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Literature summary for 3.6.1.53 extracted from

  • Cabezas, A.; Ribeiro, J.; Rodrigues, J.; Lopez-Villamizar, I.; Fernandez, A.; Canales, J.; Pinto, R.; Costas, M.; Cameselle, J.
    Molecular bases of catalysis and ADP-ribose preference of human Mn2+-dependent adpribose/CDP-alcohol diphosphatase and conversion by mutagenesis to a preferential cyclic ADP-ribose phosphohydrolase (2015), PLoS ONE, 10, e0118680.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
substrate docking on a homology model of human ADPRibase-Mn suggests possible interactions of ADP-ribose with residue Cys253, within the metallo-dependent phosphatases signature with Gln27, Asn110, His111, or in unique structural regions of the ADPRibase-Mn family, residues Phe37 and Arg43 and Phe210 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C253A 10fold increase of the catalytic efficiency for cyclic ADP-ribose Homo sapiens
C253A Cys253 is hindering for cADPR phosphohydrolase activity, specific tenfold gain of efficiency with cyclic ADP-ribose Homo sapiens
F210A mutation lowers 40-70fold the catalytic efficiency for ADP-ribose, CDP-choline and 2',3'-cAMP hydrolysis, and 500fold for cyclic ADP-ribose Homo sapiens
F37A Phe37 is needed for ADP-ribose preference without catalytic effect Homo sapiens
F37A/L196A modest enhancement of the inhibitory effect over that of the F37A point mutation Homo sapiens
F37A/L196F modest enhancement of the inhibitory effect over that of the F37A point mutation Homo sapiens
F37A/L196F/C253A cyclic ADP-ribose is the best substrate, with a 8fold increase in catalytic efficiency compared to wild-type Homo sapiens
F37Y kinetic parameters similar to wild-type Homo sapiens
H111A marked decrease in efficiency with all substrates except 2',3'-cAMP Homo sapiens
H111N marked decrease in efficiency with all substrates except 2',3'-cAMP Homo sapiens
L196A modest 2-5fold decrease of catalytic efficiency with the substrates tested Homo sapiens
N110A reduction of catalytic efficiency is stronger for the hydrolysis of CDP-choline or 2',3'-cAMP than for the hydrolysis of ADP-ribose or cADPR. The decrease of kcat value is stronger for the hydrolysis of 2',3'-cAMP Homo sapiens
Q27H for ADP-ribose, modest negative effects of similar magnitude in catalysis and in substrate binding. For CDP-choline, the substitution affects only binding. For 2',3'-cAMP, the substitution affects catalysis, not binding Homo sapiens
R43A Arg43 is essential for catalysis, drastic efficiency loss of the mutant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
ADP-ribose wild-type, pH 7.5, 37°C Homo sapiens
0.077
-
ADP-ribose mutant F37Y, pH 7.5, 37°C Homo sapiens
0.078
-
ADP-ribose mutant H111N, pH 7.5, 37°C Homo sapiens
