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Literature summary for 3.6.1.5 extracted from

  • Hamasaki, R.; Kato, H.; Terayama, Y.; Iwata, H.; Valenzuela, J.G.
    Functional characterization of a salivary apyrase from the sand fly, Phlebotomus duboscqi, a vector of Leishmania major (2009), J. Insect Physiol., 55, 1044-1049.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.5

Application

Application Comment Organism
medicine its unique enzymatic activity makes the salivary apyrase an attractive candidate as a therapeutic agent for the treatment of thrombotic pathologies Phlebotomus duboscqi

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a thioredoxin-His-tagged fusion protein in Escherichia coli BL21 cells Phlebotomus duboscqi

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Phlebotomus duboscqi

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ strictly dependent on Ca2+ Phlebotomus duboscqi
additional information not dependent on Mg2+ Phlebotomus duboscqi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35200
-
 mature isoform Apy 2, calculated from amino acid sequence Phlebotomus duboscqi
35800
-
 mature isoform Apy 1, calculated from amino acid sequence Phlebotomus duboscqi
50000
-
 recombinant Apy 2 thioredoxin-His-tagged fusion protein, SDS-PAGE Phlebotomus duboscqi

Organism

Organism UniProt Comment Textmining
Phlebotomus duboscqi Q06K73
-
-
Phlebotomus duboscqi Q06K77
-
-

Purification (Commentary)

Purification (Comment) Organism
His SpinTrap column chromatography Phlebotomus duboscqi

Renatured (Commentary)

Renatured (Comment) Organism
using 8 M urea, 50 mM Tris-HCl (pH 8.0), 1 mM EDTA and 1 mM dithiothreitol Phlebotomus duboscqi

Source Tissue

Source Tissue Comment Organism Textmining
salivary gland
-
 Phlebotomus duboscqi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Phlebotomus duboscqi AMP + phosphate
-
 ?
ATP + 2 H2O
-
 Phlebotomus duboscqi AMP + 2 phosphate
-
 ?
additional information the enzyme does not hydrolyze AMP, GDP, CDP or UDP Phlebotomus duboscqi ?
-
 ?

Synonyms

Synonyms Comment Organism
Apy 1 isoform Phlebotomus duboscqi
Apy 2 isoform Phlebotomus duboscqi

pI Value

Organism Comment pI Value Maximum pI Value
Phlebotomus duboscqi isoform Apy 1, calculated from amino acid sequence
-
 7.4
Phlebotomus duboscqi isoform Apy 2, calculated from amino acid sequence
-
 9

General Information

General Information Comment Organism
physiological function the recombinant protein inhibits ADP- and collagen-induced platelet aggregation. Thus, this salivary protein plays an important role in the blood-feeding process in Phlebotomus duboscqi Phlebotomus duboscqi