Literature summary for 3.6.1.5 extracted from

Zebisch, M.; Schaefer, P.; Lauble, P.; Straeter, N.
New crystal forms of NTPDase1 from the bacterium Legionella pneumophila (2013), Acta Crystallogr. Sect. F, 69, 257-262.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta cells	Legionella pneumophila

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using either 30% (w/v) pentaerythritol propoxylate 629 100 mM HEPES-NaOH pH 7.7, 50 mM MgCl2 or 100 mM MES-NaOH pH 5.0-6.0, 10-20% (w/v) PEG 3350 or 100 mM HEPES-NaOH pH 7.5, 1.4 M ammonium sulfate or 14% (w/v) PEG 3350, 200 mM KBr, 100 mM MES-NaOH pH 6.4 or in 100 mM Tris-HCl pH 7.3, 200 mM potassium/sodium tartrate, 18% (w/v) PEG 3350 with 10 mM sodium polytungstate	Legionella pneumophila

Crystallization (Comment)

Organism

hanging drop vapor diffusion method, using either 30% (w/v) pentaerythritol propoxylate 629 100 mM HEPES-NaOH pH 7.7, 50 mM MgCl2 or 100 mM MES-NaOH pH 5.0-6.0, 10-20% (w/v) PEG 3350 or 100 mM HEPES-NaOH pH 7.5, 1.4 M ammonium sulfate or 14% (w/v) PEG 3350, 200 mM KBr, 100 mM MES-NaOH pH 6.4 or in 100 mM Tris-HCl pH 7.3, 200 mM potassium/sodium tartrate, 18% (w/v) PEG 3350 with 10 mM sodium polytungstate

Legionella pneumophila

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GDP + H2O	Legionella pneumophila	-	GMP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Legionella pneumophila	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography, HiTrap Q column chromatography, and HiLoad S200 gel filtration	Legionella pneumophila

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP + H2O	-	Legionella pneumophila	GMP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
NTPDase1	-	Legionella pneumophila
nucleoside triphosphate diphosphohydrolase 1	-	Legionella pneumophila

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Release 2023.1 (January 2023)