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Literature summary for 3.6.1.52 extracted from

  • Trilisenko, L.V.; Andreeva, N.A.; Eldarov, M.A.; Dumina, M.V.; Kulakovskaya, T.V.
    Polyphosphates and polyphosphatase activity in the yeast Saccharomyces cerevisiae during overexpression of the DDP1 gene (2015), Biochemistry, 80, 1312-1317 .
    View publication on PubMed
Search for more literature for 3.6.1.52

Cloned(Commentary)

Cloned (Comment) Organism
gene DDP1, recombinant overexpression in Saccharomyces cerevisiae strain CRN. The initial strain CRN lacks the endopolyphosphatase PPN1, but has its own protein DDP1, which accounts for the low endopolyphosphatase activity in this strain. The recombinant DDP1 enzyme shows an endopolyphosphatase activity, the endopolyphosphatase activity of the transformant manifests itself both with long-chain polyP208 and with short-chain polyP15. Content of phosphate and polyphosphate in cells of CRN, overview Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information overexpression of yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) in Saccharomyces cerevisiae leads to significantly increased compared to the parent strain. The content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreases by 9 and 28%, respectively Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
ATP inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
diphosphate inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
additional information ADP has no effect on the endopolyphophatase activity of recombinant DDP1 Saccharomyces cerevisiae
phosphate inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
Triphosphate inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, activates Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphospho-myo-inositol polyphosphate + H2O Saccharomyces cerevisiae
-
 myo-inositol polyphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q99321
-
-
Saccharomyces cerevisiae Q99321 cf. EC 3.6.1.60 and EC 3.6.1.10
-
Saccharomyces cerevisiae ATCC 204508 Q99321 cf. EC 3.6.1.60 and EC 3.6.1.10
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Saccharomyces cerevisiae 2 phosphate
-
 ?
diphosphate + H2O
-
 Saccharomyces cerevisiae ATCC 204508 2 phosphate
-
 ?
diphospho-myo-inositol polyphosphate + H2O
-
 Saccharomyces cerevisiae myo-inositol polyphosphate + phosphate
-
 ?
additional information endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) Saccharomyces cerevisiae ?
-
-
additional information endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) Saccharomyces cerevisiae ATCC 204508 ?
-
-
polyP15 + H2O
-
 Saccharomyces cerevisiae polyP14 + phosphate
-
 ?
polyP208 + H2O
-
 Saccharomyces cerevisiae polyP207 + phosphate
-
 ?
polyphosphate15 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
polyphosphate208 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
triphosphate + H2O
-
 Saccharomyces cerevisiae diphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
DDP1
-
 Saccharomyces cerevisiae
diphosphoinositol polyphosphate phosphohydrolase
-
 Saccharomyces cerevisiae
More see also EC 3.6.1.60 and EC 3.6.1.10 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction the content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreases by 9 and 28%, respectively. The average chain length of salt-soluble and alkali-soluble fractions does not change in the overexpressing strain, and that of acid-soluble polyphosphate increases under phosphate excess. At the initial stage of polyphosphate recovery after phosphorus starvation, the chain length of the acid-soluble fraction in transformed cells is lower compared to the recipient strain. In DDP1 deletion mutant, the level of inositol pyrophosphate is twice higher, while the level of polyphosphate is reduced. The overexpression of DDP1 probably leads to a decrease in the level of diphosphoinositol pentakisphosphate and bis(diphosphoinositol) tetrakisphosphate in the cell. These compounds seem to be involved in the regulation of polyphosphate synthesis and degradation Saccharomyces cerevisiae
metabolism diphosphoinositol polyphosphate phosphohydrolase (DDP1, EC 3.6.1.52) is also a diadenosine hexaphosphate hydrolase (AMP-forming) (EC 3.6.1.60) and shows endopolyphosphatase (EC 3.6.1.10) activity. The relationship between inositol pyrophosphate and polyphosphate metabolisms seems to be complicated Saccharomyces cerevisiae
physiological function yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) having endopolyphosphatase activity on inorganic polyphosphate metabolism in Saccharomyces cerevisiae. Complex nature of DDP1 involvement in the regulation of polyphosphate content and chain length in yeasts Saccharomyces cerevisiae