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Literature summary for 3.6.1.5 extracted from

  • Kettlun, A.M.; Espinosa, V.; Garcia, L.; Valenzuela, M.A.
    Potato tuber isoapyrases: substrate specificity, affinity labeling, and proteolytic susceptibility (2005), Phytochemistry, 66, 975-982.
    View publication on PubMed
Search for more literature for 3.6.1.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.009
-
 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.012
-
 3'(2')-O-(methylanthranoyl)adenosine 5'-triphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.014
-
 3'(2')-O-(methylanthranoyl)adenosine 5'-diphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.017
-
 3'(2')-O-(methylanthranoyl)adenosine 5'-diphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.018
-
 dATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.018
-
 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.018
-
 3'(2')-O-(methylanthranoyl)adenosine 5'-triphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.019
-
 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.021
-
 thio-dATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.024
-
 1,N6-Etheno-ATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.027
-
 dTTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.028
-
 dGTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.029
-
 dCTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.031
-
 dATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.031
-
 1,N6-Etheno-ATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.032
-
 dCTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.048
-
 thio-dATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.073
-
 1,N6-etheno-ADP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.093
-
 dTTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.114
-
 1,N6-etheno-ADP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
0.133
-
 dGTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
 variety Desiree, low ATPase/ADPase ratio
-
Solanum tuberosum
-
 variety Pimpernel, high ATPase/ADPase ratio
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,N6-etheno-ADP + H2O
-
 Solanum tuberosum 1,N6-etheno-AMP + phosphate
-
 ?
1,N6-etheno-ATP + H2O
-
 Solanum tuberosum 1,N6-etheno-AMP + phosphate
-
 ?
2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate + H2O
-
 Solanum tuberosum 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-phosphate + phosphate
-
 ?
2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate + H2O
-
 Solanum tuberosum 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-phosphate + phosphate
-
 ?
3'(2')-O-(methylanthranoyl)adenosine 5'-diphosphate + H2O
-
 Solanum tuberosum 3'(2')-O-(methylanthranoyl)adenosine 5'-phosphate + phosphate
-
 ?
3'(2')-O-(methylanthranoyl)adenosine 5'-triphosphate + H2O
-
 Solanum tuberosum 3'(2')-O-(methylanthranoyl)adenosine 5'-phosphate + phosphate
-
 ?
dATP + H2O
-
 Solanum tuberosum dAMP + phosphate
-
 ?
dCTP + 2 H2O
-
 Solanum tuberosum dCMP + 2 phosphate
-
 ?
dGTP + 2 H2O
-
 Solanum tuberosum dGMP + 2 phosphate
-
 ?
dTTP + H2O
-
 Solanum tuberosum dTMP + phosphate
-
 ?
additional information introduction of large groups in the ribose does not produce steric hindrance in substrate binding, but causes a reduction in kcat-value Solanum tuberosum ?
-
 ?
thio-dATP + H2O
-
 Solanum tuberosum thio-dAMP + phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23
-
 3'(2')-O-(methylanthranoyl)adenosine 5'-diphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
70
-
 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
86
-
 1,N6-etheno-ADP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
191
-
 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
328
-
 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
455
-
 dTTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
490
-
 dCTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
573
-
 dGTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
595
-
 thio-dATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
636
-
 dATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
682
-
 1,N6-Etheno-ATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
800
-
 3'(2')-O-(methylanthranoyl)adenosine 5'-triphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
828
-
 3'(2')-O-(methylanthranoyl)adenosine 5'-diphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
965
-
 3'(2')-O-(methylanthranoyl)adenosine 5'-triphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
982
-
 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
1101
-
 thio-dATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
1384
-
 1,N6-etheno-ADP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
1642
-
 1,N6-Etheno-ATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
2174
-
 dCTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
2222
-
 dATP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
2841
-
 dTTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum
3019
-
 dGTP 30°C, pH 6.0, presence of Ca2+ Solanum tuberosum