0.094
-
ADP-ribose mutant C253A, pH 7.5, 37°C Homo sapiens
0.115
-
ADP-ribose mutant R43A, pH 7.5, 37°C Homo sapiens
0.13
-
ADP-ribose mutant L196A, pH 7.5, 37°C Homo sapiens
0.14
-
ADP-ribose mutant H111A, pH 7.5, 37°C Homo sapiens
0.2
-
cyclic ADP-ribose mutant C253A, pH 7.5, 37°C Homo sapiens
0.2
-
ADP-ribose mutant Q27H, pH 7.5, 37°C Homo sapiens
0.29
-
ADP-ribose mutant N110A, pH 7.5, 37°C Homo sapiens
0.35
-
CDP-choline wild-type, pH 7.5, 37°C Homo sapiens
0.43
-
CDP-choline mutant F37Y, pH 7.5, 37°C Homo sapiens
0.46
-
cyclic ADP-ribose mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
0.47
-
CDP-choline mutant L196A, pH 7.5, 37°C Homo sapiens
0.5
-
CDP-choline mutant C253A, pH 7.5, 37°C Homo sapiens
0.78
-
cyclic ADP-ribose wild-type, pH 7.5, 37°C Homo sapiens
0.97
-
CDP-choline mutant F37A, pH 7.5, 37°C Homo sapiens
1.05
-
2',3'-cAMP mutant N110A, pH 7.5, 37°C Homo sapiens
1.15
-
ADP-ribose mutant F37A, pH 7.5, 37°C Homo sapiens
1.15
-
ADP-ribose mutant F37A/L196A, pH 7.5, 37°C Homo sapiens
1.2
-
ADP-ribose mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
1.25
-
CDP-choline mutant F37A/L196A, pH 7.5, 37°C Homo sapiens
1.25
-
2',3'-cAMP mutant H111A, pH 7.5, 37°C Homo sapiens
1.5
-
CDP-choline mutant F37A/L196F, pH 7.5, 37°C Homo sapiens
1.6
-
ADP-ribose mutant F37A/L196F, pH 7.5, 37°C Homo sapiens
1.7
-
CDP-choline mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
1.8
-
2',3'-cAMP mutant H111N, pH 7.5, 37°C Homo sapiens
2.1
-
ADP-ribose mutant F210A, pH 7.5, 37°C Homo sapiens
2.3
-
2',3'-cAMP mutant Q27H, pH 7.5, 37°C Homo sapiens
2.4
-
2',3'-cAMP wild-type, pH 7.5, 37°C Homo sapiens
2.5
-
2',3'-cAMP mutant F37Y, pH 7.5, 37°C Homo sapiens
2.6
-
2',3'-cAMP mutant C253A, pH 7.5, 37°C Homo sapiens
2.7
-
CDP-choline mutant H111N, pH 7.5, 37°C Homo sapiens
2.7
-
CDP-choline mutant Q27H, pH 7.5, 37°C Homo sapiens
3.4
-
2',3'-cAMP mutant L196A, pH 7.5, 37°C Homo sapiens
4
-
CDP-choline mutant N110A, pH 7.5, 37°C Homo sapiens
4.8
-
2',3'-cAMP mutant F37A/L196A, pH 7.5, 37°C Homo sapiens
5.1
-
2',3'-cAMP mutant F37A, pH 7.5, 37°C Homo sapiens
5.2
-
2',3'-cAMP mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
7.1
-
2',3'-cAMP mutant F37A/L196F, pH 7.5, 37°C Homo sapiens
7.5
-
CDP-choline mutant H111A, pH 7.5, 37°C Homo sapiens
7.6
-
2',3'-cAMP mutant F210A, pH 7.5, 37°C Homo sapiens
7.6
-
2',3'-cAMP mutant R43A, pH 7.5, 37°C Homo sapiens
9
-
CDP-choline mutant R43A, pH 7.5, 37°C Homo sapiens
11
-
CDP-choline mutant F210A, pH 7.5, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens W0NWJ0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cAMP + H2O
-
Homo sapiens ?
-
?
ADP-ribose + H2O
-
Homo sapiens AMP + D-ribose 5-phosphate
-
?
CDP-choline + H2O
-
Homo sapiens CMP + phosphocholine
-
?
