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Literature summary for 3.6.1.5 extracted from

  • Ji, Y.; Adeola, O.; Strawn, T.L.; Jeong, S.S.; Chen, R.; Fay, W.P.
    Recombinant soluble apyrase APT102 inhibits thrombosis and intimal hyperplasia in vein grafts without adversely affecting hemostasis or re-endothelialization (2017), J. Thromb. Haemost., 15, 814-825 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.5

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Homo sapiens
-
 AMP + phosphate
-
 ?
ATP + 2 H2O Homo sapiens overall reaction AMP + 2 phosphate
-
 ?
ATP + H2O Homo sapiens
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Homo sapiens AMP + phosphate
-
 ?
ATP + 2 H2O overall reaction Homo sapiens AMP + 2 phosphate
-
 ?
ATP + H2O
-
 Homo sapiens ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
APT102
-
 Homo sapiens
soluble apyrase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function occlusion of vein grafts (VGs) after bypass surgery due to thrombosis and intimal hyperplasia (IH) is a major clinical problem. Apyrases are enzymes that scavenge extracellular ATP and ADP and promote adenosine formation at sites of vascular injury and hence have potential to inhibit vein graft pathology. Recombinant soluble apyrase APT102 inhibits thrombosis and intimal hyperplasia in vein grafts without adversely affecting hemostasis or re-endothelialization. Effects of recombinant soluble human apyrase, APT102, on platelets, smooth muscle cells (SMCs), and endothelial cells (ECs) in vitro and thrombosis and IH in murine VGs from C57BL/6J male mice, overview. While potently inhibiting ADP-induced platelet aggregation and VG thrombosis, APT102 does not impair surgical hemostasis. APT102 does not directly inhibit SMC or EC proliferation, but significantly attenuates the effects of ATP on SMC and EC proliferation. APT102 significantly inhibits SMC migration, but does not inhibit EC migration, which may be mediated, at least in part, by inhibition of SMC, but not EC, migration by adenosine. At 4 weeks after surgery, IH is significantly less in VGs of APT102-treated mice than in control VGs. APT102 significantly inhibit cell proliferation in VGs, but does not inhibit re-endothelialization Homo sapiens