ are not able to activate the enzyme; Mg2+, Ni2+ and Ca1+ are not able to activate the enzyme
Homo sapiens
Zn2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity; a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18500
-
x * 18500, calculation from sequence
Homo sapiens
18559
-
x * 18559, calculation from sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
Homo sapiens
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
adenosine 5'-tetraphosphate + AMP
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
Homo sapiens
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
adenosine 5'-pentaphosphate + AMP
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q8NFP7
-
-
Homo sapiens
Q96G61
-
-
Purification (Commentary)
Commentary
Organism
;
Homo sapiens
Reaction
Reaction
Commentary
Organism
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP
(2)
Homo sapiens
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-pentaphosphate + AMP
(1)
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
; high activity
Homo sapiens
-
GI-102 cell
-
Homo sapiens
-
heart
; low activity
Homo sapiens
-
kidney
-
Homo sapiens
-
liver
-
Homo sapiens
-
lung
; low activity
Homo sapiens
-
additional information
no signal for hAps1 is detected in placenta, liver, skeletal muscle, kidney, spleen, colon or peripheral blood leukocytes. No activity in breast GI-101 cells, lung LX-1 cells, colon CX-1 cells, lung GI-117 cells, colon GI-112 cells and pancreas GI-103 cells; no signal for hAps2 is found in skeletal muscle, thymus, small intestine, colon or peripheral blood leukocytes. No activity in tumour cell lines from breast GI-101, lung LX-1, colon CX-1, lung GI-117, colon GI-112 and pancreas GI-103
Homo sapiens
-
ovary
; low activity
Homo sapiens
-
pancreas
; high activity
Homo sapiens
-
PC-3 cell
;
Homo sapiens
-
placenta
-
Homo sapiens
-
prostate
; low activity
Homo sapiens
-
small intestine
low activity
Homo sapiens
-
spleen
-
Homo sapiens
-
testis
; high activity
Homo sapiens
-
thymus
low activity
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
weak activity
714610
Homo sapiens
ATP + AMP
-
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
714610
Homo sapiens
adenosine 5'-tetraphosphate + AMP
-
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity
714610
Homo sapiens
adenosine 5'-tetraphosphate + AMP
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
714610
Homo sapiens
adenosine 5'-pentaphosphate + AMP
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity
714610
Homo sapiens
adenosine 5'-pentaphosphate + AMP
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 18500, calculation from sequence; x * 18559, calculation from sequence
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.16
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
0.17
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
0.2
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
0.4
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI; pH 7.6, 37°C, hApsI-39N
Homo sapiens
0.8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Homo sapiens
8.5
-
-
Homo sapiens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
pH 7.5: about 55% of maximal activity, pH 8.5-9: maximal activity; pH 7.5: about 60% of maximal activity, pH 9: about 90% of maximal activity
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.013
-
fluoride
pH 7.6, 37°C; pH 7.6, 37°C
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
S39N
a polymorphism of hAps1 leads to the point mutation S39N
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
fluoride
non-competitive inhibition
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.013
-
fluoride
pH 7.6, 37°C
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
0.013
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
0.019
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
0.037
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
0.05
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
0.05
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Homo sapiens
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
Cu2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
Mn2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
additional information
Mg2+, Ni2+ and Ca1+ are not able to activate the enzyme
Homo sapiens
Zn2+
a divalent cation is essential for activity, with Mn2+ by far the most effective between 2 and 6 mM. Cu2+ supports less than 30% and Zn2+ and Co2+ each less than 3% of the maximum activity
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18500
-
x * 18500, calculation from sequence
Homo sapiens
18559
-
