BRENDA - Enzyme Database
show all sequences of 3.6.1.6

Coexpression of ecto-5'-nucleotidase/CD73 with specific NTPDases differentially regulates adenosine formation in the rat liver

Fausther, M.; Lecka, J.; Soliman, E.; Kauffenstein, G.; Pelletier, J.; Sheung, N.; Dranoff, J.; Sevigny, J.; Am. J. Physiol. Gastrointest. Liver Physiol. 302, 447-459 (2012)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
plasma membrane
;
Rattus norvegicus
5886
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
required; required
Rattus norvegicus
Mg2+
required; required
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ADP + H2O
Rattus norvegicus
-
AMP + phosphate
-
-
?
ADP + H2O
Rattus norvegicus Sprague-Dawley
-
AMP + phosphate
-
-
?
ATP + 2 H2O
Rattus norvegicus
-
AMP + 2 phosphate
-
-
?
ATP + 2 H2O
Rattus norvegicus Sprague-Dawley
-
AMP + 2 phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
P97687
NTPDase1
-
Rattus norvegicus
Q5DRK1
NTPDase8
-
Rattus norvegicus Sprague-Dawley
P97687
NTPDase1
-
Rattus norvegicus Sprague-Dawley
Q5DRK1
NTPDase8
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
bile canaliculus
-
Rattus norvegicus
-
fibroblast
-
Rattus norvegicus
-
hepatocyte
restricted to the canalicular membrane domain of hepatocytes
Rattus norvegicus
-
liver
expression of isozymes NTPDase1, -2, and -8 in distinct liver compartments in normal and fibrotic rat liver; expression of isozymes NTPDase1, -2, and -8 in distinct liver compartments in normal and fibrotic rat liver
Rattus norvegicus
-
additional information
enzyme tissue localization by in situ immunohistochemistry, overview; enzyme tissue localization by in situ immunohistochemistry, overview
Rattus norvegicus
-
smooth muscle cell
-
Rattus norvegicus
-
vascular endothelial cell
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ADP + H2O
-
718543
Rattus norvegicus
AMP + phosphate
-
-
-
?
ADP + H2O
-
718543
Rattus norvegicus Sprague-Dawley
AMP + phosphate
-
-
-
?
ATP + 2 H2O
-
718543
Rattus norvegicus
AMP + 2 phosphate
-
-
-
?
ATP + 2 H2O
sequential dephosphorylation of ATP to ADP and then AMP
718543
Rattus norvegicus
AMP + 2 phosphate
-
-
-
?
ATP + 2 H2O
-
718543
Rattus norvegicus Sprague-Dawley
AMP + 2 phosphate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
ADPase
-
Rattus norvegicus
CD39
-
Rattus norvegicus
NTPDase1
-
Rattus norvegicus
NTPDase1/CD39
-
Rattus norvegicus
NTPDase8
-
Rattus norvegicus
nucleoside triphosphate diphosphohydrolase
-
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at; assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at; assay at
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
plasma membrane
-
Rattus norvegicus
5886
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Rattus norvegicus
Mg2+
required
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ADP + H2O
Rattus norvegicus
-
AMP + phosphate
-
-
?
ADP + H2O
Rattus norvegicus Sprague-Dawley
-
AMP + phosphate
-
-
?
ATP + 2 H2O
Rattus norvegicus
-
AMP + 2 phosphate
-
-
?
ATP + 2 H2O
Rattus norvegicus Sprague-Dawley
-
AMP + 2 phosphate
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
bile canaliculus
-
Rattus norvegicus
-
fibroblast
-
Rattus norvegicus
-
hepatocyte
restricted to the canalicular membrane domain of hepatocytes
Rattus norvegicus
-
liver
expression of isozymes NTPDase1, -2, and -8 in distinct liver compartments in normal and fibrotic rat liver
Rattus norvegicus
-
additional information
enzyme tissue localization by in situ immunohistochemistry, overview
Rattus norvegicus
-
smooth muscle cell
-
Rattus norvegicus
-
vascular endothelial cell
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ADP + H2O
-
718543
Rattus norvegicus
AMP + phosphate
-
-
-
?
ADP + H2O
-
718543
Rattus norvegicus Sprague-Dawley
AMP + phosphate
-
-
-
?
ATP + 2 H2O
-
718543
Rattus norvegicus
AMP + 2 phosphate
-
-
-
?
ATP + 2 H2O
sequential dephosphorylation of ATP to ADP and then AMP
718543
Rattus norvegicus
AMP + 2 phosphate
-
-
-
?
ATP + 2 H2O
-
718543
Rattus norvegicus Sprague-Dawley
AMP + 2 phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Rattus norvegicus
General Information
General Information
Commentary
Organism
metabolism
plasma membrane-bound NTPDases, namely NTPDase1/CD39, NTPDase2/CD39L1, and NTPDase8, represent the major liver ectonucleotidase activities; plasma membrane-bound NTPDases, namely NTPDase1/CD39, NTPDase2/CD39L1, and NTPDase8, represent the major liver ectonucleotidase activities
Rattus norvegicus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
plasma membrane-bound NTPDases, namely NTPDase1/CD39, NTPDase2/CD39L1, and NTPDase8, represent the major liver ectonucleotidase activities
Rattus norvegicus
Other publictions for EC 3.6.1.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751452
Paganini
Calcium activated nucleotidas ...
Mus musculus
Matrix Biol.
