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Literature summary for 3.6.1.5 extracted from
- Ornithodoros savignyi: Soft tick apyrase belongs to the 5'-nucleotidase family (2009), Exp. Parasitol., 122, 318-327.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis of isozyme I, phylogenetic analysis, expression of isozyme I as secreted protein in Pichia pastoris strain GS115 | Ornithodoros savignyi |
| DNA and amino acid sequence determination and analysis of isozyme II, phylogenetic analysis | Ornithodoros savignyi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
x * 67000, recombinant, secreted isozyme I, SDS-PAGE | Ornithodoros savignyi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Ornithodoros savignyi | salivary apyrases are nucleotide-metabolising enzymes that blood-feeding parasites utilise for modulation of extracellular nucleotides to prevent platelet activation and aggregation | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ornithodoros savignyi | A7LH73 | isozyme I; collected from the Upington region in the Western Cape, South Africa by sifting of sand, isozyme I | - |
| Ornithodoros savignyi | A7LH74 | isozyme II; collected from the Upington region in the Western Cape, South Africa by sifting of sand, isozyme II | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | predicted site of cleavage for the mature protein of the 50-nucleotidase isoforms, mass spectrometry | Ornithodoros savignyi |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from salivary gland by anion exchange chromatography, ultrafiltration, and cation exchange chromatography | Ornithodoros savignyi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| salivary gland | - |
Ornithodoros savignyi | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | salivary apyrases are nucleotide-metabolising enzymes that blood-feeding parasites utilise for modulation of extracellular nucleotides to prevent platelet activation and aggregation | Ornithodoros savignyi | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, recombinant, secreted isozyme I, SDS-PAGE | Ornithodoros savignyi |
| More | comparisons of predicted isozyme tertiary structure, N-terminal Edman sequence analysis and mass spectrometry, overview | Ornithodoros savignyi |
| More | comparisons of predicted isozyme tertiary structures, N-terminal Edman sequence analysis and mass spectrometry, overview | Ornithodoros savignyi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5'-nucleotidase | - |
Ornithodoros savignyi |
| ecto-5'-nucleotidase | - |
Ornithodoros savignyi |
| More | the apyrase belongs to the 5'-nucleotidase family | Ornithodoros savignyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Ornithodoros savignyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Ornithodoros savignyi |
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Release 2023.1 (January 2023)
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