x * 18559, calculation from sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
Homo sapiens
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
adenosine 5'-tetraphosphate + AMP
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
Homo sapiens
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
adenosine 5'-pentaphosphate + AMP
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
high activity
Homo sapiens
-
brain
-
Homo sapiens
-
GI-102 cell
-
Homo sapiens
-
heart
low activity
Homo sapiens
-
heart
-
Homo sapiens
-
kidney
-
Homo sapiens
-
liver
-
Homo sapiens
-
lung
low activity
Homo sapiens
-
lung
-
Homo sapiens
-
additional information
no signal for hAps1 is detected in placenta, liver, skeletal muscle, kidney, spleen, colon or peripheral blood leukocytes. No activity in breast GI-101 cells, lung LX-1 cells, colon CX-1 cells, lung GI-117 cells, colon GI-112 cells and pancreas GI-103 cells
Homo sapiens
-
additional information
no signal for hAps2 is found in skeletal muscle, thymus, small intestine, colon or peripheral blood leukocytes. No activity in tumour cell lines from breast GI-101, lung LX-1, colon CX-1, lung GI-117, colon GI-112 and pancreas GI-103
Homo sapiens
-
ovary
low activity
Homo sapiens
-
ovary
-
Homo sapiens
-
pancreas
high activity
Homo sapiens
-
pancreas
-
Homo sapiens
-
PC-3 cell
-
Homo sapiens
-
placenta
-
Homo sapiens
-
prostate
low activity
Homo sapiens
-
prostate
-
Homo sapiens
-
small intestine
low activity
Homo sapiens
-
spleen
-
Homo sapiens
-
testis
high activity
Homo sapiens
-
testis
-
Homo sapiens
-
thymus
low activity
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
weak activity
714610
Homo sapiens
ATP + AMP
-
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
714610
Homo sapiens
adenosine 5'-tetraphosphate + AMP
-
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity
714610
Homo sapiens
adenosine 5'-tetraphosphate + AMP
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
marked preference for adenine over guanine nucleotides. The enzyme controls intracellular P1,P5-bis(5'-adenosyl)pentaphosphate and P1,P6-bis(5'-adenosyl)hexaphosphate levels
714610
Homo sapiens
adenosine 5'-pentaphosphate + AMP
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
marked preference for adenine over guanine nucleotides. The most rapidly metabolised substrate appears to be P1,P5-bis(5'-adenosyl)pentaphosphate, although P1,P6-bis(5'-adenosyl)hexaphosphate is bound with higher affinity
714610
Homo sapiens
adenosine 5'-pentaphosphate + AMP
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 18559, calculation from sequence
Homo sapiens
?
x * 18500, calculation from sequence
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.16
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
0.17
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
0.2
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
0.4
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI; pH 7.6, 37°C, hApsI-39N
Homo sapiens
0.8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Homo sapiens
8.5
-
-
Homo sapiens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
pH 7.5: about 55% of maximal activity, pH 8.5-9: maximal activity
Homo sapiens
7.5
9
pH 7.5: about 60% of maximal activity, pH 9: about 90% of maximal activity
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
10.5
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
10.8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
13.1
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
14.5
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
16
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
10.5
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
10.8
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
13.1
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI
Homo sapiens
14.5
-
P1,P6-bis(5'-adenosyl)hexaphosphate
pH 7.6, 37°C, hApsI-39N
Homo sapiens
16
-
P1,P5-bis(5'-adenosyl)pentaphosphate
pH 7.6, 37°C, hAps2
Homo sapiens
Other publictions for EC 3.6.1.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735159
Grisanzio
Genetic and functional analyse ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
109
11252-11257
2012
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714610
Leslie
Cloning and characterisation o ...
Homo sapiens
BMC Biochem.
3
20
2002
-
-
1
-
1
-
1
5
1
5
2
4
-
3
-
-
1
2
-
16
-
-
10
1
-
-
-
5
2
1
-
-
1
-
-
-
-
2
-
-
1
-
-
2
2
6
2
10
2
4
-
-
-
2
-
25
-
-
10
2
-
-
-
5
2
2
-
-
-
-
-
-
6
6
393825
Safrany
The diadenosine hexaphosphate ...
Homo sapiens
J. Biol. Chem.
274
21735-21740
1999
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