Cloned (Comment) | Organism |
---|---|
gene DDP1, recombinant overexpression in Saccharomyces cerevisiae strain CRN. The initial strain CRN lacks the endopolyphosphatase PPN1, but has its own protein DDP1, which accounts for the low endopolyphosphatase activity in this strain. The recombinant DDP1 enzyme shows an endopolyphosphatase activity, the endopolyphosphatase activity of the transformant manifests itself both with long-chain polyP208 and with short-chain polyP15. Content of phosphate and polyphosphate in cells of CRN, overview | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) in Saccharomyces cerevisiae leads to significantly increased compared to the parent strain. The content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreases by 9 and 28%, respectively | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 | Saccharomyces cerevisiae | |
diphosphate | inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 | Saccharomyces cerevisiae | |
additional information | ADP has no effect on the endopolyphophatase activity of recombinant DDP1 | Saccharomyces cerevisiae | |
phosphate | inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 | Saccharomyces cerevisiae | |
Triphosphate | inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, activates | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphospho-myo-inositol polyphosphate + H2O | Saccharomyces cerevisiae | - |
myo-inositol polyphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q99321 | - |
- |
Saccharomyces cerevisiae | Q99321 | cf. EC 3.6.1.60 and EC 3.6.1.10 | - |
Saccharomyces cerevisiae ATCC 204508 | Q99321 | cf. EC 3.6.1.60 and EC 3.6.1.10 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Saccharomyces cerevisiae | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | 2 phosphate | - |
? | |
diphospho-myo-inositol polyphosphate + H2O | - |
Saccharomyces cerevisiae | myo-inositol polyphosphate + phosphate | - |
? | |
additional information | endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) | Saccharomyces cerevisiae | ? | - |
- |
|
additional information | endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) | Saccharomyces cerevisiae ATCC 204508 | ? | - |
- |
|
polyP15 + H2O | - |
Saccharomyces cerevisiae | polyP14 + phosphate | - |
? | |
polyP208 + H2O | - |
Saccharomyces cerevisiae | polyP207 + phosphate | - |
? | |
polyphosphate15 + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
polyphosphate208 + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
triphosphate + H2O | - |
Saccharomyces cerevisiae | diphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DDP1 | - |
Saccharomyces cerevisiae |
diphosphoinositol polyphosphate phosphohydrolase | - |
Saccharomyces cerevisiae |
More | see also EC 3.6.1.60 and EC 3.6.1.10 | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | the content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreases by 9 and 28%, respectively. The average chain length of salt-soluble and alkali-soluble fractions does not change in the overexpressing strain, and that of acid-soluble polyphosphate increases under phosphate excess. At the initial stage of polyphosphate recovery after phosphorus starvation, the chain length of the acid-soluble fraction in transformed cells is lower compared to the recipient strain. In DDP1 deletion mutant, the level of inositol pyrophosphate is twice higher, while the level of polyphosphate is reduced. The overexpression of DDP1 probably leads to a decrease in the level of diphosphoinositol pentakisphosphate and bis(diphosphoinositol) tetrakisphosphate in the cell. These compounds seem to be involved in the regulation of polyphosphate synthesis and degradation | Saccharomyces cerevisiae |
metabolism | diphosphoinositol polyphosphate phosphohydrolase (DDP1, EC 3.6.1.52) is also a diadenosine hexaphosphate hydrolase (AMP-forming) (EC 3.6.1.60) and shows endopolyphosphatase (EC 3.6.1.10) activity. The relationship between inositol pyrophosphate and polyphosphate metabolisms seems to be complicated | Saccharomyces cerevisiae |
physiological function | yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) having endopolyphosphatase activity on inorganic polyphosphate metabolism in Saccharomyces cerevisiae. Complex nature of DDP1 involvement in the regulation of polyphosphate content and chain length in yeasts | Saccharomyces cerevisiae |