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Literature summary for 3.6.1.54 extracted from
- The UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis utilizes Mn2+ cluster for catalysis (2013), J. Biol. Chem., 288, 26987-27001.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is active without addition of detergent | Haemophilus influenzae | |
| Triton X-100 | stimulation in hydrolysis as the detergent concentration approaches the critical micelle concentration of 0.19 mM | Haemophilus influenzae |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Haemophilus influenzae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D117A | 2fold decrease in activity compared to wild-type | Haemophilus influenzae |
| D42A | 80000fold decrease in activity compared to wild-type | Haemophilus influenzae |
| D9A | 200000fold decrease in activity compared to wild-type | Haemophilus influenzae |
| H115A | 12000fold decrease in activity compared to wild-type | Haemophilus influenzae |
| H11A | 20000fold decrease in activity compared to wild-type | Haemophilus influenzae |
| H196A | 5000fold decrease in activity compared to wild-type | Haemophilus influenzae |
| R81A | 7000fold decrease in activity compared to wild-type | Haemophilus influenzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0079 | - |
UDP-2,3-diacylglucosamine | pH 8.0, 30°C | Haemophilus influenzae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 10-50fold stimulation compared to conditions without metal additive | Haemophilus influenzae | |
| Fe3+ | 10-50fold stimulation compared to conditions without metal additive | Haemophilus influenzae | |
| Mn2+ | 600fold stimulation in presence of Mn2+, optimum concentraion 2.5 mM. Presence of a di-Mn2+ cluster | Haemophilus influenzae | |
| Ni2+ | 10-50fold stimulation compared to conditions without metal additive | Haemophilus influenzae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus influenzae | P44046 | - |
- |
| Haemophilus influenzae DSM 11121 | P44046 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
pH 8.0, 30°C | Haemophilus influenzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-2,3-diacylglucosamine + H2O | - |
Haemophilus influenzae | 2,3-diacylglucosamine 1-phosphate + UMP | - |
? | |
| UDP-2,3-diacylglucosamine + H2O | - |
Haemophilus influenzae DSM 11121 | 2,3-diacylglucosamine 1-phosphate + UMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LpxH | - |
Haemophilus influenzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at. Enzyme is most active at slightly alkaline pH values and exhibits a sharp decrease in its catalytic activity at low pH | Haemophilus influenzae |
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Release 2023.1 (January 2023)
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