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Literature summary for 3.6.1.5 extracted from

  • Veloria, J.R.; Devkota, A.K.; Cho, E.J.; Dalby, K.N.
    Optimization of a luminescence-based high-throughput screening assay for detecting apyrase activity (2017), SLAS Discov., 22, 94-101 .
    View publication on PubMed
Search for more literature for 3.6.1.5

General Stability

General Stability Organism
0.1% DMSO and 0.01% Tween 20 have no effect on enzyme stability and activity Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
additional information development of a high-throughput screening (HTS)-compatible format for inhibitor screening, a luminescence-based detection system as cost-effective biochemical assay in microplates, overview. 0.1% DMSO and 0.01% Tween 20 have no effect on enzyme stability and activity Solanum tuberosum
orthovanadate inhibition profile at 0-10 mM Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00929
-
 ATP pH 6.0, 22°C Solanum tuberosum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, apyrase is a calcium-activated enzyme Solanum tuberosum
Mg2+ activates Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Solanum tuberosum
-
 AMP + phosphate
-
 ?
ATP + 2 H2O Solanum tuberosum overall reaction AMP + 2 phosphate
-
 ?
ATP + H2O Solanum tuberosum
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Solanum tuberosum AMP + phosphate
-
 ?
ATP + 2 H2O overall reaction Solanum tuberosum AMP + 2 phosphate
-
 ?
ATP + H2O
-
 Solanum tuberosum ADP + phosphate
-
 ?
additional information optimization of a luminescence-based high-throughput screening assay for detecting apyrase activity, overview Solanum tuberosum ?
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Solanum tuberosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
100
-
 ATP pH 6.0, 22°C Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Solanum tuberosum