Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mimosa pudica |
Protein Variants | Comment | Organism |
---|---|---|
A75S | the Km value for ADP of the isoform APY2 mutant is decreased compared to the wild type enzyme, while the Km value for ATP is substantially increased. The mutant shows higher specific acitivity for ADP than ATP | Mimosa pudica |
S63A | the ratio of the velocity of ATP/ADP hydrolysis is higher for the isoform MP67 mutant (approximately 1) compared to the wild type enzyme | Mimosa pudica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mimosa pudica | G1UFQ9 | isoform MP67 | - |
Mimosa pudica | G1UFR4 | isoform APY2 | - |
Purification (Comment) | Organism |
---|---|
His-Select nickel affinity column chromatography | Mimosa pudica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | isoform MP67 demonstrates substantially higher substrate specificity for ADP than for ATP | Mimosa pudica | ? | - |
? | |
ATP + 2 H2O | isoform APY2 demonstrates slightly higher substrate specificity for ATP than for ADP | Mimosa pudica | AMP + 2 phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APY2 | isoform | Mimosa pudica |
MP67 | isoform | Mimosa pudica |
NTPDase | - |
Mimosa pudica |
nucleotide triphosphate diphosphohydrolase | - |
Mimosa pudica |