Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | enhances hydrolysis of GDP | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 0.5 mM, 50% inhibition | Rattus norvegicus | |
F- | 50 mM NaF, more than 80% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
GDP | - |
Rattus norvegicus | |
1.4 | - |
UDP | - |
Rattus norvegicus | |
1.7 | - |
IDP | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
Mg2+ | divalent cation required, maximum activity with Mg2+, half-maximal activity at 0.4 mM MgCl2 | Rattus norvegicus | |
Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | Rattus norvegicus | type L enzyme chiefly works in the degradation of nucleoside diphosphates | GMP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
type L isoenzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
348.4 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | no activity | Rattus norvegicus | AMP + phosphate | - |
? | |
CDP + H2O | - |
Rattus norvegicus | CMP + phosphate | - |
? | |
GDP + H2O | - |
Rattus norvegicus | GMP + phosphate | - |
? | |
GDP + H2O | type L enzyme chiefly works in the degradation of nucleoside diphosphates | Rattus norvegicus | GMP + phosphate | - |
? | |
IDP + H2O | - |
Rattus norvegicus | IMP + phosphate | - |
? | |
TDP + H2O | - |
Rattus norvegicus | TMP + phosphate | - |
? | |
TPP + H2O | - |
Rattus norvegicus | thiamine phosphate + phosphate | - |
? | |
UDP + H2O | - |
Rattus norvegicus | UMP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, L-type enzyme, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | hydrolysis of GDP | Rattus norvegicus |
7.5 | 8 | - |
Rattus norvegicus |
9 | - |
hydrolysis of TPP | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
diphosphate | - |
Rattus norvegicus |