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Literature summary for 3.6.1.5 extracted from

  • Okuhata, R.; Takishima, T.; Nishimura, N.; Ueda, S.; Tsuchiya, T.; Kanzawa, N.
    Purification and biochemical characterization of a novel ecto-apyrase, MP67, from Mimosa pudica (2011), Plant Physiol., 157, 464-475.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
MP67 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli mainly in inclusion bodies Mimosa pudica
MpAPY2 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli Mimosa pudica

Inhibitors

Inhibitors Comment Organism Structure
erythrosine B does not affect the ATPase activity but inhibits ADPase activity of MP67 to a minor extent Mimosa pudica
additional information recombinant MpAPY2 shows no significant effect in the presence of vanadate Mimosa pudica
Sodium fluoride inhibits the ADPase activity rather than the ATPase activity of MP67 Mimosa pudica
sodium orthovanadate has a strong inhibitory effect on ATPase activity and a weaker effect on ADPase activity of MP67 Mimosa pudica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Mimosa pudica
0.041
-
ADP pH 7.5, 25°C Mimosa pudica
0.169
-
ATP pH 7.5, 25°C Mimosa pudica

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Mimosa pudica 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
x * 52000, SDS-PAGE Mimosa pudica
67000
-
x * 67000, SDS-PAGE Mimosa pudica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Mimosa pudica
-
AMP + phosphate
-
?
ATP + 2 H2O Mimosa pudica
-
AMP + 2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mimosa pudica
-
-
-
Mimosa pudica G1UFQ9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein MP67 is glycosylated Mimosa pudica

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and concanavalin A affinity chromatography, recombinant enzyme from Escherichia coli Mimosa pudica

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Mimosa pudica
-
mesophyll
-
Mimosa pudica
-
additional information MP67 is ubiquitously distributed in various tissues, most abundantly in leaves Mimosa pudica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.8
-
substrate ATP, purified enzyme, pH 7.5, 25°C Mimosa pudica
12.8
-
substrate ATP, purified MP67, pH 7.5, 25°C Mimosa pudica
1140
-
substrate ADP, purified enzyme, pH 7.5, 25°C Mimosa pudica
1140
-
substrate ADP, purified MP67, pH 7.5, 25°C Mimosa pudica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
Mimosa pudica AMP + phosphate
-
?
ATP + 2 H2O
-
Mimosa pudica AMP + 2 phosphate
-
?
additional information the purified MP67 shows extremely high substrate specificity toward ADP in the presence of Ca2+ Mimosa pudica ?
-
?
additional information the recombinant MpAPY2 hydrolyzes ATP and ADP to the same extent Mimosa pudica ?
-
?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Mimosa pudica
? x * 67000, SDS-PAGE Mimosa pudica

Synonyms

Synonyms Comment Organism
APY2-type apyrase
-
Mimosa pudica
ecto-apyrase
-
Mimosa pudica
MP67
-
Mimosa pudica
MpAPY2
-
Mimosa pudica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mimosa pudica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mimosa pudica