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Literature summary for 3.6.1.52 extracted from

  • Steidle, E.A.; Chong, L.S.; Wu, M.; Crooke, E.; Fiedler, D.; Resnick, A.C.; Rolfes, R.J.
    A novel inositol pyrophosphate phosphatase in Saccharomyces cerevisiae Siw14 protein selectively cleaves the beta-phosphate from 5-diphosphoinositol pentakisphosphate (5PP-IP5) (2016), J. Biol. Chem., 291, 6772-6783 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3)pLysS cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C214S inactive Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 5-diphosphoinositol pentakisphosphate at pH 6.0 and 37°C Saccharomyces cerevisiae
3
-
 4-nitrophenyl phosphate at pH 6.0 and 37°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphospho-myo-inositol polyphosphate + H2O Saccharomyces cerevisiae
-
 myo-inositol polyphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4741
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-agarose resin column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Saccharomyces cerevisiae BY4741 4-nitrophenol + phosphate
-
 ?
5-diphosphoinositol pentakisphosphate + H2O
-
 Saccharomyces cerevisiae inositol hexakisphosphate + phosphate
-
 r
diphospho-myo-inositol polyphosphate + H2O
-
 Saccharomyces cerevisiae myo-inositol polyphosphate + phosphate
-
 ?
additional information no activity with inositol hexakisphosphate Saccharomyces cerevisiae ?
-
-
additional information no activity with inositol hexakisphosphate Saccharomyces cerevisiae BY4741 ?
-
-
phosphatidylinositol 3,4,5-trisphosphate + H2O poor substrate Saccharomyces cerevisiae ?
-
 ?
phosphatidylinositol 3,4-bisphosphate + H2O poor substrate Saccharomyces cerevisiae ?
-
 ?
phosphatidylinositol 3,5-bisphosphate + H2O poor substrate Saccharomyces cerevisiae ?
-
 ?
phosphatidylinositol 3-phosphate + H2O poor substrate Saccharomyces cerevisiae ?
-
 ?
phosphatidylinositol 4,5-bisphosphate + H2O poor substrate Saccharomyces cerevisiae ?
-
 ?
phosphatidylinositol 4,5-bisphosphate + H2O poor substrate Saccharomyces cerevisiae BY4741 ?
-
 ?
phosphatidylinositol 4-phosphate + H2O poor substrate Saccharomyces cerevisiae ?
-
 ?
phosphatidylinositol 4-phosphate + H2O poor substrate Saccharomyces cerevisiae BY4741 ?
-
 ?
phosphatidylinositol 5-phosphate + H2O poor substrate Saccharomyces cerevisiae ?
-
 ?
polyphosphate + H2O poor substrate Saccharomyces cerevisiae ?
-
 ?
polyphosphate + H2O poor substrate Saccharomyces cerevisiae BY4741 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
5PP-IP5 phosphatase
-
 Saccharomyces cerevisiae
diphosphoinositol phosphate phosphohydrolase
-
 Saccharomyces cerevisiae
Siw14
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00000044
-
 4-nitrophenyl phosphate at pH 6.0 and 37°C Saccharomyces cerevisiae
0.0025
-
 5-diphosphoinositol pentakisphosphate at pH 6.0 and 37°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.074
-
 5-diphosphoinositol pentakisphosphate at pH 6.0 and 37°C Saccharomyces cerevisiae
0.15
-
 4-nitrophenyl phosphate at pH 6.0 and 37°C Saccharomyces cerevisiae