Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with 8-oxo-dGTPor 2-oxo-dATP, using 1.0 M sodium citrate, 0.1 M Tris-HCl, pH 7.0, and 0.2 M NaCl, or 1.0 M sodium citrate, 0.1 M cacodylate-HCl, pH 6.5, and 0.2 M NaCl | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D120A | the mutant shows 8% of wild type activity | Homo sapiens |
D120N | the mutant shows 4% of wild type activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-(2,3-dichlorophenyl)-N4-methylpyrimidine-2,4-diamine | i.e. TH287 | Homo sapiens | |
N4-cyclopropyl-6-(2,3-dichlorophenyl)pyrimidine-2,4-diamine | i.e. TH588 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
2-oxo-dATP | wild type enzyme, at pH 7.5 and 22°C | Homo sapiens | |
0.006 | - |
2-oxo-dATP | mutant enzyme D120N, at pH 7.5 and 22°C | Homo sapiens | |
0.0084 | - |
8-oxo-dGTP | wild type enzyme, at pH 7.5 and 22°C | Homo sapiens | |
0.0318 | - |
2-oxo-dATP | mutant enzyme D120A, at pH 7.5 and 22°C | Homo sapiens | |
0.0412 | - |
8-oxo-dGTP | mutant enzyme D120N, at pH 7.5 and 22°C | Homo sapiens | |
0.0444 | - |
8-oxo-dGTP | mutant enzyme D120A, at pH 7.5 and 22°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-dATP + H2O | Homo sapiens | - |
2-oxo-dAMP + diphosphate | - |
? | |
8-oxo-dATP + H2O | Homo sapiens | - |
8-oxo-dAMP + diphosphate | - |
? | |
8-oxo-dGTP + H2O | Homo sapiens | - |
8-oxo-dGMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-dATP + H2O | - |
Homo sapiens | 2-oxo-dAMP + diphosphate | - |
? | |
8-oxo-dATP + H2O | - |
Homo sapiens | 8-oxo-dAMP + diphosphate | - |
? | |
8-oxo-dGTP + H2O | - |
Homo sapiens | 8-oxo-dGMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MTH1 | - |
Homo sapiens |
NUDT1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
8-oxo-dGTP | mutant enzyme D120N, at pH 7.5 and 22°C | Homo sapiens | |
9.7 | - |
8-oxo-dGTP | mutant enzyme D120A, at pH 7.5 and 22°C | Homo sapiens | |
20.7 | - |
8-oxo-dGTP | wild type enzyme, at pH 7.5 and 22°C | Homo sapiens | |
21.1 | - |
2-oxo-dATP | wild type enzyme, at pH 7.5 and 22°C | Homo sapiens | |
21.1 | - |
2-oxo-dATP | mutant enzyme D120N, at pH 7.5 and 22°C | Homo sapiens | |
34.4 | - |
2-oxo-dATP | mutant enzyme D120A, at pH 7.5 and 22°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
8-oxo-dGTP | mutant enzyme D120N, at pH 7.5 and 22°C | Homo sapiens | |
0.2 | - |
8-oxo-dGTP | mutant enzyme D120A, at pH 7.5 and 22°C | Homo sapiens | |
1.1 | - |
2-oxo-dATP | mutant enzyme D120A, at pH 7.5 and 22°C | Homo sapiens | |
2.5 | - |
8-oxo-dGTP | wild type enzyme, at pH 7.5 and 22°C | Homo sapiens | |
3.5 | - |
2-oxo-dATP | mutant enzyme D120N, at pH 7.5 and 22°C | Homo sapiens | |
4.1 | - |
2-oxo-dATP | wild type enzyme, at pH 7.5 and 22°C | Homo sapiens |