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Literature summary for 3.6.1.6 extracted from

  • Yang, W.J.; Kim, K.W.; Lee, B.J.
    Rat brain nucleoside diphosphatase: purification and properties of type L isoenzyme (1994), Arch. Pharm. Res., 17, 256-262.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ATP enhances hydrolysis of GDP Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
diphosphate 0.5 mM, 50% inhibition Rattus norvegicus
F- 50 mM NaF, more than 80% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
GDP
-
Rattus norvegicus
1.4
-
UDP
-
Rattus norvegicus
1.7
-
IDP
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can partially replace Mg2+ in activation Rattus norvegicus
Mg2+ divalent cation required, maximum activity with Mg2+, half-maximal activity at 0.4 mM MgCl2 Rattus norvegicus
Mn2+ can partially replace Mg2+ in activation Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + H2O Rattus norvegicus type L enzyme chiefly works in the degradation of nucleoside diphosphates GMP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
type L isoenzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
348.4
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O no activity Rattus norvegicus AMP + phosphate
-
?
CDP + H2O
-
Rattus norvegicus CMP + phosphate
-
?
GDP + H2O
-
Rattus norvegicus GMP + phosphate
-
?
GDP + H2O type L enzyme chiefly works in the degradation of nucleoside diphosphates Rattus norvegicus GMP + phosphate
-
?
IDP + H2O
-
Rattus norvegicus IMP + phosphate
-
?
TDP + H2O
-
Rattus norvegicus TMP + phosphate
-
?
TPP + H2O
-
Rattus norvegicus thiamine phosphate + phosphate
-
?
UDP + H2O
-
Rattus norvegicus UMP + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, L-type enzyme, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9 hydrolysis of GDP Rattus norvegicus
7.5 8
-
Rattus norvegicus
9
-
hydrolysis of TPP Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
diphosphate
-
Rattus norvegicus