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Literature summary for 3.6.1.55 extracted from
- Multiple enzyme activities of Escherichia coli MutT protein for sanitization of DNA and RNA precursor pools (2005), Biochemistry, 44, 6670-6674.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| MutT protein is produced as a His-tagged form in Escherichia coli M15 | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000045 | - |
8-oxo-GDP | pH 8.0, 30°C | Escherichia coli |  |
| 0.000058 | - |
8-oxo-dGDP | pH 8.0, 30°C | Escherichia coli | |
| 0.000081 | - |
8-oxo-dGTP | pH 8.0, 30°C | Escherichia coli | |
| 0.00026 | - |
8-oxo-GTP | pH 8.0, 30°C | Escherichia coli | |
| 0.17 | - |
dGDP | pH 8.0, 30°C | Escherichia coli | |
| 0.35 | - |
GDP | pH 8.0, 30°C | Escherichia coli | |
| 1 | - |
GTP | pH 8.0, 30°C | Escherichia coli | |
| 1.1 | - |
dGTP | pH 8.0, 30°C | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8-oxo-dGDP + H2O | Escherichia coli | - |
8-oxo-dGMP + phosphate | - |
? | |
| 8-oxo-dGTP + H2O | Escherichia coli | 8-oxoguanine (8-oxo-7,8-dihydroguanine) is produced in nucleic acids as well as in nucleotide pools of cells, by reactive oxygen species normally formed during cellular metabolic processes. MutT protein of Escherichia coli specifically degrades 8-oxoGua-containing deoxyribo- and ribonucleoside triphosphates to corresponding nucleoside monophosphates, thereby preventing misincorporation of 8-oxoguanine into DNA and RNA, which would cause mutation and phenotypic suppression, respectively | 8-oxo-dGMP + diphosphate | - |
? | |
| 8-oxo-GDP + H2O | Escherichia coli | - |
8-oxo-GMP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8-oxo-dGDP + H2O | - |
Escherichia coli | 8-oxo-dGMP + phosphate | - |
? | |
| 8-oxo-dGTP + H2O | 8-oxoguanine (8-oxo-7,8-dihydroguanine) is produced in nucleic acids as well as in nucleotide pools of cells, by reactive oxygen species normally formed during cellular metabolic processes. MutT protein of Escherichia coli specifically degrades 8-oxoGua-containing deoxyribo- and ribonucleoside triphosphates to corresponding nucleoside monophosphates, thereby preventing misincorporation of 8-oxoguanine into DNA and RNA, which would cause mutation and phenotypic suppression, respectively | Escherichia coli | 8-oxo-dGMP + diphosphate | - |
? | |
| 8-oxo-dGTP + H2O | the MutT protein has an ability to cleave the phosphoanhydride bond between the alpha and beta phosphate of 8-oxoguanine-containing nucleoside di- and triphosphates. 8-oxo-dGTP is hydrolysed with the highest efficiency | Escherichia coli | 8-oxo-dGMP + diphosphate | - |
? | |
| 8-oxo-GDP + H2O | - |
Escherichia coli | 8-oxo-GMP + phosphate | - |
? | |
| 8-oxo-GTP + H2O | - |
Escherichia coli | 8-oxo-GMP + diphosphate | - |
? | |
| dGDP + H2O | - |
Escherichia coli | dGMP + phosphate | - |
? | |
| dGTP + H2O | - |
Escherichia coli | dGMP + diphosphate | - |
? | |
| GDP + H2O | - |
Escherichia coli | GMP + phosphate | - |
? | |
| GTP + H2O | - |
Escherichia coli | GMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MuT | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
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