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Literature summary for 3.6.1.52 extracted from
- Oxidation of the diphosphoinositol polyphosphate phosphohydrolase-like Nudix hydrolase Aps from Drosophila melanogaster induces thermolability--A possible regulatory switch? (2010), Int. J. Biochem. Cell Biol., 42, 1174-1181.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli M15 cells as a hexahistidine-tagged fusion protein | Drosophila melanogaster |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00007 | - |
diphosphoinositol pentakisphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster |  |
| 0.004 | - |
adenosine tetraphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 0.011 | - |
guanosine tetraphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 0.018 | - |
diadenosine 5',5'''-P1,P6-hexaphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 0.13 | - |
5-phospho-D-ribosyl 1-diphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | Aps is predominantly cytoplasmic | Drosophila melanogaster | 5737 | - |
| nucleus | - |
Drosophila melanogaster | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Co2+ supports less than 5% of the activity with Mg2+ | Drosophila melanogaster | |
| Mg2+ | Mg2+ is the most effective stimulatory cation above 10 mM | Drosophila melanogaster | |
| Mn2+ | Mn2+ is also effective, with an optimal concentration of 1 mM | Drosophila melanogaster | |
| additional information | Ca2+, Ni2+ or Zn2+ are ineffective in stimulating activity of Aps | Drosophila melanogaster |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| dithiothreitol | in the absence of dithiothreitol a 5 min pretreatment at 36°C lowers enzyme activity by 65%, with temperatures above 56°C virtually abolishing all activity, whereas in the presence of dithiothreitol a 5 min treatment at temperatures up to 76°C is without effect | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| heat lability is restored by H2O2 | Drosophila melanogaster |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Drosophila melanogaster |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adult | - |
Drosophila melanogaster | - |
| embryo | - |
Drosophila melanogaster | - |
| larva | - |
Drosophila melanogaster | - |
| pupa | - |
Drosophila melanogaster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Drosophila melanogaster | 5-phospho-alpha-D-ribose 1-phosphate + phosphate | - |
? | |
| 8-oxo-dATP + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| 8-oxo-dGTP + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| adenosine tetraphosphate + H2O | the substrate shows great hydrolysis by Aps | Drosophila melanogaster | ? | - |
? | |
| ADP-glucose + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| ADP-ribose + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| CTP + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| diadenosine 5',5'''-P1,P5-pentaphosphate + H2O | - |
Drosophila melanogaster | ? | - |
? | |
| diadenosine 5',5'''-P1,P6-hexaphosphate + H2O | the substrate shows great hydrolysis by Aps | Drosophila melanogaster | ? | - |
? | |
| diguanosine pentaphosphate + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| diphosphoinositol pentakisphosphate + H2O | - |
Drosophila melanogaster | inositol hexakisphosphate + phosphate | - |
? | |
| diphosphoinositol polyphosphate + H2O | - |
Drosophila melanogaster | inositol polyphosphate + phosphate | - |
? | |
| dTTP + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| dUTP + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| GDP-glucose + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| GDP-mannose + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| guanosine tetraphosphate + H2O | the substrate shows the greatest hydrolysis by Aps | Drosophila melanogaster | ? | - |
? | |
| additional information | no hydrolysis of diadenosine 5',5'''-P1,P3-polyphosphate, diadenosine 5',5'''-P1,P2-polyphosphate, ATP, ADP, dATP, diguanosine tetraphosphate, diguanosine triphosphate, diguanosine diphosphate, GTP, GDP, dGTP, P1-(5'-adenosyl) P5-(5'-guanosyl) polyphosphate, and P1-(5'-adenosyl) P4-(5'-guanosyl) polyphosphate | Drosophila melanogaster | ? | - |
? | |
| TDP-glucose + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| UDP-glucose + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| UDP-N-acetyl-D-galactosamine + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + H2O | low activity | Drosophila melanogaster | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aps protein | the Nudix hydrolase Aps is a product of the sole Drosophila melanogaster DIPP/Aps1/Ddp1-like gene CG6391 | Drosophila melanogaster |
| diphosphoinositol polyphosphate phosphohydrolase | - |
Drosophila melanogaster |
| DIPP | - |
Drosophila melanogaster |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | - |
at 10 mM Mg2+ | Drosophila melanogaster |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
diphosphoinositol pentakisphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 0.1 | - |
5-phospho-D-ribosyl 1-diphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 0.24 | - |
adenosine tetraphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 0.34 | - |
diadenosine 5',5'''-P1,P6-hexaphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 0.8 | - |
guanosine tetraphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
at 10 mM Mg2+ | Drosophila melanogaster |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Drosophila melanogaster | Aps expression is highest in embryos and declines throughout development | down |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
5-phospho-D-ribosyl 1-diphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 19 | - |
diadenosine 5',5'''-P1,P6-hexaphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 60 | - |
adenosine tetraphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 71 | - |
guanosine tetraphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
| 342 | - |
diphosphoinositol pentakisphosphate | in the presence of 10 mM Mg2+, at pH 9.0 and 26°C | Drosophila melanogaster | |
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