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Literature summary for 3.6.1.6 extracted from

  • Mikami, S.; Suganuma, R.; Hori, H.; Mitsui, T.
    Purification and characterization of Golgi membrane-bound nucleoside diphosphatase from suspension-cultured cells of sycamore (Acer pseudoplatanus L.) (2001), Plant Biotechnol., 18, 259-265.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ATP IC50 for isoenzyme 1 is 6.5 mM, IC50 for isoenzyme 2 is 4.0 mM Acer pseudoplatanus
EDTA
-
Acer pseudoplatanus
additional information no inhibition by 10 mM ascorbate, 80 mM molybdate, 20 mM KF or 10 mM vanadate Acer pseudoplatanus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
GDP isoenzyme 1 Acer pseudoplatanus
0.53
-
UDP isoenzyme 2 Acer pseudoplatanus
0.65
-
IDP isoenzyme 1 Acer pseudoplatanus
0.68
-
IDP isoenzyme 2 Acer pseudoplatanus
0.77
-
GDP isoenzyme 2 Acer pseudoplatanus
0.8
-
UDP isoenzyme 1 Acer pseudoplatanus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane bound to Acer pseudoplatanus 139
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Acer pseudoplatanus
Co2+ required Acer pseudoplatanus
Mg2+ required Acer pseudoplatanus
Mn2+ required Acer pseudoplatanus
Zn2+ 20% of the activity with Mg2+ Acer pseudoplatanus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel filtration Acer pseudoplatanus

Organism

Organism UniProt Comment Textmining
Acer pseudoplatanus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two isoforms Acer pseudoplatanus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Acer pseudoplatanus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
isoform 1 Acer pseudoplatanus
2.8
-
isoform 2 Acer pseudoplatanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + H2O 110% of the activity with IDP Acer pseudoplatanus GMP + phosphate
-
?
IDP + H2O
-
Acer pseudoplatanus IMP + phosphate
-
?
UDP + H2O 90% of the activity with UDP Acer pseudoplatanus UMP + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Acer pseudoplatanus

Synonyms

Synonyms Comment Organism
IDPase
-
Acer pseudoplatanus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
40 stable, isoenzyme 1 and 2 Acer pseudoplatanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Acer pseudoplatanus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4
-
IC50 for isoenzyme 2 is 4.0 mM Acer pseudoplatanus ATP
6.5
-
IC50 for isoenzyme 1 is 6.5 mM Acer pseudoplatanus ATP