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Literature summary for 3.6.1.6 extracted from

  • Failer, B.U.; Braun, N.; Zimmermann, H.
    Cloning, expression, and functional characterization of a Ca(2+)-dependent endoplasmic reticulum nucleoside diphosphatase (2002), J. Biol. Chem., 277, 36978-36986.
    View publication on PubMed
Search for more literature for 3.6.1.6

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.216
-
 UDP pH 7 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum associated with Rattus norvegicus 5783
-
pre-Golgi intermediate associated with Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on. AT 0.5 mM UDP, maximal activity is obtained at 1 mM Ca2+, higher Ca2+ concentrations reduce catalytic activity Rattus norvegicus
additional information insensitive to Mg2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45700
-
 x * 45700, calculation from nucleotide sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus the enzyme may support glycosylation reactions related to quality control in the endoplasmic reticulum ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q8K4Y7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP + H2O hydrolyzes nucleoside 5'-diphosphates in the order: UDP, GDP, IDP, GDP Rattus norvegicus CMP + phosphate
-
 ?
GDP + H2O hydrolyzes nucleoside 5'-diphosphates in the order: UDP, GDP, IDP, GDP Rattus norvegicus GMP + phosphate
-
 ?
GTP + H2O weak activity Rattus norvegicus GDP + phosphate
-
 ?
IDP + H2O hydrolyzes nucleoside 5'-diphosphates in the order: UDP, GDP, IDP, GDP Rattus norvegicus IMP + phosphate
-
 ?
additional information the enzyme may support glycosylation reactions related to quality control in the endoplasmic reticulum Rattus norvegicus ?
-
 ?
additional information no activity with ADP, nucleoside 5'-triphosphates are hydrolyzed to a minor extend, no hydrolysis of nucleoside 5'-monophosphates Rattus norvegicus ?
-
 ?
UDP + H2O hydrolyzes nucleoside 5'-diphosphates in the order: UDP, GDP, IDP, GDP Rattus norvegicus UMP + phosphate
-
 ?
UTP + H2O weak activity Rattus norvegicus UDP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 45700, calculation from nucleotide sequence Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 80% of maximal activity, pH 8.0: about 70% of maximal activity Rattus norvegicus