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Literature summary for 3.6.1.5 extracted from
- First report of the characterization of a snake venom apyrase (Ruviapyrase) from Indian Russells viper (Daboia russelii) venom (2018), Int. J. Biol. Macromol., 111, 639-648 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DTT | - |
Daboia russelii |  |
| EDTA | - |
Daboia russelii | |
| iodoacetamide | - |
Daboia russelii | |
| additional information | the catalytic activity and platelet deaggregation property of Ruviapyrase is significantly inhibited by EDTA, DTT, and iodoacetamide, and neutralized by commercial monovalent and polyvalent antivenom. Poor inhibition by PMSF | Daboia russelii | |
| Zn2+ | 63% (ADPase) and 71% (ATPase) inhibition at 2 mM | Daboia russelii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00254 | - |
ATP | pH 7.4, 37°C | Daboia russelii | |
| 0.00577 | - |
ADP | pH 7.4, 37°C | Daboia russelii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Daboia russelii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Daboia russelii | |
| Mg2+ | activates, less effective than Ca2+ | Daboia russelii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83041 | - |
MALDI-TOF-mass spectrometry analysis and gel filtration | Daboia russelii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | Daboia russelii | - |
AMP + phosphate | - |
? | |
| ATP + H2O | Daboia russelii | - |
ADP + phosphate | - |
? | |
| additional information | Daboia russelii | Ruviapyrase does not show cytotoxicity against breast cancer (MCF-7) cells and haemolytic activity, it exhibits marginal anticoagulant and strong antiplatelet activity, and dose-dependently reverses the ADP-induced platelet aggregation. The catalytic activity and platelet deaggregation property of Ruviapyrase is significantly inhibited by EDTA, DTT and IAA, and neutralized by commercial monovalent and polyvalent antivenom | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daboia russelii | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme is a monomeric glycoprotein that contains 2.4% neutral sugars and 58.4% N-linked oligosaccharides and strongly binds to concanavalin A | Daboia russelii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 1.6fold (ATPase) and 2.3fold (ADPase) from venom by gel filtration and anion exchange chromatography | Daboia russelii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Daboia russelii | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| the catalytic activity (both ATPase as well as ADPase) of Ruviapyrase declines rapidly at physiological conditions (37°C, pH 7.4). It loses 80% of its enzyme activity within 2 h of incubation albeit ATPase and ADPase activities of crude Russel viper venom are found to be more stable compared to Ruviapyrase | Daboia russelii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | - |
Daboia russelii | AMP + phosphate | - |
? | |
| ATP + H2O | - |
Daboia russelii | ADP + phosphate | - |
? | |
| additional information | Ruviapyrase does not show cytotoxicity against breast cancer (MCF-7) cells and haemolytic activity, it exhibits marginal anticoagulant and strong antiplatelet activity, and dose-dependently reverses the ADP-induced platelet aggregation. The catalytic activity and platelet deaggregation property of Ruviapyrase is significantly inhibited by EDTA, DTT and IAA, and neutralized by commercial monovalent and polyvalent antivenom | Daboia russelii | ? | - |
- |
|
| additional information | Ruviapyrase hydrolysed adenosine triphosphate (ATP) to a significantly greater extent as compared to adenosine diphosphate (ADP). The enzyme is devoid of 5'-nucleotidase and phosphodiesterase activities. The specificity constant or kinetic efficiency of Ruviapyrase in hydrolysing ATP is 3.7folds higher compared to hydrolysis of ADP under identical experimental conditions | Daboia russelii | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 79400, glycosylated enzyme, SDS-PAGE, x * 33000, deglycosylated enzyme, SDS-PAGE | Daboia russelii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ruviapyrase | - |
Daboia russelii |
| snake venom apyrase | - |
Daboia russelii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Daboia russelii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 248.6 | - |
ADP | pH 7.4, 37°C | Daboia russelii | |
| 410 | - |
ATP | pH 7.4, 37°C | Daboia russelii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Daboia russelii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 43085 | - |
ADP | pH 7.4, 37°C | Daboia russelii | |
| 161417 | - |
ATP | pH 7.4, 37°C | Daboia russelii | |
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