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Literature summary for 3.6.1.5 extracted from

  • Dias, D.A.; de Barros Penteado, B.; Dos Santos, L.D.; Dos Santos, P.M.; Arruda, C.C.P.; Schetinger, M.R.C.; Leal, D.B.R.; Dos Santos Jaques, J.A.
    Characterization of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5) activity in mouse peritoneal cavity cells (2017), Cell Biochem. Funct., 35, 358-363 .
    View publication on PubMed
Search for more literature for 3.6.1.5

Inhibitors

Inhibitors Comment Organism Structure
additional information oligomycin (an inhibitor of mitochondrial ATPases), orthovanadate (an inhibitor of transport ATPases, acid phosphatases, and phosphotyrosine phosphatases), Ap5A (an inhibitor of adenylate kinase [AK]), ouabain (a classic inhibitor of Na+-ATPase and K+-ATPase), NEM (Ca2+-ATPases and Mg2+-ATPases, AK and sulfhydryl group modifier), levamisole (inhibitor of alkaline phosphatase), tetramisole (inhibitor of alkaline phosphatase), and sodium azide (inhibitor of mitochondrial ATPases) below 10 mM do not show an inhibitory effect on ATP and ADP hydrolysis by E-NTPDase Mus musculus
Sodium azide
-
 Mus musculus
Sodium fluoride
-
 Mus musculus
suramin
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
 ATP pH 8.5, 37°C Mus musculus
0.66
-
 ADP pH 8.5, 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, activates at 1.5 mM Mus musculus
Mg2+ activates at 1.5 mM Mus musculus
additional information Ca2+-ATP and Ca2+-ADP hydrolysis are superior to hydrolysis with Mg2+-ATP and Mg2+-ADP as substrates Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Mus musculus
-
 AMP + phosphate
-
 ?
ADP + H2O Mus musculus BALB/c
-
 AMP + phosphate
-
 ?
ATP + H2O Mus musculus
-
 ADP + phosphate
-
 ?
ATP + H2O Mus musculus BALB/c
-
 ADP + phosphate
-
 ?
GDP + H2O Mus musculus
-
 GMP + phosphate
-
 ?
GTP + H2O Mus musculus
-
 GDP + phosphate
-
 ?
GTP + H2O Mus musculus BALB/c
-
 GDP + phosphate
-
 ?
UDP + H2O Mus musculus
-
 UMP + phosphate
-
 ?
UTP + H2O Mus musculus
-
 UDP + phosphate
-
 ?
UTP + H2O Mus musculus BALB/c
-
 UDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus P55772
-
-
Mus musculus BALB/c P55772
-
-

Source Tissue

Source Tissue Comment Organism Textmining
B-lymphocyte
-
 Mus musculus
-
macrophage
-
 Mus musculus
-
peritoneal cavity
-
 Mus musculus
-
T-lymphocyte
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1208
-
 substrate ADP, pH 8.5, 37°C Mus musculus
0.1364
-
 substrate ATP, pH 8.5, 37°C Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Mus musculus AMP + phosphate
-
 ?
ADP + H2O
-
 Mus musculus BALB/c AMP + phosphate
-
 ?
ATP + H2O
-
 Mus musculus ADP + phosphate
-
 ?
ATP + H2O
-
 Mus musculus BALB/c ADP + phosphate
-
 ?
GDP + H2O
-
 Mus musculus GMP + phosphate
-
 ?
GTP + H2O
-
 Mus musculus GDP + phosphate
-
 ?
GTP + H2O
-
 Mus musculus BALB/c GDP + phosphate
-
 ?
additional information adenine nucleotides are the best substrates. The other nucleotides (GTP, UTP, GDP, and UDP) are also hydrolyzed when added to the reaction instead of ATP or ADP, which demonstrates a broad substrate specificity for E-NTPDase expressed on the surface of peritoneal cavity cells Mus musculus ?
-
-
additional information adenine nucleotides are the best substrates. The other nucleotides (GTP, UTP, GDP, and UDP) are also hydrolyzed when added to the reaction instead of ATP or ADP, which demonstrates a broad substrate specificity for E-NTPDase expressed on the surface of peritoneal cavity cells Mus musculus BALB/c ?
-
-
UDP + H2O
-
 Mus musculus UMP + phosphate
-
 ?
UTP + H2O
-
 Mus musculus UDP + phosphate
-
 ?
UTP + H2O
-
 Mus musculus BALB/c UDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
E-NTPDase
-
 Mus musculus
ectonucleoside triphosphate diphosphohydrolase
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Mus musculus