Literature summary for 3.6.1.57 extracted from

Liu, F.; Tanner, M.E.
PseG of pseudaminic acid biosynthesis: a UDP-sugar hydrolase as a masked glycosyltransferase (2006), J. Biol. Chem., 281, 20902-20909.

Search for more literature for 3.6.1.57

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Campylobacter jejuni

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	weak inhibition	Campylobacter jejuni

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.174	-	UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose	pH 7.5, 37°C	Campylobacter jejuni

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	this enzyme is independent of the presence of divalent metal ions	Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O	Campylobacter jejuni	the enzyme is involved in biosynthesis of pseudaminic acid (i.e. 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-nonulosonic acid). The large value of the specificity constant suggests that hydrolysis of UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose is the biologically relevant reaction catalyzed by this enzyme	2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + UDP	-	?

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	Q0P8U5	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Campylobacter jejuni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O	the enzyme is involved in biosynthesis of pseudaminic acid (i.e. 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-nonulosonic acid). The large value of the specificity constant suggests that hydrolysis of UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose is the biologically relevant reaction catalyzed by this enzyme	Campylobacter jejuni	2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + UDP	-	?
UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O	catalyzes the reaction via a C-O bond cleavage mechanism with inversion of stereochemistry at C-1. No detectable activity with UDP-GlcNAc and an extremely low level of activity (less than 1%) with the biosynthetic precursor, UDP-4-amino-2,4,6-trideoxy-beta-L-AltNAc, indicating that the enzyme exhibits substrate specificity. A solvent derived oxygen atom is incorporated into the sugar during hydrolysis and is consistent with a C-O bond cleavage mechanism in which water directly attacks the anomeric carbon to displace UDP	Campylobacter jejuni	2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + UDP	-	?

Synonyms

Synonyms	Comment	Organism
Cj1312	-	Campylobacter jejuni
PseG	-	Campylobacter jejuni
UDP-6-deoxy-AltdiNAc hydrolase	-	Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
26.6	-	UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose	pH 7.5, 37°C	Campylobacter jejuni

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Campylobacter jejuni

General Information

General Information	Comment	Organism
physiological function	the flagellin proteins in pathogenic bacteria such as Campylobacter jejuni and Helicobacter pylori are heavily glycosylated with the pseudaminic acid (i.e. 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-nonulosonic acid). The presence of this posttranslational modification is absolutely required for assembly of functional flagella. Since motility is required for colonization, pseudaminic acid biosynthesis represents a virulence factor in these bacteria	Campylobacter jejuni

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
152.9	-	UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose	pH 7.5, 37°C	Campylobacter jejuni

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Release 2023.1 (January 2023)