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Literature summary for 3.6.1.5 extracted from

  • Faudry, E.; Lozzi, S.P.; Santana, J.M.; DSouza-Ault, M.; Kieffer, S.; Felix, C.R.; Ricart, C.A.; Sousa, M.V.; Vernet, T.; Teixeira, A.R.
    Triatoma infestans apyrases belong to the 5-nucleotidase family (2004), J. Biol. Chem., 279, 19607-19613.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
NaF 1 mM, inhibition Triatoma infestans
vanadate 1 mM, inhibition Triatoma infestans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ required, may be substituted by Mg2+, Mg2+ Triatoma infestans
Mg2+ required, may be substituted by Mn2+, Co2+ Triatoma infestans
Mn2+ required, may be substituted by Mg2+, Co2+ Triatoma infestans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59800
-
x * 79000, glycosylated form, SDS-PAGE, x * 59800, unglycosylated mature form, calculated Triatoma infestans
79000
-
x * 79000, glycosylated form, SDS-PAGE, x * 59800, unglycosylated mature form, calculated Triatoma infestans
88000
-
x * 88000, 82000, 79000, 68000, or 67000, SDS-PAGE Triatoma infestans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Triatoma infestans enzyme inhibits ADP-induced human platelet aggregation ?
-
?

Organism

Organism UniProt Comment Textmining
Triatoma infestans
-
five N-glycosylated isoforms of 88, 82, 79,68, and 67 kDa molecular masses
-
Triatoma infestans Q70GK8 precursor molecule, 79 kDa isoform
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylated protein Triatoma infestans
glycoprotein purification gives five N-glycosylated proteins of 88, 82, 79, 68, and 67 kDa molecular masses Triatoma infestans
proteolytic modification sequence contains a 23 amino acid signal peptide Triatoma infestans

Purification (Commentary)

Purification (Comment) Organism
salivary enzyme, purification gives five N-glycosylated proteins of 88, 82, 79, 68, and 67 kDa molecular masses Triatoma infestans

Source Tissue

Source Tissue Comment Organism Textmining
saliva
-
Triatoma infestans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1200
-
hydrolysis of ADP, 37°C, pH 8.0 Triatoma infestans
1500
-
hydrolysis of ATP, 37°C, pH 8.0 Triatoma infestans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O activity in decreasing order: ADP, IDP, CDP, GDP Triatoma infestans AMP + phosphate
-
?
ATP + 2 H2O activity in decreasing order: ATP, CTP, GTP, UTP, ITP Triatoma infestans AMP + 2 phosphate
-
?
CDP + H2O activity in decreasing order: ADP, IDP, CDP, GDP Triatoma infestans CMP + phosphate
-
?
CTP + 2 H2O activity in decreasing order: ATP, CTP, GTP, UTP, ITP Triatoma infestans CMP + 2 phosphate
-
?
GDP + H2O activity in decreasing order: ADP, IDP, CDP, GDP Triatoma infestans GMP + phosphate
-
?
GTP + 2 H2O activity in decreasing order: ATP, CTP, GTP, UTP, ITP Triatoma infestans GMP + 2 phosphate
-
?
IDP + H2O activity in decreasing order: ADP, IDP, CDP, GDP Triatoma infestans IMP + phosphate
-
?
ITP + 2 H2O activity in decreasing order: ATP, CTP, GTP, UTP, ITP Triatoma infestans IMP + 2 phosphate
-
?
additional information enzyme inhibits ADP-induced human platelet aggregation Triatoma infestans ?
-
?
additional information no substrate: nucleoside 5’-monophosphates, glycerol phosphate, glycose 6-phosphate, UDP-galactose Triatoma infestans ?
-
?
UTP + 2 H2O activity in decreasing order: ATP, CTP, GTP, UTP, ITP Triatoma infestans UMP + 2 phosphate
-
?

Subunits

Subunits Comment Organism
? x * 79000, glycosylated form, SDS-PAGE, x * 59800, unglycosylated mature form, calculated Triatoma infestans
? x * 88000, 82000, 79000, 68000, or 67000, SDS-PAGE Triatoma infestans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
both hydrolysis of ATP and ADP Triatoma infestans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
both hydrolysis of ATP and ADP Triatoma infestans