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Literature summary for 3.6.1.5 extracted from

  • Kukulski, F.; Komoszynski, M.
    Purification and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes (2003), Eur. J. Biochem., 270, 3447-3454.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-hydroxy-naphthalene-3,6-disulfonic acid
-
Sus scrofa
Ba2+
-
Sus scrofa
Cu2+
-
Sus scrofa
EDTA about 40% inhibition at 5 mM Sus scrofa
Sodium azide
-
Sus scrofa
suramin
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
ADP pH 7.6, 37°C Sus scrofa
0.097
-
ATP pH 7.6, 37°C Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Sus scrofa
Mg2+ most effective as activating cation, but not absolutely required Sus scrofa
Mn2+ activation Sus scrofa
additional information no effect: Na+ at 100 mM or K+ at 4 mM Sus scrofa
Zn2+
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
x * 72000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sus scrofa enzyme terminates P2 receptor-mediated signal transmission ?
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
neuronal cell synaptosome from brain cortex Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
pH 7.6, 37°C, presence of 1.5 mM Cu2+ Sus scrofa
4.1
-
pH 7.6, 37°C, presence of 1.5 mM Ba2+ Sus scrofa
10.8
-
pH 7.6, 37°C, presence of 5 mM EDTA Sus scrofa
19.4
-
pH 7.6, 37°C, presence of 1.5 mM Zn2+ Sus scrofa
25.6
-
pH 7.6, 37°C Sus scrofa
69.4
-
pH 7.6, 37°C, presence of 1.5 mM Ca2+ Sus scrofa
84.2
-
pH 7.6, 37°C, presence of 1.5 mM Mn2+ Sus scrofa
94.7
-
pH 7.6, 37°C, presence of 1.5 mM Mg2+ Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O 71% of activity compared to ATP Sus scrofa AMP + phosphate
-
?
ATP + 2 H2O
-
Sus scrofa AMP + 2 phosphate
-
?
CDP + H2O 42% of activity compared to ATP Sus scrofa CMP + phosphate
-
?
CTP + 2 H2O 87% of activity compared to ATP Sus scrofa CMP + 2 phosphate
-
?
GDP + H2O 61% of activity compared to ATP Sus scrofa GMP + phosphate
-
?
GTP + 2 H2O 91% of activity compared to ATP Sus scrofa GMP + 2 phosphate
-
?
ITP + 2 H2O 78% of activity compared to ATP Sus scrofa IMP + 2 phosphate
-
?
additional information enzyme terminates P2 receptor-mediated signal transmission Sus scrofa ?
-
?
TDP + H2O 44% of activity compared to ATP Sus scrofa TMP + phosphate
-
?
TTP + 2 H2O 82% of activity compared to ATP Sus scrofa TMP + 2 phosphate
-
?
UDP + H2O 56% of activity compared to ATP Sus scrofa UMP + phosphate
-
?
UTP + 2 H2O 94% of activity compared to ATP Sus scrofa UMP + 2 phosphate
-
?

Subunits

Subunits Comment Organism
? x * 72000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
860
-
ATP pH 7.6, 37°C Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.07
-
1-hydroxy-naphthalene-3,6-disulfonic acid pH 7.6, 37°C, substrate ADP Sus scrofa
0.103
-
1-hydroxy-naphthalene-3,6-disulfonic acid pH 7.6, 37°C, substrate ATP Sus scrofa
1.7
-
suramin pH 7.6, 37°C, substrate ADP Sus scrofa
1.8
-
suramin pH 7.6, 37°C, substrate ATP Sus scrofa
5
-
Sodium azide pH 7.6, 37°C, substrate ADP Sus scrofa
12
-
Sodium azide pH 7.6, 37°C, substrate ATP Sus scrofa

pI Value

Organism Comment pI Value Maximum pI Value
Sus scrofa isoelectric focusing
-
4.9