cyclic ADP-ribose + H2O
-
Homo sapiens N1-(5-phosphoribosyl)-AMP
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
ADP-ribose mutant R43A, pH 7.5, 37°C Homo sapiens
0.025
-
CDP-choline mutant R43A, pH 7.5, 37°C Homo sapiens
0.11
-
2',3'-cAMP mutant N110A, pH 7.5, 37°C Homo sapiens
0.47
-
ADP-ribose mutant H111A, pH 7.5, 37°C Homo sapiens
0.95
-
2',3'-cAMP mutant R43A, pH 7.5, 37°C Homo sapiens
2 8 2',3'-cAMP mutant F37A/L196F, pH 7.5, 37°C Homo sapiens
2.2
-
CDP-choline mutant H111A, pH 7.5, 37°C Homo sapiens
2.3
-
ADP-ribose mutant H111N, pH 7.5, 37°C Homo sapiens
2.7
-
2',3'-cAMP mutant F210A, pH 7.5, 37°C Homo sapiens
3 6 2',3'-cAMP mutant F37A, pH 7.5, 37°C Homo sapiens
3.2
-
cyclic ADP-ribose wild-type, pH 7.5, 37°C Homo sapiens
4.3
-
ADP-ribose mutant N110A, pH 7.5, 37°C Homo sapiens
4.3
-
2',3'-cAMP mutant Q27H, pH 7.5, 37°C Homo sapiens
5.3
-
CDP-choline mutant H111N, pH 7.5, 37°C Homo sapiens
5.7
-
CDP-choline mutant N110A, pH 7.5, 37°C Homo sapiens
7
-
ADP-ribose mutant F37A/L196A, pH 7.5, 37°C Homo sapiens
7.4
-
2',3'-cAMP mutant H111A, pH 7.5, 37°C Homo sapiens
8
-
ADP-ribose mutant F37A/L196F, pH 7.5, 37°C Homo sapiens
8.9
-
cyclic ADP-ribose mutant C253A, pH 7.5, 37°C Homo sapiens
10
-
ADP-ribose mutant Q27H, pH 7.5, 37°C Homo sapiens
13
-
ADP-ribose mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
14
-
ADP-ribose mutant F37A, pH 7.5, 37°C Homo sapiens
16
-
cyclic ADP-ribose mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
16
-
ADP-ribose mutant L196A, pH 7.5, 37°C Homo sapiens
19
-
ADP-ribose mutant F210A, pH 7.5, 37°C Homo sapiens
19
-
2',3'-cAMP mutant F37A/L196A, pH 7.5, 37°C Homo sapiens
20
-
2',3'-cAMP mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
25
-
CDP-choline mutant F37A/L196A, pH 7.5, 37°C Homo sapiens
26
-
CDP-choline mutant F37A/L196F, pH 7.5, 37°C Homo sapiens
29
-
CDP-choline mutant L196A, pH 7.5, 37°C Homo sapiens
30
-
2',3'-cAMP mutant L196A, pH 7.5, 37°C Homo sapiens
33
-
CDP-choline mutant F37A, pH 7.5, 37°C Homo sapiens
34
-
2',3'-cAMP mutant H111N, pH 7.5, 37°C Homo sapiens
35
-
ADP-ribose wild-type, pH 7.5, 37°C Homo sapiens
37
-
CDP-choline mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
39
-
CDP-choline mutant Q27H, pH 7.5, 37°C Homo sapiens
50
-
CDP-choline wild-type, pH 7.5, 37°C Homo sapiens
50
-
CDP-choline mutant F210A, pH 7.5, 37°C Homo sapiens
50
-
ADP-ribose mutant F37Y, pH 7.5, 37°C Homo sapiens
60
-
2',3'-cAMP wild-type, pH 7.5, 37°C Homo sapiens
70
-
2',3'-cAMP mutant F37Y, pH 7.5, 37°C Homo sapiens
76
-
CDP-choline mutant F37Y, pH 7.5, 37°C Homo sapiens
79
-
CDP-choline mutant C253A, pH 7.5, 37°C Homo sapiens
83
-
2',3'-cAMP mutant C253A, pH 7.5, 37°C Homo sapiens
97
-
ADP-ribose mutant C253A, pH 7.5, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0001
-
cyclic ADP-ribose mutant R43A, pH 7.5, 37°C Homo sapiens
0.006
-
CDP-choline mutant R43A, pH 7.5, 37°C Homo sapiens
0.03
-
ADP-ribose mutant R43A, pH 7.5, 37°C Homo sapiens
0.07
-
cyclic ADP-ribose mutant H111A, pH 7.5, 37°C Homo sapiens
0.08
-
