Cloned (Comment) | Organism |
---|---|
expression of a His6-tagged Lpg1905 using plasmid pRSET:lpg1905 in Escherichia coli BL21(DE3) C41 strain | Legionella pneumophila |
Protein Variants | Comment | Organism |
---|---|---|
E159A | site-directed mutagenesis, inactive mutant | Legionella pneumophila |
additional information | following intratracheal inoculation of A/J mice, none of the Lpg1905 mutants is able to restore virulence to an lpg1905 mutant during lung infection | Legionella pneumophila |
N168A | site-directed mutagenesis, the mutant shows reduced activity due to decreased affinity for the nucleotide substrates, with a relatively increased Km 1.3fold for ATP hydrolysis and 3fold for ADP hydrolysis for the mutant enzyme, the mutant partially restores the ability of an enzyme-deficient Legionella pneumophila lpg1905 mutant strain to replicate in THP-1 macrophages | Legionella pneumophila |
Q193A | site-directed mutagenesis, inactive mutant | Legionella pneumophila |
R122A | site-directed mutagenesis, inactive mutant | Legionella pneumophila |
W384A | site-directed mutagenesis, inactive mutant | Legionella pneumophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
POM-1 | i.e. Na6[H2W12O40], a polyoxometalate | Legionella pneumophila | |
POM-6 | i.e. (NH4)18[NaSb9W21O86], a polyoxometalate | Legionella pneumophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate specificity and Michaelis-Menten kinetics, overview | Legionella pneumophila | |
0.4 | - |
ATP | pH 7.4, 37°C, recombinant enzyme | Legionella pneumophila | |
1 | - |
ADP | pH 7.4, 37°C, recombinant enzyme | Legionella pneumophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Legionella pneumophila | |
Mg2+ | activates | Legionella pneumophila | |
additional information | Lpg1905 is dependent on divalent metal cations | Legionella pneumophila | |
Zn2+ | activates | Legionella pneumophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Legionella pneumophila | Lpg1905 is essentially required for intracellular replication of Legionella pneumophila in eukaryotic cells leading to the Legionnaires disease, a severe and potentially fatal form of pneumonia | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Legionella pneumophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged Lpg1905 from Escherichia coli BL21(DE3) C41 strain by nickel affinity chromatography | Legionella pneumophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
catalytic efficiency | Legionella pneumophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + 2 H2O | - |
Legionella pneumophila | adenosine + 2 phosphate | - |
? | |
ATP + 2 H2O | slight preference for ATP as substrate | Legionella pneumophila | AMP + 2 phosphate | - |
? | |
GDP + H2O | - |
Legionella pneumophila | GMP + phosphate | - |
? | |
GTP + 2 H2O | - |
Legionella pneumophila | GMP + 2 phosphate | - |
? | |
additional information | Lpg1905 is essentially required for intracellular replication of Legionella pneumophila in eukaryotic cells leading to the Legionnaires disease, a severe and potentially fatal form of pneumonia | Legionella pneumophila | ? | - |
? | |
additional information | the enzyme shows the ability to hydrolyze nucleoside tri- and diphosphates, but has limited activity against CTP, CDP, UTP, and UDP | Legionella pneumophila | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains five apyrase conserved regions | Legionella pneumophila |
Synonyms | Comment | Organism |
---|---|---|
ecto-nucleoside triphosphate diphosphohydrolase | - |
Legionella pneumophila |
Lpg1905 | - |
Legionella pneumophila |
More | Lpg1905 is a prokaryotic member of the CD39/NTPDase1 family of enzymes | Legionella pneumophila |
NTPDase | - |
Legionella pneumophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Legionella pneumophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Legionella pneumophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | significant hydrolysis of both ATP and ADP at pH 6.5-pH 8.0, negligible hydrolysis of ATP or ADP substrates occurs at pH 9.0 or at pH 6.0 or lower | Legionella pneumophila |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
POM-6 | pH 7.4, 37°C, recombinant enzyme | Legionella pneumophila | |
0.267 | - |
POM-1 | pH 7.4, 37°C, recombinant enzyme | Legionella pneumophila |