81
70-90
2018
-
-
-
-
1
-
-
-
2
1
-
1
-
2
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
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733220
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6
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-
-
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1
-
-
2
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733655
Krug
The ATP/ADP substrate specific ...
Toxoplasma gondii
ChemBioChem
14
2292-2300
2013
-
-
-
1
3
-
-
4
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
4
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
718543
Fausther
Coexpression of ecto-5'-nucleo ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Am. J. Physiol. Gastrointest. Liver Physiol.
302
447-459
2012
-
-
-
-
-
-
-
-
1
2
-
8
-
4
-
-
-
-
-
7
-
-
9
-
8
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
4
-
8
-
-
-
-
-
9
-
-
9
-
2
-
-
-
2
-
-
-
-
1
2
-
-
-
718541
Lavoie
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300
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140
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-
-
8
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-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
1
-
-
-
1
-
-
-
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2
2
-
-
-
720736
Tanaka
Enzymatic activity of the soyb ...
Glycine max
Plant Physiol.
155
1988-1998
2011
-
-
1
-
4
-
1
2
-
2
-
-
-
1
-
-
1
-
-
3
-
-
9
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
2
-
2
-
-
-
-
-
1
-
3
-
-
9
1
-
-
-
-
-
-
-
-
-
2
2
-
2
2
711898
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NTPDase1 governs P2X7-dependen ...
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40
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-
-
-
-
-
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-
-
-
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-
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-
1
-
-
-
-
-
1
-
-
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
719196
Kauffenstein
The ecto-nucleotidase NTPDase1 ...
Mus musculus 129 SVJ x C57 BL/6, Mus musculus
Br. J. Pharmacol.
159
576-585
2010
-
-
-
-
1
-
1
-
-
1
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8
-
3
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-
-
-
-
5
-
-
8
-
4
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
8
-
-
-
-
-
5
-
-
8
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
698165
Martin-Satue
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Mus musculus, Mus musculus C57BL/6, Rattus norvegicus
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131
615-628
2009
-
-
2
-
2
-
-
-
6
2
-
3
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141
-
-
-
-
-
28
-
-
6
-
16
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
6
2
-
3
-
-
-
-
-
28
-
-
6
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
669238
Uccelletti
ire-1-dependent transcriptiona ...
Caenorhabditis elegans
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27390-27398
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1
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1
-
-
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1
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-
-
-
-
-
-
-
2
-
3
-
-
-
-
2
-
-
-
-
-
-
1
-
1
-
-
-
-
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-
-
1
1
3
-
-
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-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
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-
-
670350
Nishiwaki
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-
-
-
-
-
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1
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1
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-
-
-
-
3
-
2
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-
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1
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1
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-
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-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654678
Ivanenkov
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Homo sapiens
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-
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1
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1
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1
1
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3
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1
1
-
1
1
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-
10
1
2
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
1
1
-
1
-
1
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2
1
-
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1
1
1
1
1
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-
10
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-
-
1
-
-
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1
1
2
2
1
1
-
6
-
-
-
-
-
1
-
-
8
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
2
2
1
1
-
-
-
-
-
1
-
-
8
1
-
-
-
-
1
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-
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Mikami
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-
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3
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1
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1
2
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3
1
1
-
-
1
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
2
3
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6
1
5
1
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-
-
-
1
-
1
2
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3
1
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1
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1
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1
-
-
-
-
-
2
3
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3
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-
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-
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1
-
-
1
1
-
8
1
-
-
-
-
-
3
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-
-
1
-
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1
-
-
-
-
-
-
-
2
1
3
-
3
1
1
-
-
-
1
-
1
1
-
8
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
210202
Sano
Type B nucleoside-diphosphatas ...
Rattus norvegicus
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171
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1988
-
-
-
-
-
-
10
4
1
8
1
-
-
1
-
-
1
-
-
1
1
-
5
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
4
1
8
1
-
-
-
-
1
-
1
1
-
5
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
210203
De Groot
Loss of latent activity of liv ...
Rattus norvegicus
Biochim. Biophys. Acta
815
91-96
1985
-
1
-
-
-
1
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
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-
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1
-
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-
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1
-
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210204
Sano
Thiamine pyrophosphatase and n ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
118
292-298
1984
1
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
2
-
-
6
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
2
-
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6
-
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3
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210205
Ohkubo
Comparative studies on nucleos ...
Rattus norvegicus
Int. J. Biochem.
14
1075-1081
1982
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5
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1
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2
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210207
Brandan
Orientation and role of nucleo ...
Rattus norvegicus
Biochemistry
21
4640-4645
1982
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210208
Ohkubo
Purification and characterizat ...
Rattus norvegicus
Eur. J. Biochem.
112
111-118
1980
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3
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1
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1
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210209
Kawakita
Immobilization of nucleoside d ...
Rattus norvegicus
Arch. Biochem. Biophys.
204
326-330
1980
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210210
Finch
Nucleotide metabolism by micro ...
Oryctolagus cuniculus
Biochem. J.
177
925-930
1979
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210211
Kawakita
Allosteric properties of nucle ...
Rattus norvegicus
Biochem. J.
17
3546-3551
1978
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3
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210213
Ishibashi
Affinity purification and some ...
Rattus norvegicus
Biochim. Biophys. Acta
525
265-274
1978
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1
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210216
Shirasaka
Studies on mitochondrial and m ...
Rattus norvegicus
J. Biochem.
76
99-106
1974
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6
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6
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210217
Pinsley
Nucleoside diphosphatase from ...
Sus scrofa
Arch. Biochem. Biophys.
158
331-345
1973
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