cyclic ADP-ribose mutant F210A, pH 7.5, 37°C Homo sapiens
0.1
-
2',3'-cAMP mutant N110A, pH 7.5, 37°C Homo sapiens
0.13
-
cyclic ADP-ribose mutant N110A, pH 7.5, 37°C Homo sapiens
0.13
-
2',3'-cAMP mutant R43A, pH 7.5, 37°C Homo sapiens
0.15
-
cyclic ADP-ribose mutant Q27H, pH 7.5, 37°C Homo sapiens
0.29
-
cyclic ADP-ribose mutant H111N, pH 7.5, 37°C Homo sapiens
0.3
-
CDP-choline mutant H111A, pH 7.5, 37°C Homo sapiens
0.4
-
2',3'-cAMP mutant F210A, pH 7.5, 37°C Homo sapiens
0.9
-
cyclic ADP-ribose mutant F37A/L196A, pH 7.5, 37°C Homo sapiens
0.9
-
cyclic ADP-ribose mutant L196A, pH 7.5, 37°C Homo sapiens
1.3
-
cyclic ADP-ribose mutant F37A, pH 7.5, 37°C Homo sapiens
1.5
-
CDP-choline mutant N110A, pH 7.5, 37°C Homo sapiens
1.6
-
cyclic ADP-ribose mutant F37A/L196F, pH 7.5, 37°C Homo sapiens
1.9
-
2',3'-cAMP mutant Q27H, pH 7.5, 37°C Homo sapiens
2
-
CDP-choline mutant H111N, pH 7.5, 37°C Homo sapiens
3
-
ADP-ribose mutant H111A, pH 7.5, 37°C Homo sapiens
3.9
-
2',3'-cAMP mutant F37A/L196F, pH 7.5, 37°C Homo sapiens
4
-
cyclic ADP-ribose wild-type, pH 7.5, 37°C Homo sapiens
4
-
CDP-choline mutant F210A, pH 7.5, 37°C Homo sapiens
4
-
2',3'-cAMP mutant F37A/L196A, pH 7.5, 37°C Homo sapiens
4
-
2',3'-cAMP mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
4.1
-
cyclic ADP-ribose mutant F37Y, pH 7.5, 37°C Homo sapiens
4.8
-
ADP-ribose mutant F37A/L196F, pH 7.5, 37°C Homo sapiens
6
-
ADP-ribose mutant F37A/L196A, pH 7.5, 37°C Homo sapiens
6
-
2',3'-cAMP mutant H111A, pH 7.5, 37°C Homo sapiens
7
-
2',3'-cAMP mutant F37A, pH 7.5, 37°C Homo sapiens
9
-
ADP-ribose mutant F210A, pH 7.5, 37°C Homo sapiens
9
-
2',3'-cAMP mutant L196A, pH 7.5, 37°C Homo sapiens
11
-
ADP-ribose mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
12
-
ADP-ribose mutant F37A, pH 7.5, 37°C Homo sapiens
14
-
CDP-choline mutant Q27H, pH 7.5, 37°C Homo sapiens
15
-
ADP-ribose mutant N110A, pH 7.5, 37°C Homo sapiens
17
-
CDP-choline mutant F37A/L196F, pH 7.5, 37°C Homo sapiens
19
-
2',3'-cAMP mutant H111N, pH 7.5, 37°C Homo sapiens
21
-
CDP-choline mutant F37A/L196A, pH 7.5, 37°C Homo sapiens
22
-
CDP-choline mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
25
-
2',3'-cAMP wild-type, pH 7.5, 37°C Homo sapiens
28
-
2',3'-cAMP mutant F37Y, pH 7.5, 37°C Homo sapiens
29
-
ADP-ribose mutant H111N, pH 7.5, 37°C Homo sapiens
32
-
2',3'-cAMP mutant C253A, pH 7.5, 37°C Homo sapiens
34
-
CDP-choline mutant F37A, pH 7.5, 37°C Homo sapiens
35.5
-
cyclic ADP-ribose mutant F37A/L196F/C253A, pH 7.5, 37°C Homo sapiens
44
-
cyclic ADP-ribose mutant C253A, pH 7.5, 37°C Homo sapiens
52
-
ADP-ribose mutant Q27H, pH 7.5, 37°C Homo sapiens
62
-
CDP-choline mutant L196A, pH 7.5, 37°C Homo sapiens
120
-
ADP-ribose mutant L196A, pH 7.5, 37°C Homo sapiens
150
-
CDP-choline wild-type, pH 7.5, 37°C Homo sapiens
160
-
CDP-choline mutant C253A, pH 7.5, 37°C Homo sapiens
180
-
CDP-choline mutant F37Y, pH 7.5, 37°C Homo sapiens
590
-
ADP-ribose wild-type, pH 7.5, 37°C Homo sapiens
650
-
ADP-ribose mutant F37Y, pH 7.5, 37°C Homo sapiens
1000
-
ADP-ribose mutant C253A, pH 7.5, 37°C Homo sapiens